Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis

Melo-Júnior, Mário Ribeiro de; Araújo-Filho, Jorge Luiz Silva; Patu, Vasco José Ramos Malta; Machado, Marcos Cezar Feitosa de Paula; Beltrão, Eduardo Isidoro Carneiro; Carvalho, Luiz Bezerra de

doi:10.1590/s1676-24442006000600009

Cited by 12 publications

(10 citation statements)

References 30 publications

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“…8,18,19 In normal squamous cell epithelia, the glycophenotype is characterized by α2,6-linked sialic acid expression in epithelium cells of the basal layer and α2,3-linked sialic acid in basal as well as suprabasal layers. 19 …”

Section: Discussionmentioning

confidence: 99%

Expression patterns of α2,3-Sialyltransferase I and α2,6-Sialyltransferase I in human cutaneous epithelial lesions

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2013

Eur J Histochem

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Skin tumors have become one of the most common cancers in the world and their carcinogenesis is frequently associated with altered glycosylation patterns. The aberrant sialylation, a type of glycosylation, can mediate pathophysiological key events during various stages of tumor progression, including invasion and metastasis. Sialyltransferases play a key role in a variety of biological processes, including cell-cell communication, cell-matrix interaction, adhesion, and protein targeting. In this study, it was evaluated the expression of ST3Gal I and ST6Gal I in cutaneous epithelial lesions that include actinic keratosis (n=15), keratoacanthoma (n=9), squamous cell carcinoma (n=22) and basal cell carcinoma (n=28) in order to evaluate if sialyltransferases expression is different in premalignant and in malignant tumors. The expression of ST3Gal I was observed in actinic keratosis (53%), keratoacanthoma (78%), squamous cell carcinoma (73%) and basal cell carcinoma (32%) with statistic differences between basal cell carcinoma and keratoacanthoma (P=0.0239) and basal cell carcinoma and squamous cell carcinoma (P=0.0096); for ST6Gal I, cytoplasmic expression was noted in actinic keratosis (40%), heterogeneous and cytoplasmic expression was noted in keratoacanthoma (67%), squamous cell carcinoma (41%) and basal cell carcinoma (7%) with statistic differences between basal cell carcinoma and squamous cell carcinoma (P=0.0061) and basal cell carcinoma and keratoacanthoma (P=0.0008). In summary, our results showed that the high expression of ST3Gal I and ST6Gal I, in skin tumors, is associated with tumors with greater potential for invasion and metastasis, as in the case of squamous cell carcinoma, and this may be related to their behavior.

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Section: Discussionmentioning

confidence: 99%

Expression patterns of α2,3-Sialyltransferase I and α2,6-Sialyltransferase I in human cutaneous epithelial lesions

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2013

Eur J Histochem

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“…Alterations in the expression of glycophenotype between benign and malignant skin lesions have been previously reported through lectin histochemistry and computer image analysis [8]. It is worthwhile to register that they did not compare with normal epidermis and included trichoepithelioma and seborrheic keratosis that were not studied in this work.…”

Section: Discussionmentioning

confidence: 99%

“…These tumors are rarely fatal but are considered to be fast growing and if neglected may be locally and functionally destructive [18]. The diagnosis of skin neoplasias becomes inaccurate, in some cases, due to a variety of factors that affect the test accuracy, such as a huge spectrum of tumors and their variants and the lower differentiation [8]. …”

Section: Introductionmentioning

confidence: 99%

Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester

et al. 2013

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Background. Tumor cells show alterations in their glycosylation patterns when compared to normal cells. Lectins can be used to evaluate these glycocode changes. Chemiluminescence assay is an effective technique for quantitative analysis of proteins, nucleic acids, and carbohydrates due to its high sensitivity, specificity, and rapid testing. Objective. To use histochemiluminescence based on lectin conjugated to acridinium ester (AE) for the investigation of glycophenotype changes in cutaneous tumors. Methods. Concanavalin A (Con A), Peanut agglutinin (PNA), Ulex europaeus agglutinin-I (UEA-I), and Maackia amurensis agglutinin (MAA) were conjugated to acridinium ester. Biopsies of cutaneous tumors and normal skin were incubated with the lectins-AE, and chemiluminescence was quantified and expressed as Relative Light Units (RLU). Results. Actinic keratosis (AK), keratoacanthoma (KA), squamous cell carcinoma (SCC), and basal cell carcinoma (BCC) showed lower expression of α-D-glucose/mannose and α-L-fucose residues compared to normal tissue. Cutaneous tumors displayed higher expression of Gal-β(1-3)-GalNAc residues than normal tissue. AK and SCC exhibited higher expression of Neu5Ac-α(2,3)Gal residues than normal epidermis. KA and BCC showed equivalent RLU values compared to normal tissue. Conclusions. Lectin histochemiluminescence allowed quantitative assessment of the carbohydrate expression in cutaneous tissues, contributing to eliminate the subjectivity of conventional techniques used in the histopathological diagnosis.

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“…For instance, FITC has been conjugated to lectin for recognizing pterygium remnant after surgical excision (Díaz‐González et al, ). In our lab, lectins conjugated to different kinds of labels have been employed to analyzing the cancer glycophenotype in histochemistry (Brustein et al, ; Lima et al, ; Melo‐Júnior, Araújo‐Filho, Patú, Beltrão, & Carvalho, ; Santos et al, ; Silva et al, ). Lectins are structurally diverse carbohydrate‐binding proteins of non‐immune origin that agglutinate cells and recognize carbohydrates in oligosaccharides and glycoconjugates (Sharon, ).…”

Section: Introductionmentioning

confidence: 99%

Dimethyl‐2‐[(acridin‐9‐yl)methylidene]‐malonate as fluorescent probe for histochemical analysis

Almeida

Silva

Lima

et al. 2017

Microscopy Res & Technique

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Fluorescent compounds have been widely used for biomolecule labeling in cytochemistry and histochemistry analysis. Here, it is described the optical properties of dimethyl 2-[(acridin-9-yl)methylidene]-malonate (LPSF/IP-81), an acridine derivative. This compound was conjugated to Concanavalin A (Con A) lectin and applied as sugar probe in lectin histochemistry. Evaluation of luminescent properties showed that LPSF/IP-81 is photoluminescent with excitation at 360 nm and emission at 428 nm. Con A hemagglutinating activity and LPSF/IP81 photoluminescence were unaltered after conjugation. Circular dichroism of Con A-LPSF/IP81 conjugate showed the maintenance of the Con A structure. Lectin histochemistry with Con A-LPSF/IP81 conjugate demonstrated different pattern recognition studying normal, fibroadenoma, and invasive ductal carcinoma of human breast. These findings indicate that LPSF/IP-81 can be proposed as an alternative probe for histochemical analysis.

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Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis

Cited by 12 publications

References 30 publications

Expression patterns of α2,3-Sialyltransferase I and α2,6-Sialyltransferase I in human cutaneous epithelial lesions

Expression patterns of α2,3-Sialyltransferase I and α2,6-Sialyltransferase I in human cutaneous epithelial lesions

Glycophenotype Evaluation in Cutaneous Tumors Using Lectins Labeled with Acridinium Ester

Dimethyl‐2‐[(acridin‐9‐yl)methylidene]‐malonate as fluorescent probe for histochemical analysis

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