A simple automated procedure for thiol measurement in human serum samples

Costa, Carolina Marinho da; Santos, Rita C. C. dos; Lima, Émerson Silva

doi:10.1590/s1676-24442006000500006

Cited by 112 publications

(82 citation statements)

References 24 publications

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“…The concentration of total sulfhydryl (SH) groups was measured according to Ellman (1959) and Hu (1994) adapted to an autoanalyzer (Cobas Mira Plus ® ) as described by Costa et al (2006). The total antioxidant status (TOS) of blood plasma was measured with 2, 2-azinobis 3-ethylbenzothiazoline-6-sulfonate (Erel 2004).…”

Section: Markers Of Os and Antioxidant Status -mentioning

confidence: 99%

The role of platelet and plasma markers of antioxidant status and oxidative stress in thrombocytopenia among patients with vivax malaria

Araujo

Lacerda

Abdalla

et al. 2008

Mem. Inst. Oswaldo Cruz

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Section: Markers Of Os and Antioxidant Status -mentioning

confidence: 99%

The role of platelet and plasma markers of antioxidant status and oxidative stress in thrombocytopenia among patients with vivax malaria

Araujo

Lacerda

Abdalla

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…The total reduced glutathione (GSH) was determined in the liver as described by Sedlack & Lindsay [22] and the serum GSH was determined by the method of Costa et al [23].…”

Section: Biochemistries Determinationsmentioning

confidence: 99%

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Leonardi-Carvalho¹,

Zucoloto²,

Ovídio³

et al. 2015

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Abstract:The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p<0.05). The hepatic cholesterol concentration was highest in the HF group and the serum total cholesterol concentration was highest in the HPF group (p<0.05). The macrovesicular steatosis was predominant in the HF group, with ballooning hepatocytes and Mallory bodies and in the HPF group predominant microvesicular steatosis was found, without ballooning hepatocytes and Mallory bodies. An increase in TBARS and a decrease in Vitamin E in the HF and HPF groups (p<0.05) was also found. The HF group showed the highest acid oleic deposit in the liver, followed by the HPF group. The SFA hepatic concentration was similar among the groups (p>0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.

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“…Here, this method was adapted to an automated biochemistry analyzer (Advia 1800; Siemens, Germany) [17].…”

Section: Measurement Of Total Thiol Activitiesmentioning

confidence: 99%

Evaluation of Oxidative Stress Status and Antioxidant Capacity in Patients with Localized Prostate Cancer and Benign Prostatic Hyperplasia

Aldemir¹

2015

Ann Clin Anal Med

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ÖzetAmaç: Bu çalışma, lokalize prostat kanseri (PCa) ve benign prostat hiperplazili (BPH) hastalarda oksidatif stres durumu ve antioksidan kapasitenin değer-lendirilmesi amacıyla yapılmıştır. Gereç ve Yöntem: Bu prospektif çalışmaya, tamamı histolojik tanılı 24 lokalize PCa ve 36 BPH'lı erkek hasta dahil edilmiştir. Lokalize PCa ve BPH tanılı hastaların sabah açlık kan örneklerinden, total oksidan durum (TOS), total antioksidan kapasite (TAC), paraoxonase1 (PON1), arylesterase ve total thiol düzeyleri araştırılmış ve karşılaştırılmış-tır. Bulgular: Hastaların ortalama yaşı BPH grubunda 67 ± 12 yıl, localize PCa grubunda ise 68 ± 8 yıl idi (p>0.05). Elde edilen verilere göre, tüm oksidatif stress parametreleri ve antioksidan kapasite değerleri açısından iki grup arasında istatistiksel anlamlı fark olmadığı tespit edilmiştir (tüm parametreler için p>0.05). Tartışma: Çalışmamızda elde ettiğimiz sonuçlara dayanarak, serumda çalışılan bu parametrelerin localize PCa ve BPH'lı hastaların ayırıcı tanısında bir belirteç olarak kullanılamayacağını göstermiştir. Anahtar KelimelerLokalize Prostat Kanseri; Oksidatif Stres; Antioksidan Kapasite Abstract Aim: The aim of this study was to evaluate the oxidative stress status and antioxidant capacity in patients with localized prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Material and Method: The prospective study consisting 24 men with PCa and 36 men with BPH. PCa or BPH were diagnosed histologically. Blood samples were obtained following an overnight fasting state. We evaluated and compared the total oxidant status (TOS), total antioxidant capacity (TAC), paraoxonase1 (PON1), arylesterase and total thiol levels of patients with PCa and BPH. Results: The mean age of BPH group was 67 ± 12 years and PCa group was 63 ± 8 years (p>0.05). No statistically significant differences were detected between the groups in terms of oxidative stress parameters and antioxidant capacity measured in the serum of patients including PON1, TOS, TAC, arylesterase and thiols levels (p>0.05 for all parameters). Discussion: Our results demonstrated that evaluation of these parameters in the serum of BPH and localized PCa patients may not be used as a marker to discriminate between these diseases.

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A simple automated procedure for thiol measurement in human serum samples

Cited by 112 publications

References 24 publications

The role of platelet and plasma markers of antioxidant status and oxidative stress in thrombocytopenia among patients with vivax malaria

The role of platelet and plasma markers of antioxidant status and oxidative stress in thrombocytopenia among patients with vivax malaria

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Evaluation of Oxidative Stress Status and Antioxidant Capacity in Patients with Localized Prostate Cancer and Benign Prostatic Hyperplasia

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