Análise comparativa da proliferação celular entre carcinomas de células escamosas orais HPV-positivos e HPV-negativos

Rocha, Danielle Albuquerque Pires; Souza, Lélia Batista de; Pinto, Leão Pereira

doi:10.1590/s1676-24442007000400009

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…The high oncogenic risk viruses are: HPV-16, HPV-18, HPV-31, HPV-33, HPV-45, HPV-51, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV-68, HPV-73 and the HPV-82. These are associated with atypical hyperplasia and oral carcinoma 17 , 18 . In these cases, HPV-16 has been more frequently found 19 , 20 , 21 .…”

Section: Introductionmentioning

confidence: 99%

Infecção oral pelo HPV em mulheres com lesão escamosa de colo uterino no sistema prisional da cidade de São Paulo, Brasil

Zonta

Monteiro

Santos

et al. 2012

Braz. j. otorhinolaryngol.

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Carci noma of the head and neck is the 6 th cause of death by cancer in the world. In recent decades the human papillomavirus (HPV) has been implicated in the etiology of this disease Objective: To characterize the types of HPV detected in the oral mucosa in women with cytological abnormalities suggesting intraepithelial squamous lesions in the uterine cervix.Methods: four-hundred-nine cervical-vaginal and oral pap-smears of women interned in a Female Prison in São Paulo were examined. The relationship between cervical and oral lesion was analyzed by PCR/RFLP and DNA sequencing.Results: Of 27 (6.67%) specimens showing cervical cytological abnormalities suggesting LSIL and HSIL, 22 (81.48%) had oncogenic high-risk HPV infection, of which HPV 59 was the most prevalent. Three (11.1%) samples showed cytological changes suggesting mild dysplasia in the oral cavity. Conclusion:Our study suggests an association between carcinoma of the oral cavity and HPV infection, regardless of the virus type. Braz J Otorhinolaryngol. 2012;78(2):66-72. ORIGINAL ARTICLE BJORL

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Section: Introductionmentioning

confidence: 99%

Infecção oral pelo HPV em mulheres com lesão escamosa de colo uterino no sistema prisional da cidade de São Paulo, Brasil

Zonta

Monteiro

Santos

et al. 2012

Braz. j. otorhinolaryngol.

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“…As técnicas imuno-histoquímicas, entre outras indicações, contribuem para a graduação e para estabelecer o prognóstico das neoplasias (9,14) . O Ki-67 tem sido muito usado como marcador de proliferação e fator prognóstico de neoplasias malignas.…”

Section: Introductionunclassified

Histomorfometria e índice proliferativo (Ki-67) no carcinoma escamocelular in situ de pregas vocais

