Metalo-beta-lactamases

Mendes, Rodrigo E.; Castanheira, Mariana; Pignatari, Antônio Carlos Campos; Gales, Ana Cristina

doi:10.1590/s1676-24442006000200007

Cited by 36 publications

(50 citation statements)

References 60 publications

(87 reference statements)

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“…Table 1 presents the oligonucleotide and amplicon sequences used in the PCRs (10) (12) (13) (14) . The genotypic analysis of the isolates was performed using repetitive element (rep)-PCRs and the DiversiLab® System (bioMérieux).…”

Section: Molecular Detection Of the Bla-variant Genes And Molecular Tmentioning

confidence: 99%

“…This resistance mechanism has spread across hospitals because of the frequent use of carbapenems, which were considered the only effective antibiotic against P. aeruginosa infections (5) (8) . In Brazil, several MBL-producing multidrugresistant strains have been isolated, and blaSPM-1, which was initially detected in São Paulo, is the most prevalent gene (6) (9) (10) .…”

Section: Introductionmentioning

confidence: 99%

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Clinical and microbiological features of infections caused by Pseudomonas aeruginosa in patients hospitalized in intensive care units

Matos

Conceição

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:The spread of multidrug-resistant Pseudomonas aeruginosa in Brazilian hospitals has greatly impacted upon the morbidity and mortality of individuals in intensive care units. Given the lack of information regarding the dynamics of multidrug resistance in northern Brazil, we analyzed the clinical and microbiological features of nosocomial infections caused by P. aeruginosa. Methods: Between January 2010 and March 2012, we conducted a retrospective cohort study of P. aeruginosa isolates from 54 patients who were hospitalized in intensive care units. The clinical and epidemiologic variables were analyzed, including the patients' demographic data and comorbidities, and the lengths of the intensive care unit stays, the classification of the infections as nosocomial, the use of invasive procedures, antimicrobial therapy, and the patients' outcomes. We undertook susceptibility tests, molecular detection of the metallo-β-lactamase genes, and genotypic analyses of the isolates using the repetitive element-polymerase chain reaction. Results: Multidrug resistance occurred most frequently among isolates from adults who had been hospitalized for an average of 87.1 days. The use of mechanical ventilation and urinary catheters were risk factors for infection. The four isolates that harbored the blaSPM-1-like gene showed >95% genetic similarity. Conclusions: This study's findings show that P. aeruginosa has a high death rate, and that inadequate treatment and invasive procedures are risk factors for infection. This is the first report describing the detection of the blaSPM-1-like gene in northern Brazil. These results highlight the need for better monitoring and a greater understanding of nosocomial infections and their public health impacts.

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Section: Molecular Detection Of the Bla-variant Genes And Molecular Tmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clinical and microbiological features of infections caused by Pseudomonas aeruginosa in patients hospitalized in intensive care units

Matos

Conceição

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…Coco-bacilli and Gramnegative bacteria, such as Pseudomonas aeruginosa and Acinetobacter sp. respectively, are extremely important pathogens that cause serious infections in different parts of the human body and may lead to patient death [1][2][3] .…”

Section: Palavras-chaves: Mβl Resistência Bacteriana Pseudomonas Aementioning

confidence: 99%

Ocorrência e perfil de sensibilidade a antimicrobianos em Pseudomonas aeruginosa e Acinetobacter sp. em um hospital terciário, no sul do Brasil

Machado

Lago

Fuentefria

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Occurrence and the susceptibility to antimicrobial agents inABSTRACT Introduction: The main mechanism of emerging resistance in Pseudomonas aeruginosa and Acinetobacter sp. isolates is the production of metallo-β-lactamases (MβLs). MβLs are enzymes capable of hydrolyzing cephalosporins, penicillins and carbapenems, but not monobactams (aztreonam), which are often used as antimicrobial therapy to treat nosocomial infections. Methods: An observational descriptive and retrospective study was designed to assess the frequency of MβLs among strains of P. aeruginosa and Acinetobacter sp. obtained from a tertiary hospital in southern Brazil. Results: MβL production was observed in 77.6% (n = 173/223) for P. aeruginosa isolates and 22.4% (n = 50/223) of Acinetobacter sp. isolates. The Acinetobacter sp. isolates showed 92.8% sensitivity to amikacin and P. aeruginosa isolates showed 58.9% sensitivity to aztreonam. Conclusions: The MβL indices determined confirm the global concern with this mechanism of resistance.

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“…Atualmente apenas a enzima SPM-1 é codificada por um gene localizado em plasmídeo de aproximadamente 180 kb , as demais enzimas MßL ficam em integrons da classe 1 (Mendes et al, 2006;Toleman et al, 2002).…”

Section: Genes Que Codificam a Mßl Foram Encontrados Inseridos Em Estunclassified

Alternativas terapêuticas para o tratamento de infecções por Pseudomonas aeruginosa multirresistentes endêmicas no Brasil.

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Metalo-beta-lactamases

Cited by 36 publications

References 60 publications

Clinical and microbiological features of infections caused by Pseudomonas aeruginosa in patients hospitalized in intensive care units

Clinical and microbiological features of infections caused by Pseudomonas aeruginosa in patients hospitalized in intensive care units

Ocorrência e perfil de sensibilidade a antimicrobianos em Pseudomonas aeruginosa e Acinetobacter sp. em um hospital terciário, no sul do Brasil

Alternativas terapêuticas para o tratamento de infecções por Pseudomonas aeruginosa multirresistentes endêmicas no Brasil.

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