Experimental model of bladder instability in rabbits

Balasteghin, Karina Tuma; Nardo, Assunta; Amaro, João Luiz; Padovani, Carlos Roberto

doi:10.1590/s1677-55382003000100013

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…Group 1 (G1, n = 15) served as clinical control with no surgical intervention. The remaining 20 animals were submitted to laparotomy, and an adjustable polyethylene bracelet was placed around the bladder neck without compression of the urethra, previously catheterized with a 10F catheter, as previously described (7). From this group, only 15 rabbits developed detrusor instability verified by cystometric studies one week after surgical intervention.…”

Section: Methodsmentioning

confidence: 99%

Structural alterations of the bladder induced by detrusor instability: experimental study in rabbits

et al. 2005

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Section: Methodsmentioning

confidence: 99%

Structural alterations of the bladder induced by detrusor instability: experimental study in rabbits

et al. 2005

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“…The rabbit bladder is a good model for investigating the physiological, histological, and biochemical properties of the functioning human bladder 23 . Similarities include similar receptor distribution in the urinary tract and comparable pharmacological responses to a wide variety of agents 24 .…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

Guelfi

Stefanetti

Zampini

et al. 2017

Sci Rep

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This study investigated the location of hyaluronic acid (HA)- and chondroitin sulphate (CS)-coated gold nanoparticles in rabbit bladder and evaluated gene expression of CD44, RHAMM and ICAM-1 receptors involved in HA and CS transport into the cell. Gold nanoparticles were synthesised by reduction of gold salts with HA or CS to form HA-AuNPs and CS-AuNPs. Bladder samples were incubated with CS-AuNPs and HA-AuNPs or without glycosaminoglycans. Transmission electron microscopy, optic microscopy and scanning electron microscopy were used to determine the location of the synthesised AuNPs. Real-time PCR was used to analyse expression of urothelial cell receptors CD44, RHAMM, ICAM-1, after ex vivo administration of CS-AuNPs and HA-AuNPs. We showed that HA-AuNPs and CS-AuNPs were located in the cytoplasm and tight junctions of urothelial umbrella cells; this appearance was absent in untreated bladders. There were no significant differences in gene expression levels for CD44, RHAMM and ICAM-1 receptors in treated versus control bladder tissues. In conclusion, we clearly showed the presence of exogenous GAGs in the bladder surface and the tight junctions between umbrella cells, which is important in the regeneration pathway of the urothelium. The GAGs-AuNPs offer a promising approach to understanding the biophysical properties and imaging of urothelial tissue.

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“…The abdominal and vesical pressures at the time the urine leakage was started around vesical catheter was confirmed. Maximal bladder capacity was considered the one that attained a filling volume enough to yield bladder contractions as previously described …”

Section: Methodsmentioning

confidence: 99%

Histopathologic and Urodynamic Effects of the Anticholinergic Drugs Oxybutynin, Tolterodine, and Trospium on the Bladder

Örgen

Deliktaş

Şahın

et al. 2015

LUTS

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Intravesical oxybutynin treatment was observed to be safe; however, a single daily dose of oxybutynin may not be sufficient to increase bladder capacity. Intravesical use of trospium and tolterodine at high doses caused epithelial destruction and diffuse inflammation in the bladder mucosa. The irritation associated with epithelial destruction and inflammation prevented an increase in bladder capacity.

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Experimental model of bladder instability in rabbits

Abstract: We observed in the group with obstruction a bladder weight 2.5 higher than normal bladders. We detected involuntary contractions in 93% of the animals in group 2, establishing this experimental model as appropriate to secondary bladder instability and partial bladder outlet obstruction.

Cited by 10 publications

References 17 publications

Structural alterations of the bladder induced by detrusor instability: experimental study in rabbits

Structural alterations of the bladder induced by detrusor instability: experimental study in rabbits

Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

Histopathologic and Urodynamic Effects of the Anticholinergic Drugs Oxybutynin, Tolterodine, and Trospium on the Bladder

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