Regiões organizadoras nucleolares argirofílicas no sarcoma sinovial

Lima, Flávio de Oliveira; Catarino, Regina Maria; Oshima, Celina Tizuko Fujiyama; Figueiredo, Marco Túllio de Assis

doi:10.1590/s1676-24442005000500010

Cited by 2 publications

(2 citation statements)

References 16 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The AgNOR index could be used as an additional option for cases where IHC for Ki67 antigen could not be used, or in combination with the Ki67 result, as it will be discussed later. This is justified by the fact that the retrospective studies that used the AgNOR technique, possibly without knowledge of sample fixation time, did not mention the influence of fixation time on the results obtained (Rech et al 2004, Lima et al 2005.…”

Section: Discussionmentioning

confidence: 99%

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

et al. 2019

View full text Add to dashboard Cite

Due to the high prevalence of mast cell tumors (MCTs) in the diagnostic routine, several factors, especially prognostic, have been sought to determine the biological behavior of these neoplasms. Immunohistochemistry (IHC) is one of the main tools utilized to biologically differentiate more aggressive tumors from less aggressive ones. However, some immunostainings are influenced by formalin fixation, interfering with the results. This is both a retrospective and prospective study of MCTs diagnosed in laboratory routine. A total of 25 samples, without knowledge about fixation time, were analyzed in the retrospective study, whereas 12 samples, with known fixation times, were assessed in the prospective study. Two histologic grading systems (Patnaik and Kiupel), special staining of toluidine blue, and IHC for KIT and Ki67 proteins were applied in both studies. Additionally, two amplification systems (biotinylated and non-biotinylated) for Ki67 protein and counting of the argyrophilic nucleolar organizing regions (AgNOR method) were tested in the prospective study. In the retrospective study, greater agreement between the evaluating pathologists was observed when the Kiupel system was used. IHC staining for KIT protein was effective in both studies, regardless of fixation time. IHC staining for Ki67 protein was highly sensitive to formaldehyde, and staining failure was observed in 56% of the cases in the retrospective study. In the prospective study, samples fixed for longer than 24 hours showed a reduction in the number of stained cells (altering the determination of the cell growth fraction) or showed absence of IHC staining in both amplification systems. The use of the AgNOR method to evaluate the rate of cell proliferation may be an alternative when the fixation time of the neoplasm is unknown or longer than 24 hours.

show abstract

Section: Discussionmentioning

confidence: 99%

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Alguns autores indicam radioterapia em sarcomas de tecidos moles de alto grau maiores que 5 cm, reduzindo assim as chances de recorrência local (BAPTISTA AM, 2006).O prognóstico para sarcoma sinovial tem relação com índice mitótico, tamanho do tumor, idade, presença de áreas pouco diferenciadas, de áreas rabdóides e de áreas extensas de necrose. Além disso, alto índice de proliferação celular é considerado fator adverso para mortalidade relacionado à doença(LIMA FO, et al, 2005).Diante disso, o presente estudo tem por objetivo relatar um caso incomum de sinoviossarcoma em cavidade bucal, visando seus aspectos histopatológicos, imunohistoquímico e radiográficos. Dessa forma, levar a outros profissionais mais conhecimentos acerca do tema, haja vista que o último relato de cavidade oral foi há mais de 5 anos, e a pesquisa mostra uma atualização sobre essa neoplasia.…”

unclassified

Aspectos histopatológicos e imunohistoquímico do sinoviossarcoma em cavidade bucal

et al. 2019

View full text Add to dashboard Cite

Objetivo: Relatar os aspectos histopatológicos e imunohistoquímicos do sinoviossarcoma em cavidade oral, haja vista que o último relato de cavidade oral foi há mais de 5 anos, este artigo mostra uma atualização sobre essa neoplasia. Detalhamento do caso: Paciente 9 anos de idade do gênero feminino, com sinoviossarcoma localizado em assoalho bucal, assintomático. Foi realizado exames histopatológicos e imunohistoquímicos. Cirurgia de remoção do tumor, e tratamento quimioterápico. Ao estudo imunohistoquímico o caso descrito mostrou positividade para vimentina, citoqueratin Pan e FLI-1. O laudo histopatológico demonstrou se tratar de um sinoviossarcoma do tipo bifásico. Considerações finais: O sinoviossarcoma é uma neoplasia extremamente rara, é um tumor maligno bem definido que representa 5,6% a 10% de todos os sarcomas de tecidos moles, o que, portanto, torna seu diagnóstico mais difícil. O histopatológico e a imunohistoquímica auxiliam de forma precisa no diagnóstico, determinando assim, uma boa abordagem terapêutica. O tratamento consiste, habitualmente, na ressecção cirúrgica oncológica; podendo ser seguida de radioterapia, quimioterapia e mesmo à imunoterapia.

show abstract

Regiões organizadoras nucleolares argirofílicas no sarcoma sinovial

Cited by 2 publications

References 16 publications

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

Aspectos histopatológicos e imunohistoquímico do sinoviossarcoma em cavidade bucal

Contact Info

Product

Resources

About