Positive, negative and marker-free strategies for transgenic plant selection

Aragão, F. J. L.; Brasileiro, Ana Cristina Miranda

doi:10.1590/s1677-04202002000100001

Cited by 18 publications

(8 citation statements)

References 74 publications

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“…In bacteria, kanamycin and related aminoglycoside antibiotics bind to rRNA in the small ribosomal subunit [4]. These antibiotics presumably act similarly on the prokaryotic-like ribosomes in the organelles of plant cells [5]. Until now, however, there was no information to our knowledge about how kanamycin is imported into either chloroplasts or mitochondria.…”

Section: Technical Reportmentioning

confidence: 99%

A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter

Aufsatz

Nehlin

Voronin

et al. 2009

Biotechnology Journal

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The use of bacterial antibiotic resistance markers in transgenic plants raises concerns about horizontal gene transfer to soil bacteria. We report here that kanamycin resistance in Arabidopsis thaliana can be achieved by silencing an endogenous gene encoding a putative chloroplast transporter, which presumably imports kanamycin into chloroplasts to interfere with ribosomal RNA. Homologs of the transporter exist in other plant species, suggesting this strategy may be generally useful for selecting transformed plant cells.

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Section: Technical Reportmentioning

confidence: 99%

A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter

Aufsatz

Nehlin

Voronin

et al. 2009

Biotechnology Journal

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“…Generation of transgenic plants free of these markers is a current challenge for biotechnology (Aragão and Brasileiro 2002). Consequently, several strategies to remove selective marker genes have been developed, such as co-transformation, multi-autotransformation (MAT), intragenomic relocation via transposable elements and site-specific recombination (Aragão et al 1996, Vergunst and Hooykaas 1999, Ebinuma and Komamine 2001, Pavingerová et al 2001, Raizada et al 2001.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Radiation as a tool to remove selective marker genes from transgenic soybean plants

Tinoco¹,

Vianna²,

Abud³

et al. 2006

Biol. plant.

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The present study evaluated the use of γ-radiation to physically remove selective marker genes previously introduced into the soybean genome. Homozygous seeds from a transgenic soybean line carrying the gus and ahas transgenes were irradiated with γ-rays. Six plants presenting a deleted gus gene were analyzed by Southern blot to confirm removal of both ahas and gus genes. Line 1A presented an absence of the gus gene cassette and presence of the ahas gene cassette.

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“…In the MAT system, the ipt gene is then eliminated via a linked Ac/Ds transposition as described above and marker-free, normal plants are recovered. The frequency of recovery is low but new systems hold promise to increase the efficiency (Aragão and Brasileiro, 2002). …”

Section: The Potential Of Marker-free Technologies In Transgenic Cassmentioning

confidence: 99%

Biosafety considerations for selectable and scorable markers used in cassava (Manihot esculentaCrantz) biotechnology

Petersen¹,

Umbeck²,

Hokanson

et al. 2005

Environ. Biosafety Res.

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Cassava is an important subsistence crop grown only in the tropics, and represents a major source of calories for many people in developing countries. Improvements in the areas of resistance to insects and viral diseases, enhanced nutritional qualities, reduced cyanogenic content and modified starch characteristics are urgently needed. Traditional breeding is hampered by the nature of the crop, which has a high degree of heterozygosity, irregular flowering, and poor seed set. Biotechnology has the potential to enhance crop improvement efforts, and genetic engineering techniques for cassava have thus been developed over the past decade. Selectable and scorable markers are critical to efficient transformation technology, and must be evaluated for biosafety, as well as efficiency and cost-effectiveness. In order to facilitate research planning and regulatory submission, the literature on biosafety aspects of the selectable and scorable markers currently used in cassava biotechnology is surveyed. The source, mode of action and current use of each marker gene is described. The potential for toxicity, allergenicity, pleiotropic effects, horizontal gene transfer, and the impact of these on food or feed safety and environmental safety is evaluated. Based on extensive information, the selectable marker genes nptII, hpt, bar/pat, and manA, and the scorable marker gene uidA, all have little risk in terms of biosafety. These appear to represent the safest options for use in cassava biotechnology available at this time.

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Positive, negative and marker-free strategies for transgenic plant selection

Cited by 18 publications

References 74 publications

A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter

A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter

Radiation as a tool to remove selective marker genes from transgenic soybean plants

Biosafety considerations for selectable and scorable markers used in cassava (Manihot esculentaCrantz) biotechnology

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