Short hairpin RNA targeting insulin-like growth factor binding protein-3 restores the bioavailability of insulin-like growth factor-1 in diabetic rats

Abstract

Purpose To investigate whether intracavernosal injection of short hairpin RNA for IGFBP-3 could improve erectile function in streptozotocin-induced diabetic rats.Materials and methods After 12 weeks of IGFBP-3 short hairpin RNA injection treatment, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 and IGF-1 at mRNA and protein levels were detected by quantitative real-time PCR analysis and Western blot, respectively. The concentration of cavernous cyclic guanosine monophosphate was detected by enzyme-linked immunosorbent assay.Results At 12 weeks after intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic group (P<0.05). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. At the same time, cavernous IGF-1 expression was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01). Cavernous cyclic guanosine monophosphate concentration was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01).Conclusions Gene transfer of IGFBP-3 shRNA could improve erectile function via the restoration of cavernous IGF-1 bioavailability and an increase of cavernous cGMP concentration in the pathogenesis of erectile dysfunction in streptozotocin-induced diabetic rats.

A V2O5/Conductive‐Polymer Core/Shell Nanobelt Array on Three‐Dimensional Graphite Foam: A High‐Rate, Ultrastable, and Freestanding Cathode for Lithium‐Ion Batteries

“…So far, numerous V 2 O 5 nanostructures have been prepared and decent electrochemical performance has been demonstrated in these systems (see a complete list in Table S3 in the Supporting Information). V 2 O 5 ‐based composite materials with nanocarbon have also been reported, for example, V 2 O 5 /carbon (porous carbon, carbon nanotubes, and reduced graphene oxide, rGO) and V 2 O 5 /conducting polymer . However, in all this work, the V 2 O 5 is in powder form and thus needs to be mixed with additives (e.g., carbon black) and polymer binders, and finally compressed onto a current collector (Al foil).…”

Mesenchymal Stem Cells Modified With Short Hairpin RNA (shRNA) Constructs Targeting Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Improve Erectile Dysfunction in old Rats

“…Zhou. et al [18][19][20] recently observed that plasmid-based shRNA targeting IGFBP-3 improve diabetic related ED in the rat by effecting the testosterone concentrations and rehabilitating the dyslipidaemia.…”

Mesenchymal Stem Cells Modified with Short Hairpin RNA (shRNA) Constructs Targeting Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Improve Erectile Dysfunction in Old Rats

“…Zhou. et al[18][19][20]recently observed that plasmid-based shRNA targeting IGFBP-3 improve diabetic related ED in the rat by effecting the testosterone concentrations and rehabilitating the dyslipidaemia.Transplantation of mesenchymal stem cells (MSCs) to treat ED is a promising new therapeutic approach. Depending on the different stimuli or signals, MSCs can differentiation into various specialized cell types, such as smooth muscles, which is benefit for correcting ED[21].…”