In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy

Mittal, Amisha; Tandon, Simran; Singla, Sumit; Tandon, Chanderdeep

doi:10.1590/s1677-5538.ibju.2014.0547

Cited by 13 publications

(6 citation statements)

References 25 publications

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“…Firstly, our microscopic analysis verified that C . officinarum AE modifies COM crystal morphology: over a range of 60–120 μg dw/ml, the AE induced the formation of COM crystals characterised by a concave depression in the {100} surface and rounded interfacial angles, promoting a COM transition from hexagonal to spherical shape, in a way that recalls earlier reports with citrate and Mg 2+ [54], as well as with various other plant extracts including Terminalia arjuna and cystone [55]. Also an AE of Phyllantus niruri , an Euphorbiaceae family plant widely consumed in Brazilian folk medicine and extensively studied for its beneficial effects in the treatment of urolithiasis [56], when tested by our experimental protocol, induced similar shape modifications in CaOx monohydrate crystals (S2 Fig).…”

Section: Discussionsupporting

confidence: 77%

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In vitro effects on calcium oxalate crystallization kinetics and crystal morphology of an aqueous extract from Ceterach officinarum: Analysis of a potential antilithiatic mechanism

et al. 2019

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Ceterach officinarum Willd is a plant widespread throughout Europe and used in southern Italy as a diuretic. Beliefs in the benefits of C . officinarum aqueous extract in the treatment of calcium oxalate kidney stones are widely held. Little is known, however, about the actual mechanism of its antilithiatic action. Our results in this in vitro study corroborate C . officinarum aqueous extract as a good source of antioxidants with a high antioxidant effects. Our results also demonstrate a major impact of C . officinarum aqueous extract on in vitro induced calcium oxalate crystallization kinetics and crystal morphology, showing its critical role in kidney stone formation and/or elimination. We show that progressively increasing doses of C . officinarum aqueous extract cause a sequence of effects. A powerful inhibitory action on calcium oxalate monohydrate (COM) growth and aggregation is first observed. C . officinarum aqueous extract also appears highly effective in stimulating nucleation increasing the number and reducing the size of COM crystals, which become progressively thinner, rounded and concave in a dose-dependent manner. These shape-modified COM crystals are known to be less adherent to renal tubular cells and more easily excreted through the urinary tract preventing kidney stone formation. Further, C . officinarum aqueous extract promotes the formation of calcium oxalate dihydrate (COD) rather than the monohydrate so that, at the highest concentrations used, only COD crystals are observed, in significant greater numbers with a clear reduction in their size, in a dose-dependent manner. Furthermore, AFM analyses allowed us to reveal the presence of C . officinarum component(s) on the surfaces of COD and modified COM crystals. The crystal surface adsorbed component(s) are shown to be similarly active as the total aqueous extract, suggesting a trigger factor which may direct crystal modification towards COD forms. In urolithiasis pathogenesis COD crystals are less dangerous than the COM forms due to their lower affinity for renal tubular cells. Our results are important in understanding the mechanisms which guide the modification induced by C . officinarum on the crystallization process. Based on these data, together with no adverse toxic effect being observed on the in vitro model of human intestinal enterocytes, C . officinarum aqueous extract could represent an attractive natural therapy for the treatment of urolithiasis.

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Section: Discussionsupporting

confidence: 77%

“…These rounded COM crystals are reported to be thermodynamically less stable and exhibit decreased affinity for cell renal membranes and lower adhesion to these cells than hexagonal COM crystals [55]. These shape-modified COM crystals can be more easily excreted through the urinary tract, thus preventing kidney stone generation.…”

