Epibrassinolide regulated synthesis of polyamines and auxins in Raphanus sativus L. seedlings under Cu metal stress

Pal, Sikander; Bhardwaj, Renu; Gupta, B. K.; Dutt, Prabhu; Kanwar, Mukesh Kumar; Arora, Priya

doi:10.1590/s1677-04202009000100004

Cited by 11 publications

(3 citation statements)

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“…Enhanced activities of these enzymes in turn suggests active involvement of SOD in the removal of superoxide (O 2 Á) radicals and H 2 O 2 by GPOX, CAT, and GR, respectively (Andre et al 2010). Our findings are supported by the observations of Choudhary et al (2009) that EBL application could significantly alleviate Cu stress in radish seedlings by improving the activities of antioxidant enzymes (CAT, SOD and GPOX). Similarly, application of putrescine had also been shown to ameliorate NaCl stress in chickpea plants through elevating the activities of CAT, GPOX, GR and SOD (Sheokand et al 2009).…”

Section: Discussionsupporting

confidence: 82%

Enhancing effects of 24-epibrassinolide and Putrescine on the antioxidant capacity and free radical scavenging activity of Raphanus sativus seedlings under Cu ion stress

et al. 2010

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The present investigation recorded significant restoration of seedling growth (root length, shoot length and fresh weight) upon application of 24-epibrassinolide (EBL) and putrescine (Put) to 7-day-old seedlings of Raphanus sativus L. cv. Pusa chetki grown under copper (Cu) ion stress. EBL and Put with/or without Cu ion treated seedlings showed increased titers of ascorbic acid, total phenols and proline when compared with Cu-stressed seedlings. Differential responses in the activities of guaiacol peroxidase (GPOX) and catalase (CAT) were noted for EBL and Put alone or with/or without Cu ion treatment. Decreased activities of glutathione reductase (GR) and superoxide dismutase (SOD) noted for EBL and Put alone were observed to enhance significantly when applied in combination with Cu ion solution. A remarkable decrease in malondialdehyde contents was observed in seedlings treated with EBL and Put alone and with/or without Cu ion stress. Enhanced free radical scavenging activities were also recorded for seedlings given EBL and Put alone or in combination over Cu ion stressed seedlings. Maximum DPPH activity was observed in seedlings treated with Put and EBL 10 -9 M ? Put. Significant enhancements in deoxyribose and reducing power activities were too recorded for Put, EBL and Put ? 10 -9 M EBL treatments. Improved seedling growth, antioxidant levels (ascorbic acid, total phenols and proline) and enzymic (GPOX, CAT, SOD and GR) activities and free radical scavenging capacities along with reduced membrane damage in seedlings given EBL and Put with/or without Cu stress suggests significant and positive interactions of EBL and Put in alleviating the Cu ion induced oxidative damage in radish seedlings.

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Section: Discussionsupporting

confidence: 82%

Enhancing effects of 24-epibrassinolide and Putrescine on the antioxidant capacity and free radical scavenging activity of Raphanus sativus seedlings under Cu ion stress

et al. 2010

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“…Choudhary et al . ( 2009 ) showed that coapplication of 24-epiBL and Cu to Raphanus sativus seedlings resulted in the reduction of putrescine and spermidine contents. However, Cu treatment increased levels of polyamines when compared to control seedlings.…”

Section: Discussionmentioning

confidence: 99%

Suppression of Chlorella vulgaris Growth by Cadmium, Lead, and Copper Stress and Its Restoration by Endogenous Brassinolide

