Leaf anatomy and carbon isotope composition in Coffea species related to photosynthetic pathway

Abstract

Possible presence of vascular bundle sheath cells and its relation to photosynthetic pathway, leaf anatomy and carbon isotope composition (delta13C) were examined in six species of genus Coffea: C. arabica (cvs. Catuaí Vermelho, Mundo Novo, Bourbon Vermelho and Icatu Amarelo), C. canephora (cvs. Apoatã and Guarini), C. liberica, C. dewevrei, C. salvatrix and C. stenophylla. In all genotypes, the vascular bundle was surrounded by a layer of cells with numerous chloroplasts in a centrifugal position. Visually no differences could be seen between the spongy parenchyma cells and the bundle sheath cells, neither in size nor in chloroplast number. The leaf delta13C values ranged between a maximum of - 26.2 ‰ in C. salvatrix and a minimum of -29.7 ‰ in C. liberica. A strong correlation (r = 0.972, p = 0.001) between delta13C and anatomical characteristics was observed in coffee species. C. salvatrix exhibited the highest delta13C values and the most compact mesophyll, with more palisade and spongy parenchyma cells in contact with the vascular bundle sheath.

Impact of drought associated with high temperatures on Coffea canephora plantations: a case study in Espírito Santo State, Brazil

“…A study of Rodríguez-López et al 45 with genotypes widely cultivated in state of Espírito Santo (clones 03 and 120) showed that saturating irradiance for leaf photosynthesis of conilon coffee at full sun is between 522–612 µmol photons m −2 s −1 . But, under Brazilian conditions, where coffee is commonly cultivated without shading, the plants must endure irradiance as high as 2000 µmol m −2 s −1 around midday 45 , 46 . This means that irradiance available for conilon coffee in these locations is around 3.5 times the irradiance saturation point, a fact that further contributes to exacerbate the negative effects of drought and heat.…”

Altitudinal variation of leaf carbon isotope for Dendrosenecio keniensis and Lobelia gregoriana in Mount Kenya alpine zone

“…Rada et al (1987) conducted this on Espeletia schultzii a composite species from South America and a found a decline in mesophyll, epidermis and cell size with increase in altitude. These findings are echoed by Carelli et al (2003) who found the lowest values of leaf δ 13 C in C . dewevrei , a C3 plant, with a decline in mesophyll cells.…”

Application of Stable Isotope Analysis for Detecting the Geographical Origin of the Greek Currants “Vostizza”: A Preliminary Study

“…Thus, more than four out of five samples can be correctly classified. The difference of the Corinthian currants species tested might also contribute to their variation in terms of of δ 13 C, as it has been proven that different coffee species varied in δ 13 C [ 38 ]. In a similar study, Li et al [ 39 ] showed that carbon isotope ratio could be used to identify the geographical origins of Schisandra fruits in China.…”