Immunohistochemistry in diagnostic veterinary pathology: a critical review

Abstract

key words unitermos abstractImmunohistochemistry has become a practical and widely used tool for diagnosis in human pathology since the 70's. However, its application in veterinary diagnostic pathology has not been so common, especially due to the lack of specific antibodies. To overcome this drawback, antibodies which present cross reactivity with human and animal antigens have been applied. The purpose of the present study was to test the cross reactivity of some antibodies intended for the human pathology, which may be used in animal tissues, with the help of antigen retrieval and amplification systems. In the present study it was confirmed that many of the antibodies produced for use in human histopathology might be applied in veterinary pathology. Further studies are needed to increase the list of applicability of these antibodies to different animal species. It must be stressed that in this type of study some variables, such as clone of antibody, dilution, antigen retrieval method, and detection system, have to be evaluated. Lab • v. 41 • n. 4 • p. 263-70 • agosto 2005 a more accurate diagnosis. This fact has brought to the attention of veterinary pathologists the need to improve the application of immunohistochemistry in their daily routine, following the tendency of human diagnostic pathology. Immunohistochemistry

Pathological and immunohistochemical studies on rare cases of primary extragenital transmissible venereal tumours in the mammary gland

“…2 in the present study. However, the detection Ki-67 has proven superior to PCNA in evaluation of the proliferation index in both human and animals tumours (Fournel 1997;Ruiz et al 2005). Nuclear and cytoplasmic staining of PCNA, and nuclear staining of Ki-67 has similarly been reported earlier in cases of TVT in dogs (Ruiz et al 2005).…”

Low CXCL13 Expression, Splenic Lymphoid Tissue Atrophy and Germinal Center Disruption in Severe Canine Visceral Leishmaniasis

“…The immunophenotyping of spleen cells was done using immunohistochemistry as described by Ruiz and collaborators [30] with some modifications. Briefly, serial spleen sections were placed on poly-L-lysine (Sigma-Aldrich, United States)-coated slides and dewaxed.…”

Characterization of Immune Cell Infiltration Into Canine Intracranial Meningiomas

“…The smooth muscle layer of larger blood vessels was consistently stained by the CD79a antibody, as has been previously reported. 53 CD45RA þ cells were found in the majority of tumors (20/23, 87%), although in relatively small numbers. Distribution of these cells was similar to CD3 þ cells, with positive cells found in a perivascular distribution at the tumor-brain interface and smaller numbers interspersed throughout the tumor.…”