Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7 and F8 generations

Abstract

This work aimed to identify the quantitative trait loci (QTL) associated with photosynthesis and growth and productivity traits of soybean and to study possible associations between these traits by the analysis of coincidence of QTL in linkage groups (LGs). Thus, populations of recombinant inbred lines (RILs) of the F7 and F8 generations derived from the cross between the varieties BARC-8 and Garimpo were used. The traits evaluated were net assimilation rate of CO2 under saturating light (Asat), potential photosynthesis rate (Pmax), leaf area (A), specific leaf area (SLA), specific leaf nitrogen (N); root (W R), nodule (W N), stem (W ST), leaf (W L), pod (W P) and plant dry mass (W T); nodule (nN), seed (n s), and pod number (nP); seed fresh mass per plant (W S), one-hundred seed fresh mass (W HS) and seed protein percentage (P%). It was possible to identify the following QTL associated with the following soybean traits: SLA, Asat, N, W R, W ST, W L, W T, W P, W HS, n s and nP, indicating that the RIL population has a great potential for mapping loci associated with quantitative traits of the soybean crop. The correlations between the soybean traits were partially confirmed by coincidence of QTL.

QTL mapping for yield components and agronomic traits in a Brazilian soybean population

“…Broad-sense heritability values between traits ranged from 44.92 to 86.59%, including medium and high values. Estimates close to those found for the same yield traits are reported in other studies on soybeans (Pooprompan et al 2006, Vieira et al 2006, Kim et al 2012). Higher heritability coefficient was observed for the traits NDM, NNM and W100S, indicating that the phenotypic selection efficiency can be high, since the heritability expresses the proportion of total variance, which is attributed to the genetic variation (Falconer and Mackay 1996).…”

Mapping quantitative trait loci associated with chlorophyll a fluorescence parameters in soybean (Glycine max (L.) Merr.)

“…Using a RIL population derived from soybean varieties BARC-8 and Garimpo, Vieira et al (2006) detected two QTLs for net assimilation rate of CO 2 . These QTLs were located in intervals satt241-satt345 on LG O and satt215-satt183 on LG J, respectively.…”

“…Many studies have investigated the relationships between physiological traits through QTL mapping (Lebreton et al 1995;Quarrie et al 1997;Thumma et al 2001). QTLs for photosynthetic rate (P N ) have been identified in many crops, including sunflower (Herve et al 2001), soybean (Vieira et al 2006), and rice (Teng et al 2004). P N is a final in vivo photosynthetic activity that depends on many traits (parameters).…”

Location and transmission of QTL for multiple traits in the pedigree of soybean cultivars

“…A number of studies on mapping quantitative trait loci (QTL) have published for soybean in the experimental crosses (e.g., Mansur et al 1993;Lee et al 1996a, b, c;Concibido et al 2004;Vieira et al 2006;Guzman et al 2007), but no report in the pedigree has been found. Grupe et al (2001) propose in silico QTL-mapping method for analyzing the pedigree data and identify Jun Qin and RunQing Yang contributed equally to this work.…”