Mendonça¹,

Miranda²,

Telles³

et al. 2012

J. Bras. Patol. Med. Lab.

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Introdução:As displasias da laringe são precursoras do carcinoma escamocelular invasivo e constituem lesões pouco comuns, pois a maior parte dos casos é detectada como carcinoma invasor. Objetivo: Caracterizar as displasias acentuadas/carcinoma in situ da prega vocal quanto a área do epitélio, diâmetro dos núcleos e índice proliferativo, comparando esses dados com os obtidos no epitélio escamoso normal e de transição. Material e método: Entre as 1.400 biópsias e peças cirúrgicas de lesões intraepiteliais e carcinomas invasivos de laringe (1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005)(2006), foram selecionados cinco casos (0,35%) pareados com dois controles de epitélio normal e com a idade; todos foram submetidos a análise morfométrica e imuno--histoquímica (Ki-67). Resultados: Comparando-se o epitélio displásico com o normal e de transição observou-se maior área e diâmetro nuclear no epitélio displásico; não houve diferenças significativas no diâmetro dos núcleos por camada entre o epitélio displásico e de transição; e o índice proliferativo foi maior no epitélio displásico com núcleos corados em todas camadas e menor com núcleos limitados a camada basal e parabasal no epitélio normal e de transição. Conclusão: verifica-se maior área no epitélio displásico. O diâmetro dos núcleos do epitélio displásico e do de transição são semelhantes, mas este apresenta núcleos uniformes em toda extensão em contraste com o pleomorfismo do epitélio displásico. O índice proliferativo (Ki-67) contribui para o diagnóstico diferencial das lesões escamosas da prega vocal, pois no carcinoma in situ/displasia acentuada observa-se maior número de núcleos marcados, os quais são vistos em todas as camadas no epitélio. resumo unitermos Laringe Carcinoma in situ Prega vocal Antígeno Ki-67abstract Introduction: Laryngeal dysplasias, precursors of squamous cell carcinomas, are uncommon lesions, inasmuch as most cases are diagnosed as invasive carcinomas. Objective: To characterize severe dysplasia/in situ carcinoma of the vocal cords by comparing the area of involved epithelium, nuclear diameter, and proliferative index with transition and normal squamous epithelia. Material and method: Among 1,400 surgical and biopsy specimens of laryngeal lesions (1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005)(2006), five cases (0.35%) were selected and compared according to age with two controls with normal epithelium. Furthermore, all of them were identified and submitted to morphometric and immunohistochemical analysis . Results: After comparing dysplastic with transition and normal squamous epithelia, it was observed an increased nuclear diameter in dysplastic epithelium. Additionally, there was no significant difference in nuclear diameter per layer between dysplastic and transition epithelia. Moreover, the proliferative index was higher in dysplastic epithelium with stained nuclei in all layers and lower in normal and transition epithelia with nuclei concentrated on the basal and parabasal layers. Con...

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Modification in CLIC4 Expression is Associated with P53, TGF-β, TNF-α and Myofibroblasts in Lip Carcinogenesis

et al. 2020

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Chloride intracellular channel-4 (CLIC4) is regulated by p53 and tumor necrosis factor-α (TNF-α), it is linked to the increase of transforming growth factor-β (TGF-β), and myofibroblastic differentiation in skin carcinogenesis. This study analyzed the immunoexpression of CLIC4, p53, TGF-β, TNF-α, and α-SMA in 50 actinic cheilitis (AC) and 50 lower lip squamous cell carcinoma (LLSCC). AC and LLSCC immunoexpression were categorized as score 1 (<5% positive cells), 2 (5-50%) or 3 (>50%). For CLIC4, nuclear and cytoplasmic immunostaining of epithelial cells was considered individually. For morphologic analysis, the World Health Organization criteria were used to epithelial dysplasia grade of ACs, and Bryne grading of malignancy system was applied for LLSCC. Higher nuclear CLIC4 (CLIC4n) and TGF-β were observed in ACs with low-risk of transformation, while cytoplasmic CLIC4 (CLIC4c), p53 and TNF-α were higher in the high-risk cases (p<0.05). In LLSCCs, CLIC4c was higher in cases with lymph node metastasis, advanced clinical stages, and histological high-grade malignancy. p53 expression was higher in high-grade LLSCCs, whereas TGF-β decreased as the clinical stage and morphological grade progressed (p<0.05). ACs showed an increased expression of CLIC4n and TGF-β, while CLIC4c and α-SMA were higher in LLSCCs (p<0.0001). Both lesions showed negative correlation between CLIC4n and CLIC4c, while in LLSCCs, negative correlation was also verified between CLIC4c and p53, as well as CLIC4c and TGF-β (p<0.05). Change of CLIC4 from the nucleus to cytoplasm and alterations in p53, TGF-β, TNF-α, and α-SMA expression are involved in lip carcinogenesis.

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Análise comparativa da proliferação celular entre carcinomas de células escamosas orais HPV-positivos e HPV-negativos

Cited by 3 publications

References 27 publications

Infecção oral pelo HPV em mulheres com lesão escamosa de colo uterino no sistema prisional da cidade de São Paulo, Brasil

Infecção oral pelo HPV em mulheres com lesão escamosa de colo uterino no sistema prisional da cidade de São Paulo, Brasil

Histomorfometria e índice proliferativo (Ki-67) no carcinoma escamocelular in situ de pregas vocais

Modification in CLIC4 Expression is Associated with P53, TGF-β, TNF-α and Myofibroblasts in Lip Carcinogenesis

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