Bajguz

2010

Arch Environ Contam Toxicol

155

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Brassinosteroids play a significant role in the amelioration of various abiotic and biotic stresses. In order to elaborate their roles in plants subjected to heavy metals stress, Chlorella vulgaris cultures treated with 10−8 M brassinolide (BL) were exposed to 10−6–10−4 M heavy metals (cadmium, lead and copper) application. Under heavy metals stress, the growth and chemical composition (chlorophyll, monosaccharides, and protein content) have been decreased during the first 48 h of cultivation. The inhibitory effect of heavy metals on C. vulgaris cultures was arranged in the following order: copper > lead > cadmium. C. vulgaris cultures treated with BL in the absence or presence of heavy metals showed no differences in the endogenous level of BL. On the other hand, treatment with heavy metals results in BL level very similar to that of control cell cultures. These results suggest that the activation of brassinosteroids biosynthesis, via an increase of endogenous BL, is not essential for the growth and development of C. vulgaris cells in response to heavy metals stress. Simultaneously, BL enhanced the content of indole-3-acetic acid, zeatin, and abscisic acid in cultures treated with heavy metals. Levels per cell of chlorophylls, protein, and monosaccharides are all increased by BL treatment when compared to nontreated control cells. Application of BL to C. vulgaris cultures reduced the accumulation of heavy metals stress on growth, prevented chlorophyll, monosaccharides, and protein loss, and increased phytochelatins content. The arrested growth of C. vulgaris cells treated with heavy metals was restored by the coapplication of BL. It suggested that BL overcame the inhibitory effect of heavy metals. From these results, it can be concluded that BL plays the positive role in the alleviation of heavy metals stress.

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“…The deleterious effects of Cu are thought to be diminished by the potential of PAs to modulate the AOX system (Paul et al, 2018). Choudhary et al (2009) have shown that Cu toxicity could be ameliorated with the exogenous application of 24‐epibrassinolide, partially because of its interaction with the biosynthetic pathways of PAs, IAA and ABA. In line with this, the pre‐treatment of wheat plants with 1 mM Spd helped to revert Cu toxicity, by lowering Cu accumulation and oxidative damage and enhancing the AOX efficiency (Agami, 2016).…”

Section: Case Studies On the Contribution Of Pas In Plants Under Hm‐smentioning

confidence: 99%

Polyamines as key regulatory players in plants under metal stress—A way for an enhanced tolerance

Spormann

Soares

Teixeira

et al. 2021

Annals of Applied Biology

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Polyamine (PA) metabolism and functions have started to raise attention from plant scientists in the last years. PAs have been investigated for their involvement in plant cell signalling and protection, proving that alterations in their endogenous levels can affect plant growth, development and survival. The recognised roles of PAs in metal‐stressed plants are presented and discussed on a “case‐study” basis, for each metal. Bearing in mind that the contamination of soils by heavy metals (HMs) is a growing problem worldwide, it is important to find efficient mechanisms through which agricultural productivity and food quality are safeguarded in a scenario of increased pollution. Making cultivars more tolerant to HM‐stress, capable of detoxifying or accumulating them safely, is a goal that will most certainly benefit from researching the functions and applicability of PAs. To date, plant PAs have been recognised for their roles as membrane‐, protein‐ and nucleic acid‐stabilisers, as protectors of cellular integrity and photosynthetic machinery, as direct and indirect signalling agents, and as emerging members of the non‐enzymatic antioxidant system. Moreover, PAs are not only important in normal plant developmental processes but have also been suggested to induce stress priming, to act as epigenetic regulators of gene expression and to enhance the detoxification and vacuolar compartmentalization of HMs. Although the stress‐ameliorating effects of PAs have been widely studied for several abiotic stresses, not much is known regarding their effects on metal induced‐stress, except for Cd. This review summarises the available work on the effects of PAs in plants exposed to Cu, Cd, Fe, Mn, Cr, Ni, Hg, Al, Pb and Zn.

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Epibrassinolide regulated synthesis of polyamines and auxins in Raphanus sativus L. seedlings under Cu metal stress

Cited by 11 publications

References 50 publications

Enhancing effects of 24-epibrassinolide and Putrescine on the antioxidant capacity and free radical scavenging activity of Raphanus sativus seedlings under Cu ion stress

Enhancing effects of 24-epibrassinolide and Putrescine on the antioxidant capacity and free radical scavenging activity of Raphanus sativus seedlings under Cu ion stress

Suppression of Chlorella vulgaris Growth by Cadmium, Lead, and Copper Stress and Its Restoration by Endogenous Brassinolide

Polyamines as key regulatory players in plants under metal stress—A way for an enhanced tolerance

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