Bladder drainage and glandular epithelial morphometry of the prostate in benign prostatic hyperplasia with severe symptoms

Cury, Carlos Abib; Azoubel, Reinaldo; Batigália, Fernando

doi:10.1590/s1677-55382006000200015

Cited by 11 publications

(5 citation statements)

References 20 publications

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“…For Leydig cell kariometric analysis, 50 randomly selected circular or elliptical nuclei had the larger (D) and smaller (d) diameter of the nuclei measured per rat (C‐Group, n = 6; R‐Group, n = 5, S‐Group, n = 6; R + S‐Group, n = 5). The medium diameter (M) was calculated using the formula M = (D + d)/2, while area (A) and volume (V) were obtained using the following formulas: A = π × 1/4 × M2 and V = π × 1/6 × M3, respectively (Cury, Azoubel, & Batigalia, ).…”

Section: Methodsmentioning

confidence: 99%

Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats

Silva

Borges

Rosa

et al. 2020

J of Applied Toxicology

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Many obese patients are exposed to hypolipidemic and serotonin-norepinephrine reuptake inhibitor (SNRI) drugs. Statins are one of the most marketed drugs in the world to treat dyslipidemia, while sibutramine, a SNRI drug, is prescribed in some countries to treat obesity and is detected as an additive in many adulterated weight loss supplements marketed worldwide. Previous studies reported adverse effects of isolated exposure to these drugs on male rat reproductive parameters. In the present work, we further investigated male reproductive toxicity of these drugs, administered in isolation or combination in adult rats for a longer period of treatment. Adult male rats (90 days) were treated (gavage) for 70 days with saline and dimethyl sulfoxide (control), sibutramine (10 mg/kg), rosuvastatin (5 mg/kg), or rosuvastatin combined with sibutramine. Sibutramine alone or with rosuvastatin, promoted a reduction in food intake and body weight gain, weight of the epididymis, ventral prostate and seminal vesicle; as well as decreased sperm reserves and transit time through the epididymis; androgen depletion; and increased index of cytoplasmic droplet. The rosuvastatin-treated group showed reduced frequency of ejaculation. Exposure to this drug alone or combined with sibutramine impaired epididymal morphology. Co-exposed rats had altered epididymal morphometry, and seminal vesicle and testis weights. The rats also showed decreased fertility after natural mating and a trend toward a delay in ejaculation, suggesting a small synergistic effect of these drugs.Given the greater reproductive efficiency of rodents, the results obtained in the present study raise concern regarding possible fertility impairment in men taking statins and SNRI drugs. K E Y W O R D Sfertility, male rat, reproductive toxicity, rosuvastatin, sibutramine

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Section: Methodsmentioning

confidence: 99%

Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats

Silva

Borges

Rosa

et al. 2020

J of Applied Toxicology

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“…Major ( D ) and minor ( d ) diameter of the cell nuclei were obtained using a Leica microscope DMLB (100×) coupled to a digital camera and a PC with the software Leica QWin (version 3 for Windows). Thereafter, the medium diameter ( M ) vas calculated using the formula M = (D × d)/2 and nuclear area ( A ) and volume ( V ) were obtained with the following formulas: A= π × ¼ × M 2 and V= π × ⅙ × M 3 , respectively (Cury et al, ).…”

Section: Methodsmentioning

confidence: 99%

Maternal exposure to butyl paraben impairs testicular structure and sperm quality on male rats

Guerra

Sanabria

Leite

et al. 2016

Environmental Toxicology

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Parabens are hormonally active chemicals widely used as preservatives in foods and are frequently detected in human fluids and tissues. Therefore, the objective of this study was to determine the effects of maternal butyl paraben (BP) exposure on male sexual development. Pregnant Wistar rats received corn oil (control group), or BP at doses of 10, 100, or 200 mg/kg, subcutaneously, from gestational day 12 until postnatal day 21. Our results demonstrated that developmental BP exposure significantly increased the number of adult Leydig cells and the circulating concentrations of testosterone and attenuated FSH and LH concentrations at 200 mg/kg. BP exposure adversely affected spermatogenesis kinetics at doses of 10 and 200 mg/kg and provoked a decrease in the immunostaining of EsR1 and AR at 200 mg/kg. The sperm motility was impaired at the 10 mg/kg dose, and sperm head abnormalities were increased in all BP dose groups. We suggest that BP impairs testicular structure and function in the rat, affecting sperm quality. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1273-1289, 2017.

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“…Two perpendicular diameters of each cross‐section were measured, and their measurements were determined. The height of the germinal epithelium was also determined at four equidistance from each cross‐section of the seminiferous tubules 52 …”

Section: Methodsmentioning

confidence: 99%

“…The height of the germinal epithelium was also determined at four equidistance from each crosssection of the seminiferous tubules. 52…”

Section: Leydig Cell Nuclei and Volumementioning

confidence: 99%

A mixture of glyphosate and 2,4‐D herbicides enhances the deleterious reproductive outcomes induced by Western diet in obese male mice

Valente,

de Matos Manoel,

Reis

et al. 2023

Environmental Toxicology

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The consumption of Western diet (WD) – enriched in fats and sugars – is associated with overweight, obesity and male reproductive disorders. In addition to WD intake, crops and dairy products display residues of herbicides, including glyphosate and 2,4‐D that are widely applied worldwide. The concomitant exposure to WD and herbicides – mimicking contemporary scenarios – is not fully investigated. Thus, we evaluated the effects of glyphosate and 2,4‐D, alone or in mixture, on WD‐induced alterations in the male genital system. Male C57BL6J mice were submitted to WD (chow containing 20% lard, 0.2% cholesterol, 20% sucrose, and high sugar solution with 23.1 and 18.9 g/L of D‐fructose and D‐glucose) for 6 months. Concomitantly to WD, the animals received glyphosate (0.05, 5, or 50 mg/kg/day), 2,4‐D (0.02, 2 or 20 mg/kg/day) or their mixture (0, 05 + 0.02, 5 + 2, or 50 + 20 mg/kg/day) by intragastrical administration (5×/week). Doses were based on Acceptable Daily Intake (ADIs) or No Observed Adverse Effect Level (NOAEL) values. Herbicide exposure did not alter the WD‐induced obesity, hypercholesterolemia and hyperglycemia. WD induced sperm cell abnormalities, reduced the number, volume and area of Leydig cells, enhanced the frequency of epididymal abnormalities, decreased the proliferation in both germinal and epididymal epithelia, and reduced the number of androgen receptor (AR) positive epididymal cells. Remarkably, the herbicide mixtures promoted such WD‐induced effects: increased the frequency of sperm cell and epididymal abnormalities (absence of sperm, cytoplasmic vacuoles, and clear cell hypertrophy) (5 + 2 and 50 + 20 doses); decreased Leydig cell nuclei volume and area (5 + 2 and 50 + 20 doses), reduced epididymal cell proliferation (all mixtures), and AR expression (50 + 20 dose). In addition, herbicide mixtures reduced serum testosterone levels (5 + 2 and 50 + 20 doses). Our findings indicate that the mixture of glyphosate and 2,4‐D herbicides, mimicking environmentally relevant scenarios, promotes WD‐induced changes in the male genital system.

show abstract

Bladder drainage and glandular epithelial morphometry of the prostate in benign prostatic hyperplasia with severe symptoms

Cited by 11 publications

References 20 publications

Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats

Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats

Maternal exposure to butyl paraben impairs testicular structure and sperm quality on male rats

A mixture of glyphosate and 2,4‐D herbicides enhances the deleterious reproductive outcomes induced by Western diet in obese male mice

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