Purification and molecular mass determination of a lipid transfer protein exuded from Vigna unguiculata seeds

Diz, Mariângela S.S.; Carvalho, André O.; Gomes, Valdirene Moreira

doi:10.1590/s1677-04202003000300007

Cited by 9 publications

(11 citation statements)

References 25 publications

(24 reference statements)

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“…These results suggest the state of an intense outflow of proteins from the seeds during the very beginning of germination. The release of antimicrobial proteins from seeds during germination has already been described on the literature (Terras et al 1995;Diz et al 2003). Furthermore it has been described that some antimicrobial peptides, such as LTP, are released by dipping the plant tissues in extraction buffer (Molina and Garcia-Olmedo 1993) and, in cell cultures are also secreted to the growth medium (Sterk et al 1991).…”

Section: Biological Effects Of Exuded Proteins On Fungi Growthmentioning

confidence: 99%

“…Furthermore it has been described that some antimicrobial peptides, such as LTP, are released by dipping the plant tissues in extraction buffer (Molina and Garcia-Olmedo 1993) and, in cell cultures are also secreted to the growth medium (Sterk et al 1991). We have previously characterized a LTP exuded from imbibed cowpea seeds (Diz et al 2003).…”

Section: Biological Effects Of Exuded Proteins On Fungi Growthmentioning

confidence: 99%

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Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

et al. 2006

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Plants exude a variety of substances through their roots, germinating seeds and aerial parts. Some of these released compounds seem to have an inhibitory effect against pathogens. The aim of this work was to investigate and identify antifungal proteins present in exudates from imbibed cowpea seeds (Vigna unguiculata (L.) Walp). The obtained exudation was analyzed in regard to specific protein activities by enzymatic or immunological assays for plant defense proteins, from 4 h to 48 h of seed imbibition. Our results show that cowpea seeds exudates present several defense related proteins characterized as b-1,3-glucanases, cystatins, vicilins and lipid transfer proteins (LTPs), as well as a storage vacuole membrane a-TIP protein, since the very first hours of imbibition. These exudates also have an ''in vitro'' inhibitory effect on the growth of the fungus Fusarium oxysporum f. sp. phaseoli. Our results suggest that seed exudates should promote seed protection from soil pathogens.

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Section: Biological Effects Of Exuded Proteins On Fungi Growthmentioning

confidence: 99%

Section: Biological Effects Of Exuded Proteins On Fungi Growthmentioning

confidence: 99%

Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

et al. 2006

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“…Until now, they have been identified and purified from seeds of many plant species 15,18,34,43,50,92,124,150 , including the most economically important cereals, i.e. maize 36 , wheat 39 , rice 157 and barley 143 .…”

Section: Structure Of Barley Grain Ns-ltp1mentioning

confidence: 99%

Barley Grain Non-specific Lipid-Transfer Proteins (ns-LTPs) in Beer Production and Quality

Gorjanović

2007

Journal of the Institute of Brewing

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Plant non-specific lipid-transfer proteins (ns-LTPs) are known for their ability to transfer various lipids between membranes in vitro. These ubiquitous basic proteins, that all share alpha structure stabilized by four disulphide bridges, are characterized by the presence of a hydrophobic cavity able to accommodate lipid molecules. According to molecular mass, this multigene family is subdivided into two subfamilies, ns-LTP1 (9 kDa) and ns-LTP2 (7 kDa); both located in the aleurone layer of the cereal grain endosperm. Ns-LTP1 is a prominent protein in barley grain, malt and beer. Numerous studies performed on its structure and function confirm its important role in grain protection, as well as brewing technology. As the major beer protein crucial for many aspects of brewing, ns-LTP1 can affect beer production and quality. Comparatively, there is less data available on the less abundant ns-LTP2. In this review, the focus is on recent progress on the structure, biological and technological function of barley grain ns-LTP1 and ns-LTP2, with an emphasis on their importance in brewing.

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“…Locating seed proteins and their release during the inhibition process can contribute to the control of seed-and soil-borne fungal diseases. Defensive proteins, therefore, enhance the chances of seedling survival and plant reproduction (Diz et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

Praxedes¹,

Zerlin²,

Dias³

et al. 2011

Braz. J. Biol.

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A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S)FS(P), showed growth inhibitory activity of filamentous fungi Aspergillus niger, Cladosporium cladosporioides, Colletotrichum gloeosporioides and Fusarium solani.Keywords: plant defence, antimicrobial protein, filamentous fungi, seed protein. Uma nova proteína antifúngica obtida de sementes de Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae) ResumoUma nova proteína com atividade antifúngica, com massa molar de cerca de 50 kDa, foi purificada de sementes de Sesbania virgata (Cav.) Pers. utilizando precipitação com sulfato de amônia, filtração em gel em coluna de Sephadex G-75 Superfine (Sigma) e cromatografia líquida de alta eficiência em fase reversa (coluna C8). A proteína purificada foi designada FP1-A, com a sequência N-terminal AMVHSPGG(S)FS(P), apresentando atividade inibitória do crescimento dos fungos filamentosos Aspergillus niger, Cladosporium cladosporioides, Colletotrichum gloeosporioides e Fusarium solani.Palavras-chave: mecanismo de defesa em planta, proteína antimicrobiana, fungos filamentosos, proteína de semente.

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Purification and molecular mass determination of a lipid transfer protein exuded from Vigna unguiculata seeds

Cited by 9 publications

References 25 publications

Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

Barley Grain Non-specific Lipid-Transfer Proteins (ns-LTPs) in Beer Production and Quality

A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

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