Expression profile of microrna-145 in urothelial bladder cancer

Abstract

Purpose: Bladder cancer (BC) is the second most common malignancy of the urinary tract, with high mortality. The knowledge of the molecular pathways associated with BC carcinogenesis is crucial to identify new diagnostic and prognostic biomarkers. MicroRNAs (miRNAs) are short non-coding RNA molecules that play important roles in the regulation of gene expression by acting directly on mRNAs. miR-145 has been considered as a tumor suppressor, which targets the c-MYC, MUC-1 and FSCN1 genes. Our aim was to evaluate the expression profile of miR-145 in low-grade non-invasive and high-grade invasive bladder urothelial carcinomas. Materials and Methods:We studied 30 specimens of low-grade, non-invasive pTa and 30 of pT2/pT3 high-grade invasive UC obtained by transurethral resection or radical cystectomy, followed over a mean time of 16.1 months. Normal controls were represented by five samples of normal bladder biopsy from patients who underwent retropubic prostatectomy to treat BPH. miRNA extraction and cDNA generation were performed using commercial kits. Analysis was performed by qRT-PCR, and miR-145 expression was calculated using the 2-ΔΔct method; we used RNU-43 and RNU-48 as endogenous controls. Results: miR-145 was under-expressed in 73.3% and 86.7% of pTa and pT2/pT3, respectively, with expression means of 1.61 for the former and 0.66 for the last. There were no significant differences in miR-145 expression and histological grade, tumor stage, angiolymphatic neoplastic invasion and tumor recurrence. Conclusion: miR-145 is under-expressed in low-grade, non-invasive and high-grade invasive urothelial bladder carcinoma and may play an important role in the carcinogenesis pathway, being an interesting candidate diagnostic marker.

Quantifying the Landscape for Development and Cancer from a Core Cancer Stem Cell Circuit

“…We assume that high P53/miR-200/miR-145 expression represents normal cell states, whereas low P53/miR-200/miR-145 expression represents cancer-related cell states. Some experimental work showed that miR-200 and miR-145 are effective marker genes for cancer phenotypes (36,37). In the same way, we treat ZEB and OCT4 as the markers for stemness, which means high ZEB/OCT4 expression represents stem cell related state and low ZEB/OCT4 expression represents non-stem-related state.…”

microRNA-99a inhibiting cell proliferation, migration and invasion by targeting fibroblast growth factor receptor 3 in bladder cancer

“…Currently, the main therapeutic method for bladder cancer without metastasis is surgery followed by postoperative intravesical instillation. However, >30% of patients either fail to respond to treatment or suffer recurrent disease within five years, and 50% of patients die from metastatic disease (4). Thus, novel treatments are required to improve the prognosis for patients with bladder cancer.…”

miR‐145 is a potential biomarker for predicting clinical outcome in glioblastomas

“…Studies have shown that miR-145 functions in tumor inhibition and participates in the occurrence and development of tumors; furthermore, it was found to be downregulated in tumor tissues of various cavity viscera, such as colon cancer, bladder cancer, gastric cancer, and cervical cancer, and it is also downregulated in solid tumors, such as prostate cancer, lung cancer, liver cancer, and ovarian cancer. [16][17][18][19][20] In the present study, we investigated the miR-145 expression in 86 samples of human glioblastomas and in 36 samples of normal brain tissues isolated from patients with sTBI. We found that miR-145 expression was significantly decreased in the glioblastoma tissue samples compared with the normal brain tissue samples, which is consistent with previous reports.…”