Background: Attacks of Urinary tract infections (UTIs) reached about 150 million patients every year worldwide. They are considered a major reason for morbidity in females in all age groups, older men and young boys. Objectives: To investigate the effect of Ciprofloxacin treatment during urinary tract infection (UTI) infection on the proinflammatory cytokines secretion including IL-1, IL-6, IL-10, IL-12, and TNF in the adult patients. Methodology: This prospective, observational cohort study involved 50 patients diagnosed with UTI over a period of 3 months from January to March 2019. On the day of admission (before starting ciprofloxacin treatment), all the UTI patients were submitted to an estimation of urine levels of proinflammatory cytokines including IL-1, IL-6, IL-12, IL-10, and TNF during the first 48 hours after hospital admission. Cytokine levels were estimated by enzyme-linked immunoassay (ELISA) kits (R&D