Antioxidant action of alpha lipoic acid on the testis and epididymis of diabetic rats: morphological, sperm and immunohistochemical evaluation

Corrêa, Lanna Beatriz Neves Silva; Costa, Carlos Alberto Soares da; Ribas, José Antônio Silva; Boaventura, Gilson Teles; Chagas, Mauricio Alves

doi:10.1590/s1677-5538.ibju.2018.0774

Cited by 21 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, testis and epididymis are well known as androgen-dependent organs (Mäkelä et al, 2019). Moreover, in STZ-induced diabetic animals, studies indicated that low testosterone level is a relevant factor acting on the morphometrical alterations observed here, such as reduction in the tubular diameter, the epithelial height, and the number of Sertoli and germ cells that, in the end, influenced the diminished DSP (Kianifard et al, 2012; Korejo et al, 2016; Das et al, 2017; Corrêa et al, 2019; Souza et al, 2019). Altogether, these results confirmed the adverse effects of diabetes on the testis and epididymis of Wistar rats.…”

Section: Discussionmentioning

confidence: 83%

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

et al. 2021

View full text Add to dashboard Cite

Green tea is a popular drink used for therapeutic purposes to mitigate the consequences of diabetes. In this study, we aimed at evaluating the potential of green tea infusion to ameliorate structural and enzymatic damages caused by hyperglycemia in the testis and epididymis of Wistar rats. For that, nondiabetic and streptozotocin-induced diabetic rats (negative control and diabetes control, respectively) received 0.6 mL of water by gavage. Another set of diabetic animals received 100 mg/kg of green tea infusion diluted in 0.6 mL of water/gavage (diabetes + green tea) daily. After 42 days of treatment, the testes and epididymides were removed and processed for histopathological analysis, micromineral determination, and enzymatic assays. The results showed that treatment with green tea infusion preserved the testicular and epididymal histoarchitecture, improving the seminiferous epithelium and the sperm production previously affected by diabetes. Treatment with green tea reduced tissue damages caused by this metabolic condition. Given the severity of hyperglycemia, there was no efficacy of the green tea infusion in maintaining the testosterone levels, antioxidant enzyme activity, and microminerals content. Thus, our findings indicate a protective effect of this infusion on histological parameters, with possible use as a complementary therapy for diabetes.

show abstract

Section: Discussionmentioning

confidence: 83%

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several relevant studies demonstrate that the use of antioxidants reduced the harmful oxidative effects of chronic hyperglycemia and preserved organs and tissues [17,39]. At the end of 8 weeks, a reduction in glycemia (not statistically signifi cant) was observed in the diabetic rats supplemented with ALA compared to the non-supplemented diabetic group.…”

Section: Discussionmentioning

confidence: 97%

“…ALA is especially promising in diabetic conditions due to its antioxidant action [15]. It acts as a regulator of glucose metabolism, increasing sensitivity to peripheral insulin and aiding glycemic control [16,17]. Unlike other antioxidants such as Vitamin C and Vitamin E, ALA has an amphipathic behavior [18], making it more effi cient.…”

Section: Introductionmentioning

confidence: 99%

Reduction in Glomerular and Renal Tubular Damage in Kidneys of Diabetic Rats Supplemented with Alpha-Lipoic Acid

et al. 2022

Self Cite

View full text Add to dashboard Cite

Chronic hyperglycemia caused by diabetes results in systemic damage to the body, and the kidney ends up being the target of tissue damage. The use of antioxidants such as alpha-lipoic acid (ALA) has been shown to improve clinical and biochemical parameters in diabetic patients as they help in the prevention of oxidative stress. The present study aimed to undertake a comparative evaluation of the renal morphology of normal and diabetes-induced Wistar rats supplemented with ALA. The experiment had 4 experimental groups (n=7): a control group and a diabetic group that were fed commercial food; an alpha-lipoic group and an alpha-lipoic diabetic group that received the ALA supplement. Diabetes was induced with a single intraperitoneal injection of alloxane monohydrate administered after fasting. After 60 days of the experiment, the kidneys were excised to study the histomorphometric and stereological parameters of the glomeruli and renal tubules and the immunohistochemical parameters with a podocyte count. In the evaluation, the ALA promoted improvement in all of the relevant parameters of the glomerular areas and attenuation of tubular lesions of diabetic animals (p<0.001). Supplementation with ALA also showed beneficial effects in diabetic animals, such as reducing hyperglycemia and the damage promoted by oxidative stress alongside promoting a protective effect on the renal parenchyma. The reduction of glomerular and tubular damage delayed the advance of diabetic nephropathy and its complications, preventing renal failure in diabetic groups.

show abstract

“…The glycemic levels equal to or greater than 270 mg/ dL were satisfactory for diabetic induction (Mohasseb et al, 2011). Moreover, the serum glucose levels were quantified weekly until the end of the experiment (Corrêa et al, 2019). After 8 weeks of the establishment of diabetes, all animals (control and diabetic group) were euthanized with xylazine and ketamine.…”

Section: Methodsmentioning

confidence: 99%

Influence of Alpha Lipoic Acid Supplementation on Urinary Bladder Morphology of Diabetic Rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

Diabetes Mellitus (DM) is a condition marked by hyperglycaemia that causes systemic complications, including urinary vesicle dysfunction due to oxidative stress. Further, antioxidants, as well as alpha lipoic acid (ALA), may be a response to this pathological condition. The present study verified the action of ALA as a supplement in ration on glycemia and urinary vesicle structures of rats induced by streptozotocin. The rats were divided into 4 groups: Control (CG), Alpha Lipoic (ALAG), Diabetic control (DCG), and the Diabetic alpha lipoic (DALAG) group. For induction, the diabetic groups were initially induced with streptozotocin (dose 60 mg/kg). Subsequently, group glycemia was evaluated weekly. After 8 weeks, the rats were euthanized and the bladder was collected. The bladders were histologically processed and the slides were stained with Masson's Trichrome for the histomorphometry of epithelial height, connective and muscular tissue and coloration of PicroSirius Red for further analysis of collagen fibers of the bladder. The data of the glycemia demonstrated an inferior median in DALAG compared to DGC (p<0.01). The epithelial height and percentage of the muscle tissue were greater in DALAG compared to the DGC, but not significant. However, GDAL showed improvement in the organization of collagen fibers. In conclusion, bladder the morphology alterations caused by DM were not alleviated by the administration of ALA in 8 weeks of the experiments.

show abstract

Antioxidant action of alpha lipoic acid on the testis and epididymis of diabetic rats: morphological, sperm and immunohistochemical evaluation

Cited by 21 publications

References 24 publications

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Reduction in Glomerular and Renal Tubular Damage in Kidneys of Diabetic Rats Supplemented with Alpha-Lipoic Acid

Influence of Alpha Lipoic Acid Supplementation on Urinary Bladder Morphology of Diabetic Rats

Contact Info

Product

Resources

About