Establishment of sensitive and resistant variety of tomato on the basis of photosynthesis and antioxidative enzymes in the presence of cobalt applied as shotgun approach

Abstract

cobalt (co) affords both beneficial as well as toxic effects to plants. the present study was performed with an aim to find out the varietal differences among five tomato cultivars against the co induced changes in growth, photosynthesis, nitrate reductase (e.c.1.6.6.1), carbonic anhydrase (e.c.4.2.1.1), antioxidative enzymes i.e. peroxidase (e.c.1.11.1.7), catalase (e.c.1.11.1.6), superoxide dismutase (e.c.1.15.1.1) and that of proline content. seeds of tomato (varieties, K-25, Nts-9, NBr-Uday, sarvodya, and Malti) were soaked in 0, 100, 200 or 300 μM cocl 2 for 0, 4, 8, 12 h (shotgun approach) and sampled at 30 days after sowing. all the varieties showed significantly different response to different treatment combinations. Despite substantial varietal difference, increased co concentration caused concomitant decrease in growth, photosynthesis and the activity of nitrate reductase and carbonic anhydrase. however, the activity of antioxidant enzymes and that of proline content increased with the increased concentration of co as well as duration of soaking in all the varieties. Out of the varieties, K-25 possessed maximum antioxidative enzyme and proline content that represent its most resistant nature against the toxic effect of co. the order of susceptibility/ sensitivity was K-25 > Nts-9 > NBr-Uday > sarvodya > Malti.

Effect of cobalt chloride on soybean seedlings subjected to cadmium stress

“…There are reports stating that both applied metals, Cd and Co, cause inhibition of nitrate reductase activity after long treatment times [17,[39][40][41]. Interestingly, the present study shows that short-term cadmium stress causes induction of NR gene, which is strongly augmented by Co (Fig.…”

“…Results which showed no statistically significant differences are marked with the same letter (a or b). to DNA damage as well as decrease in RNA levels [16][17][18][19][20].…”

“…However, although relatively low concentration of Co might be beneficial for plants, higher concentrations exhibit toxic effect [15,16]. This metal has been shown to inhibit plants growth, cause oxidative stress, DNA damage and disturbances in photosynthesis [16][17][18][19][20]. Excess of Co also leads to alterations in germination, sex ratio, photoperiodism and uptake of other elements [16].…”

Role of Ethylene and Its Cross Talk with Other Signaling Molecules in Plant Responses to Heavy Metal Stress

“…However, in soybean (Glycine max) seedlings, coapplication of Co and Cd negatively affected cell viability as well as the expression of Cd-induced genes encoding MAPK KINASE2, DNA BINDING WITH ONE FINGER1 (DOF1), and BASIC LEUCINE ZIPPER2 (bZIP2) transcription factors, suggesting that Co increased Cd toxicity to soybean plants and that this happened independently from ethylene action (Chmielowska-Bąk et al, 2014). Moreover, excessive Co also increased oxidative stress and photosynthesis inhibition as well as caused alterations in germination, sex ratio, photoperiodism, and uptake of other elements (Yıldız et al, 2009;Hasan et al, 2011). Therefore, the use of Co as an ethylene biosynthesis inhibitor in research should be interpreted with caution.…”

Вплив рийної функції Mammalia на активність аланінамінотрансферази в листках Glechoma hederacea в умовах кадмієвого забруднення

“…al., 2013) та флуоресценція як індикатор сольового стресу (Bacarin, et. al., 2011); з'ясовані механізми формування чутливості та протидії Со як стресу у різних сортів томату на базі фотосинтетичних та антиоксидантних ензимів (Hasan et al, 2011). Подібні дослідження торкалися також об'єктів тваринного походження: було досліджено пуголовки Gobiocypris rarus як потенційні біомаркери за умов дії Hg, що спричинив оксидативну генну експресію ; представники устриць Crassostrea corteziensis та Crassostrea palmula за присутності Сd, Сu, Hg, Pb та Zn (Páez-Osuna, 2015).…”