Perda de amostras em tissue microarray: comparação entre técnicas com uso de fita adesiva comercial, lâminas silanizadas pelo método tradicional ou por método modificado

Almeida, Joaquim; Costa, Henrique de Oliveira; Lima, Flávio de Oliveira; Pinheiro, Nathanael; Oshima, Celina Tizuko Fujiyama; Gomes, Thiago Simão; Franco, Marcello

doi:10.1590/s1676-24442008000500008

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Using paraffin blocks (biopsies of lymph nodes or tumor masses), we constructed a tissue microarray (TMA) [ 10 ] using Beecher Instruments equipment (Estigen, Tartu, Estonia). Each case in the pilot study (n=71) was evaluated by an experienced pathologist (A.C.A.)…”

Section: Methodsmentioning

confidence: 99%

A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly

et al. 2014

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Currently, there is no characteristic microRNA (miRNA) expression pattern in Epstein-Barr virus+ diffuse large B-cell lymphoma of the elderly (EBV+DLBCLe). This study aims to characterize a signature profile and identify miRNAs that can be used as biomarkers and alternative therapeutic targets for EBV+DLBCLe. Seventy-one DLBCL patients aged 50 years and older were included and four EBV+ and four EBV– samples were analyzed in two miRNA array platforms (pilot study). A larger multicenter cohort (29 EBV+DLBCLe and 65 EBV–DLBCL patients) was used to validate the results by real-time polymerase chain reaction. In the pilot study, 9% of DLBCL were EBV+DLBCLe by in situ hybridization. In multicenter study, EBV+DLBCLe group showed a predominance of non-germinal center B-cell origin. Overall survival duration of EBV+DLBCLe was significantly inferior to that of EBV–DLBCL patients. We found 10 deregulated miRNAs in the two groups, but only seven were statistically different. We confirmed overexpression of hsa-miR-126, hsa-miR-146a, hsa-miR-146b, hsa-miR-150, and hsa-miR-222 and underexpression of hsa-miR-151 in EBV+DLBCLe cases compared to EBV–DLBCL cases. Hsa-miR-146b and hsa-miR-222 showed high specificity for identifying EBV+DLBCLe. The present study proposed a miRNA signature for EBV+DLBCLe and our findings suggest that hsa-miR-146b and hsa-miR-222 could be biomarkers and therapeutic targets.

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Section: Methodsmentioning

confidence: 99%

A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly

et al. 2014

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“…To ensure accuracy, a highly expressed breast tumor was used as a positive control, and a negative control was obtained by omitting the primary antibody. 13,16,17…”

Section: Immunohistochemistrymentioning

confidence: 99%

Trop-2 Is Highly Expressed in Early Luminal-Like Breast Cancer

Coelho,

Ruppenthal,

Graudenz

2023

J Histochem Cytochem.

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Trop-2, a transmembrane glycoprotein, has been identified in human epithelial cells as a contributor to tumor growth and unfavorable prognosis in breast cancer (BC). Our study aimed to assess the expression of Trop-2 protein via immunohistochemistry (IHC) and correlate it with clinicopathological features in early luminal-like BC. We conducted a cross-sectional study evaluating Trop-2 protein expression in tissue microarrays using IHC. The expression was evaluated by the H-score and the following categorization was used: H-Score 0 to <100 as low, H-Score 100 to 200 as intermediate, and H-Score >200 to 300 as high. The study included 84 patients with a median age of 57, of whom 70% had invasive ductal carcinomas, 75% were classified as T2, and 47.6% had no affected lymph nodes. Trop-2 expression was high in 56% of patients and intermediate in 38%. None of the patients had an H-Score of zero. No correlation was observed between Trop-2 expression and clinicopathological features, including age, histological subtype, grade, Ki67, tumor size, nodal status, lymphovascular invasion, tumor subtype, and pathological staging. We demonstrated that Trop-2 is highly expressed in early luminal-like BC and is not influenced by clinicopathological features.

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Perda de amostras em tissue microarray: comparação entre técnicas com uso de fita adesiva comercial, lâminas silanizadas pelo método tradicional ou por método modificado

Cited by 2 publications

References 16 publications

A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly

A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly

Trop-2 Is Highly Expressed in Early Luminal-Like Breast Cancer

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