Determination of HPV DNA viral load by hybrid capture assay and its association with cytological findings

Tozetti, Inês Aparecida; Scapulatempo, Ilzia Doraci Lins; Levi, José Eduardo; Ferreira, Antônio Walter

doi:10.1590/s1676-24442006000600008

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Viral load, quantified by HCII, was classified into four groups, according to Lorincz et al 16 : from 0.1 to less than 10, from 10 to less than 100, from 100 to less than 1,000, and from 1,000 or more relative light units/positive control to group B (RLU/PCB). The data for histopathological diagnosis and viral load (Table 1) were provided by a previous study 17 .…”

Section: Subjectsmentioning

confidence: 99%

S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions

Cassandri

Tozetti

Fernandes

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Some human papillomavirus (HPV) types are involved in malignant processes in the cervical epithelium, with 99% of cases attributed to oncogenic HPV infection. This study aimed to detect S100, CD68, and major histocompatibility complex class II (MHC-II) molecules in cervical uterine epithelial samples in patients with high- and low-grade lesions induced by HPV. METHODS: Fifty-eight samples from patients who were confirmed positive or negative for high-risk oncogenic HPV DNA, had histopathological diagnosis of cervical intraepithelial neoplasia (CIN) of grades I, II, or III, or were negative for intraepithelial lesion or malignancy were subjected to immunohistochemistry reaction to S100 protein, CD68, and MHC-II (HLA-DR alpha chain). RESULTS: The presence of MHC-II predominated in samples exhibiting histopathological alterations (p < 0.05). S100 detection was more numerous in carcinoma samples (CIN III) (75%). Presence of this protein correlated significantly (p < 0.05) with histopathological findings and viral load. CONCLUSIONS: A small expression of CD68 was observed, which may be explained by the observation in our study having been made on random microscopic fields and not on specific areas. The findings, such as the presence of S100 protein and MHC-II expression in samples with histological alterations, could suggest that the immune system fails to control HPV replication at the early stages of infection. Further studies with larger prospective data are necessary to confirm this result.

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Section: Subjectsmentioning

confidence: 99%

S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions

Cassandri

Tozetti

Fernandes

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…Os dados relativos à carga viral e aos achados histopatológicos são provenientes de estudos anteriores 12 . 60mg%.…”

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Linfócitos CD4, CD8 e células NK no estroma da cérvice uterina de mulheres infectadas pelo papilomavírus humano

Alves

Tozetti

Gatto

et al. 2010

Rev. Soc. Bras. Med. Trop.

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RESUMOIntrodução: A resposta imune pode ser um elemento chave para a progressão ou remissão da infecção pelo papilomavírus humano (HPV) no estroma da cérvice uterina. Este estudo objetivou quantificar no estroma cervical a presença de linfócitos T CD4, CD8 e células NK, por imunohistoquímica, em lesões de alto e baixo grau em pacientes infectadas por HPV. Métodos: Utilizou-se 56 amostras de biópsia da estroma cervical, sendo 43 amostras positivas para DNA de HPV de alto risco oncogênico e com diagnóstico histopatológico de neoplasia intraepitelial cervical (NIC) de alto e baixo grau, ou negativa para lesão intraepitelial e malignidade (NILM), e 13 amostras de pacientes negativas para DNA de HPV com diagnóstico histopatológico NILM. Resultados: Maior quantidade de linfócitos T CD4 foi observada em amostras NIC II/III, carcinoma e NILM (p=0,04) e naquelas cuja carga viral esteve entre 10 e 1,000 RLU/PCB. O predomínio de linfócitos T CD8 ocorreu em maior proporção nas amostras NIC II/III (p=0,02) e em amostras com carga viral entre 100 e 1.000 RLU/PCB. As células NK prevaleceram nas amostras com lesões de baixo grau e com baixa carga viral. Conclusões: Este estudo comprovou que nas fases iniciais da infecção, onde não há ainda alterações celulares de alto grau, não temos a presença de células que possam desencadear a fase efetora da resposta imune. Palavras-chaves: Papilomavírus humano. Resposta imunológica. Imunohistoquímica. ABSTRACT Introduction:Immune response might be a key element regarding the progression or regression of human papillomavirus (HPV) infection in the stroma of the uterine cervix. This study aimed to quantify the presence of CD4 and CD8 T lymphocytes and NK cells in the cervical stroma, by means of immunohistochemistry, in high and low grade lesions in patients infected by HPV. Methods: Fifty-six biopsy samples from the uterine cervix were used. Fortythree samples were positive for oncogenic high-risk HPV DNA and had a histopathological diagnosis of high and low-grade cervical intraepithelial neoplasia (CIN) or negative for intraepithelial lesion and malignancy (NILM); while the other 13 samples were negative for HPV DNA with a histopathological diagnosis of NILM. Results: Higher quantities of CD4 T lymphocytes were observed in CIN II/III, carcinoma and NILM samples (p = 0.04) and in those in which the viral load was between 10 and 1.000 RLU/PCB. CD8 T lymphocytes were predominant in CIN II/III samples (p = 0.02) and also in samples with viral loads between 100 and 1,000 RLU/PCB. NK cells predominated in samples with low-grade lesions and low viral load. Conclusions: This study proved that in the initial stages of the infection, in which no high-grade cell abnormalities have yet occurred, no cells that might trigger the effector phase of the immune response.

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Genotyping of High Risk Human Papillomavirus (HPV) among cervical precancer and cancer patients

et al. 2015

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Determination of HPV DNA viral load by hybrid capture assay and its association with cytological findings

Cited by 3 publications

References 14 publications

S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions

S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions

Linfócitos CD4, CD8 e células NK no estroma da cérvice uterina de mulheres infectadas pelo papilomavírus humano

Genotyping of High Risk Human Papillomavirus (HPV) among cervical precancer and cancer patients

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