Técnica histoquímica aplicada ao tecido ósseo desmineralizado e parafinado para o estudo do osteócito e suas conexões

Ocarino, Natália de Melo; Gomes, M.G.; Melo, Eliane Gonçalves de; Serákides, Rogéria

doi:10.1590/s1676-24442006000100008

Cited by 4 publications

(2 citation statements)

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“…Sections were subjected to the Silver impregnation method to identify osteocyte cytoplasmic processes, as previously described [37]. Briefly, deparaffinized and hydrated sections were immersed in milliQ water for 10 min.…”

Section: Morphological Analysis Of Osteocyte Cytoplasmic Processes Bymentioning

confidence: 99%

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Florencio‐Silva

Sasso

Sasso-Cerri

et al. 2018

Biomedicine & Pharmacotherapy

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Estrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3α and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3α-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3α-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.

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Section: Morphological Analysis Of Osteocyte Cytoplasmic Processes Bymentioning

confidence: 99%

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Florencio‐Silva

Sasso

Sasso-Cerri

et al. 2018

Biomedicine & Pharmacotherapy

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“…Dentre as funções mecânicas, os osteócitos atuam como células sensoriomecânicas, transformando os estímulos mecânicos recebidos sobre o esqueleto, em sinais bioquímicos de ativação da remodelação óssea e deposição mineral (Ocarino et al, 2006;Yao, 2009 Por meio de ensaios destrutivos específicos tem sido possível determinar algumas propriedades biomecânicas de um material biológico como o osso, tanto na forma de corpo de prova quanto na sua estrutura íntegra, sendo estas, denominadas propriedades biomecânicas intrínsecas e extrínsecas Burr, 1993;Rizzoli, 2003).…”

Section: Revisão De Literaturaunclassified

Repercussões minerais ósseas no alcoolismo crônico associado à deficiência hormonal em ratos

Lodi¹

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Heat Generated by Dental Implant Drills During Osteotomy—A Review

Mishra¹,

Chowdhary²

2014

J Indian Prosthodont Soc

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The relationship between heat generated and implant drilling osteotomy is multifactorial in nature and its complexity has not been fully studied. Lack of scientific knowledge regarding this issue still exists. Further studies should be conducted to determine the various factors which generate less heat while osteotomy such as ideal ratio of force and speed in vivo, exact time to replace a drill, ideal drill design, irrigation system, drill-bone contact area.

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Técnica histoquímica aplicada ao tecido ósseo desmineralizado e parafinado para o estudo do osteócito e suas conexões

Cited by 4 publications

References 4 publications

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Repercussões minerais ósseas no alcoolismo crônico associado à deficiência hormonal em ratos

Heat Generated by Dental Implant Drills During Osteotomy—A Review

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