Lack of evidence of HPV etiology of prostate cancer following radical surgery and higher frequency of the Arg/Pro genotype in turkish men with prostate cancer

Abstract

Objectives The aim of this study was to assess the possible role of HPV in the development of prostate cancer (PCa) and investigate the distribution of the p53 codon 72 polymorphism in PCa in a Turkish population.Materials and methods A total of 96 tissues, which had been obtained using a radical surgery method, formalin-fixed and parafin-embedded, were used in this study. The study group consisted of 60 PCa tissues (open radical prostatectomy) and the control group contained 36 benign prostatic hyperplasia tissues (BPH) (transvesical open prostatectomy). The presence of HPV and the p53 codon 72 polymorphism was investigated in both groups using real-time PCR and pyrosequencing.Results The results of the real-time PCR showed no HPV DNA in any of the 36 BPH tissue samples. HPV-DNA was positive in only 1 of the 60 PCa samples (1.7%). The HPV type of this sample was identified as HPV-57. The distribution of the three genotypes, Arg/Arg, Arg/Pro and Pro/Pro was found to be 45.6, 45.6, and 8.8% in the PCa group and 57.1%, 34.3% and 8.6% in the control group, respectively. Compared with the control group, patients with PCa had a higher frequency of the Arg/Pro genotype and Proline allele (odds ratio (OR)=1.67, 95% confidence interval (CI)=0.68-4.09, p=0.044; OR=1.13, 95% CI=0.76-1.68, p=0.021, respectively).Conclusions The results of the study do not support the hyphothesis that prostate cancer is associated with HPV infection but indicated that Proline allele can be a risk factor in the development of PCa in the Turkish population.

Identification of correlation between human papillomavirus and prostate cancer: Bradford Hill Criteria Based Evaluation.

“…The argument that stronger associations tend to be casual is reasonable. In total, 33 case-control studies 6 were found in the literature reporting association between HPV and PC 6,8,[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][31][32][33][34][35][36][37][38][39][40][41][42][43][44] . However, only of them have reported the CI, P-values and higher HPV detection ratio in PC samples as compared to the controls except one study 6, 24, 26, 32-34, 39, 43 .…”

“…The HPV detection ratio reported in these studies was varied between 0% 7,14,19,23,29,30,44 , to 100% 8 in PC samples. In most case-control studies, the detection ratio of HPV was more significant in the PC samples than the controls 6,8,[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][31][32][33][34][35][36][37][38][39][40][41][42][43][44] . In contrast, in five studies, HPV detection ratios were greater in the controls than in the PC samples 17,25,29,31,39 .…”

“…Few of the selected studies used the PC samples only, which did not compare with normal, adjacent or benign and PC samples 7,30 . However, on the other side, few of the selected studies analyzed both normal or adjacent/benign and PC samples, and this comparison revealed higher HPV detection ratios in PC samples in studies while lower in other studies as compared to the control 6,8,[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][31][32][33][34][35][36][37][38][39][40][41][42][43][44] . However, no study has found a correlation between HPV and a certain PC subtype or histologic grade.…”

Evidence of High-Risk Human Papillomavirus in Prostate cancer: A UK based study

“…Conversely, others revealed no evident link between HR-HPV and PCa [32][33][34], hence considering HPV as an etiological factor for PCa still remains controversial. A number of studies have been conducted in 15 different countries to evaluate the presence of HR-HPV in prostate tissues.…”

Editorial Comment: Lack of Evidence of HPV Etiology of Prostate Cancer Following Radical Surgery and Higher Frequency of the Arg/Pro Genotype in Turkish Men With Prostate Cancer

“…The article’s subject is not original, the author ( 1 ) themselves recognized that there is a plenty of data about the association of HPV infection and prostate cancer risk in literature. The novelty relies in the correlation between the p53 polymorphisms and the prostate cancer prognostic factors Gleason score, pathologic stage, and ISUP grading.…”