Increased urinary n-acetyl-beta-d-glucosaminidase activity in children with hydronephrosis

Skálová, Sylva; Rejtar, Pavel; Kutı́lek, Štěpán

doi:10.1590/s1677-55382007000100014

Cited by 12 publications

(9 citation statements)

References 10 publications

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“…Skalova et al [ 17 ] studied 31 children with hydronephrosis diagnosed by use of abdominal ultrasonography either prenatally or postnatally. The urinary NAG/Cr ratio was significantly higher in the patients with hydronephrosis in comparison with the reference data.…”

Section: Discussionmentioning

confidence: 99%

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Role of Urinary Levels of Endothelin-1, Monocyte Chemotactic Peptide-1, andN-Acetyl Glucosaminidase in Predicting the Severity of Obstruction in Hydronephrotic Neonates

et al. 2014

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PurposeAntenatal hydronephrosis (AH) is found in 0.5%-1% of neonates. The aim of the study was to assess the urinary concentrations of 3 biomarkers, endothelin-1 (ET-1), monocyte chemotactic peptide-1 (MCP-1), and N-acetyl-glucosaminidase (NAG) in severely hydronephrotic neonates.Materials and MethodsNeonates with a history of prenatal hydronephrosis were enrolled in the prospective study in 2 groups. Group 1 included neonates with severe forms of obstruction requiring surgical intervention and group 2 included neonates with milder forms of obstruction without any functional impairment. Fresh voided urinary levels of ET-1, MCP-1, and NAG were measured and their ratios to urinary Cr were calculated.ResultsFourty-two neonates were enrolled into the 2 groups: group 1, 24 patients (21 male, 3 female); group 2, 18 neonates (16 male, 2 female). There were no statistically significant differences between urinary ET-1, NAG, MCP-1 values, and ET-1/Cr and NAG/Cr ratios in groups 1 and 2. The urinary MCP-1/Cr ratio was significantly higher in group 1 than in group 2. For comparison of groups 1 and 2, the cut-off values were measured as 0.5709 ng/mg (sensitivity, 75%; specificity, 67%; positive predictive value [PPV], 71%; negative predictive value [NPV], 71%), 0.927 ng/mg (sensitivity, 77%; specificity, 72%; PPV, 77%; NPV, 72%), and 1.1913 IU/mg (sensitivity, 62%; specificity, 67%; PPV, 68%; NPV, 60%) for ET-1/Cr, MCP-1/Cr, and NAG/Cr ratios, respectively.ConclusionsThe urinary MCP-1/Cr ratio is significantly elevated in neonates with severe obstruction requiring surgical intervention. Based upon these results, urinary MCP-1/Cr may be useful in identification of severe obstructive hydronephrosis in neonates.

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Section: Discussionmentioning

confidence: 99%

“…NAG is a lysosomal enzyme that is abundantly present in the cells of the proximal tubule. It is considered to be a very sensitive marker of renal tubular impairment in various disease states [ 17 , 18 , 19 ]. Low NAG levels are found in normal urine as a result of the exocytosis/endocytosis transport system of the proximal tubules.…”

Section: Introductionmentioning

confidence: 99%

Role of Urinary Levels of Endothelin-1, Monocyte Chemotactic Peptide-1, andN-Acetyl Glucosaminidase in Predicting the Severity of Obstruction in Hydronephrotic Neonates

et al. 2014

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“…Skalova et al. reported significantly higher N‐acetyl‐ β ‐D‐glucosaminidase activity (NAG/Cre.) in children with hydronephrosis compared to their reference group.…”

Section: Discussionmentioning

confidence: 99%

Urine exoglycosidases are potential markers of renal tubular injury in children with ureteropelvic junction obstruction

et al. 2015

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“…The NAG has a relatively high molecular weight of approximately 130 000-140 000 Da which does not permit its filtration through the glomerular basal membrane and it is rapidly cleared from the circulation by the liver. Thus, urinary NAG originates primarily from the proximal tubule, and increased urinary excretion is a consequence of renal tubular cell breakdown, especially in acute kidney injury (Skalova et al, 2009). Now, NAG has been remarked to be one of the most sensitive biological markers of renal tubular injury.…”

Section: Discussionmentioning

confidence: 99%

The pharmacokinetics change of cefepime after Shuanghuanglian injection administration in subjects with the renal damage

Chen

Min

Zhao

et al. 2015

Drug and Chemical Toxicology

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Shuanghuanglian injection (SHLI) has been widely used for administration with cephalosporin in China for long time. The objective of this study was to evaluate the pharmacological properties and biochemical changes of cefepime combined with SHLI. The SD rats included were received either an intravenous (iv. 4 mL/kg) dose of normal saline, or intravenous (iv. 0.74, 0.37, 0.185 g/kg, respectively) doses of SHLI once daily for 7 days. After last administration, cefepime (0.41 g/kg) was intravenous injected to the animals. The serum and urine samples were acquired and stored at 4 °C. They were used for quantitative determination of urea nitrogen (BUN), creatinine (CRE), urine protein, alkaline phosphatase (ALP) and N-acetyl-B-d-glucosaminidase (NAG). At different time points, the levels of cefepime in rat plasma were estimated for pharmacokinetic measures by HPLC. Aspirin was selected as internal standard (IS). The results showed that there were positive effects by increasing the total amount of CRE, BUN, NAG and urine protein (p < 0.01 or <0.05) and decreasing the levels of ALP (especially the high dose group of SHLI with cefepime) (p < 0.01). Besides, the pharmacokinetic results indicated that cefepime was distributed as non-compartment model after intravenous administration. Compared with the corresponding values for the compounds given alone, the area under the blood drug concentration time curve (AUC0-t and AUC0-∞) was better increased in middle- and high-dose groups (pall < 0.01), the mean residence time (MRT) of cefepime was larger (pall < 0.01) and the total clearance (CL) was lower at different levels. The results mean that the duration and concentration of cefepime could be prolonged and the clearance reduced while in combination with SHLI. Furthermore, the cefepime in the three tested doses caused changes of renal tubular epithelial cells while the severity of changes mainly dependent on the specific doses. In conclusion, the results above-mentioned suggest a possible contribution of drug combination in the nephrotoxicity and biochemical alterations especially at high doses. Further, monitoring measures for the renal functions are warranted to evaluate during the combination of these two drugs.

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Increased urinary n-acetyl-beta-d-glucosaminidase activity in children with hydronephrosis

Cited by 12 publications

References 10 publications

Role of Urinary Levels of Endothelin-1, Monocyte Chemotactic Peptide-1, andN-Acetyl Glucosaminidase in Predicting the Severity of Obstruction in Hydronephrotic Neonates

Role of Urinary Levels of Endothelin-1, Monocyte Chemotactic Peptide-1, andN-Acetyl Glucosaminidase in Predicting the Severity of Obstruction in Hydronephrotic Neonates

Urine exoglycosidases are potential markers of renal tubular injury in children with ureteropelvic junction obstruction

The pharmacokinetics change of cefepime after Shuanghuanglian injection administration in subjects with the renal damage

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