Section: Discussionmentioning

confidence: 99%

In vitro effects on calcium oxalate crystallization kinetics and crystal morphology of an aqueous extract from Ceterach officinarum: Analysis of a potential antilithiatic mechanism

et al. 2019

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“…It is proven that the increased sizes of the crystals on the increased concentration of the urine as a result of supersaturation get a specific crystalline structure, which gets blunted on the treatment of plant extracts indicating blunted and spherical shapes of decreased crystals sizes [28]. Such crystals would be having less affinity for adhesion to epithelial surfaces and are excreted from the urinary tract reducing kidney stone generation and aggregation [29]. The effect of crystal shape on corilagin treatment would have been the same, and thus, we observed a decrease in the crystal formation after treatment.…”

Section: Discussionmentioning

confidence: 99%

The protective role of corilagin on renal calcium oxalate crystal‐induced oxidative stress, inflammatory response, and apoptosis via PPAR‐γ and PI3K/Akt pathway in rats

Yuan

Zhang

Yin

et al. 2020

Biotechnology and Applied Biochemistry

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Kidney stones, also known as calcium oxalate (CaOx) nephrolithiasis, are often asymptomatic, leading to kidney injury and renal failure complications. Corilagin is a gallotannin found in various plants and is known to elicit various biological activities. The present study aimed to elucidate the renoprotective effect of corilagin against the rats’ renal stones deposition. The rats were induced for nephrolithiasis (CaOx deposition) using 0.75% ethylene glycol in their drinking water. Then, they were treated with corilagin at 50 and 100 mg/kg/day for 4 weeks. At the end of the experimental period, the rats were killed; blood and renal tissues were collected for various histological, biochemical, and gene expression analyses. The results demonstrated that the rats had renal calculi displaying a significant increase in serum creatinine (59.39 μmol/L) and blood urea nitrogen (19.03 mmol/L) levels compared with controls. Moreover, the malondialdehyde (13.29 nmol/mg) level was found to increase with a profound reduction in antioxidants’ activities with upregulated inflammatory cytokines. In contrast, the RT‐PCR and immunohistochemistry analysis demonstrated a substantial reduction in cell survival markers PPAR‐γ and PI3K/Akt with an apparent increase in apoptosis markers genes expressions in rats suffering from renal stones. Thus, the present study results suggest that corilagin could suppress renal CaOx crystal‐induced oxidative stress, inflammatory response, and apoptosis via PPAR‐γ and PI3K/Akt‐mediated pathway.

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“…The concentrated concoction was obtained by evaporating the solvent using a rotary evaporator. The concoction obtained was dried completely to constant weight at room temperature (Jhaet al, 2016;Mittal et al, 2015).…”

Section: Plant Materials and Preparation Of Extractsmentioning

confidence: 99%

Inhibition of calculi forming oxalate by dietary Basella rubra organs: Litholytic activity

Huy

Govindan

Sivaramakumar

et al. 2022

Braz. J. Pharm. Sci.

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The inhibition of calculi forming oxalate by dietary Basella rubra plant organs leaf and stem pod has been investigated. The weight reduction assay was studied. Also a concoction of the plant organs was tested. Leaf extract was found with considerable activity whereas the concoction seems to be not much active as the stem pod extract. Soluble oxalate of the plant organs are partially removed prior to extraction of active constituents. The active component/s seem to be a non-protein and non-tannin molecule/s that may act through inhibition of calcium accumulation there by proving the positive activity against the calculi or kidney stone. Regular consumption of leaf and stem pod extracts of our plant would be helpful in calculi prophylaxis.

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In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy

Cited by 13 publications

References 25 publications

In vitro effects on calcium oxalate crystallization kinetics and crystal morphology of an aqueous extract from Ceterach officinarum: Analysis of a potential antilithiatic mechanism

In vitro effects on calcium oxalate crystallization kinetics and crystal morphology of an aqueous extract from Ceterach officinarum: Analysis of a potential antilithiatic mechanism

The protective role of corilagin on renal calcium oxalate crystal‐induced oxidative stress, inflammatory response, and apoptosis via PPAR‐γ and PI3K/Akt pathway in rats

Inhibition of calculi forming oxalate by dietary Basella rubra organs: Litholytic activity

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