Comparison between two methods of ionized calcium measurement in newborns

Basseto, Thaís P.; Azzalis, Ligia Ajaime; Pereira, Edimar Cristiano; Junqueira, Virgínia Berlanga Campos; Caputo, Luciana Zambelli; Fonseca, André; Feder, David; Fonseca, Fernando Luiz Affonso

doi:10.1590/s1676-24442013000500003

Cited by 1 publication

(1 citation statement)

References 6 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples were centrifuged at 3000 rpm for 10 min. Serum was stored at 4°C and were sent to the Laboratory of Centro Universitário FMABC for analysis of 25(OH)D, PTH (immunometric assays by electrochemiluminescence), 21,22 ionized calcium (colorimetric, calculated), phosphorus (colorimetric), 23 alkaline phosphatase (colorimetric kinetics), and hs-CRP (immunoturbidimetric assay). 24 The Roche kit ® , Brazil, with fully automated reading and Cobas 6000 equipment (Roche ® ) was used for all analyses.…”

Section: Laboratory Testsmentioning

confidence: 99%

Vitamin D deficiency in children and adolescents with food allergy

Pereira

Mendonça

Fonseca

et al. 2022

Allergol Immunopathol

View full text Add to dashboard Cite

Our objective was to describe the serum vitamin D concentrations of children and adolescents with food allergy (FA) and to verify the association between the number of food allergens involved, length of sun exposure, and nutritional status. Through a cross-sectional study, 79 patients with FA, from ages 2 to 15 years, were assessed and followed up in a reference out-patient clinic, in Sao Paulo, Brazil. Clinical and biochemical data were collected for analysis of 25(OH)D, calcium, phosphorus, phosphatase, parathyroid hormone (PTH), and high-sensitivity C-reactive protein (hs-CRP). The cut-off point used for vitamin D deficiency was 25(OH)D ≤ 20 ng/mL. Vitamin D deficiency was detected in 45.6% of patients with a median age of 6.9 years (Interquartile range [IQR] 4.7; 10.2). The median serum 25(OH)D concentration was 21.1 ng/ mL (IQR 17.8; 26.0). Multivariate linear regression was performed considering serum vitamin D level as a dependent variable. Allergy to multiple foods (inverse) and length of sun exposure (direct), but not nutritional status, were independently associated with serum 25(OH)D levels (P = 0.034 and P = 0.014, respectively). Patients with cow’s milk allergy also showed lower vitamin D concentrations in comparison with other FA (19.1 ng/mL [IQR 16.6; 24.4] vs 22.2 ng/mL [IQR 18.1; 27.1] [P = 0.056]). Vitamin D deficiency affected about half of individuals with FA. Multiple food allergy was associated with lower vitamin D concentrations, reinforcing the importance of monitoring vitamin D status in patients with FA.

show abstract

Section: Laboratory Testsmentioning

confidence: 99%