Avaliação endócrina e morfológica de transplante ovariano homógeno

Petroianu, Andy; Alberti, Luiz Ronaldo; Vasconcellos, Leonardo de Souza; Leite, Jose O; Castro, Lúcia Porto Fonseca de

doi:10.1590/s1676-24442004000300012

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“…Ovarian tissue contains angiogenic factors that stimulate endothelial cell migration 10,11 , allowing tissue revascularization when heterologous or autologous ovarian grafting is performed [12][13][14] .…”

Section: Discussionmentioning

confidence: 99%

Ovarian blood vessel occlusion as a surgical sterilization method in rats

et al. 2014

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PURPOSE:To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model. METHODS:Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion of the ovarian blood supply using titanium clips, euthanized at 60 or 90 days; and IV (n=20), bilateral occlusion of the ovarian blood supply using nylon thread, euthanized at 60 or 90 days. The estrous cycle was monitored by vaginal cytology. After euthanasia, the reproductive tissues were evaluated histologically. RESULTS:Ovarian atresia was identified macroscopically at 60 days after surgery in the rats in groups III and IV; however, most of the rats in group III maintained cyclicity. Histology of the tissues from group IV revealed that the ovarian tissue was replaced by dense fibrous connective tissue that was slightly vascularized and that intact follicles were absent by 90 days. CONCLUSION:Ovarian blood vessels occluded caused ischemia, leading to progressive tissue necrosis, and bilateral occlusion using a nylon ligature is a viable method for surgical sterilization.

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