Controle de qualidade interlaboratorial em imuno-histoquímica: citoceratinas e receptor de estrógeno como modelos

Alves, Venâncio Avancini Ferreira; Leandro, Luciana de Oliveira; Vassallo, José; Pereira, Emílio Marcelo; Kanamura, Cristina Takami; Wakamatsu, Alda; Santos, Raimunda Telma de Macedo; Nonogaki, Suely

doi:10.1590/s1676-24442004000300008

Cited by 5 publications

(18 citation statements)

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“…Assim, na avaliação de fatores preditivos em câncer de mama, o controle de qualidade das fases pré-analítica, analítica e pós-analítica com atualização constante sobre novos reagentes e critérios de interpretação das reações são necessários, com repercussões importantes na decisão terapêutica dos pacientes (2,13,24,30,39,41) . Na maioria dos casos de câncer de mama do nosso estudo, a avaliação IHQ foi realizada no tumor primário (85% dos casos).…”

Section: Discussionunclassified

“…A experiência do patologista com o método é fator relevante, pois ele interfere na escolha dos reagentes e anticorpos a serem usados em cada caso, analisa a qualidade das reações e interpreta os resultados da coloração. Além disso, o patologista integra os achados morfológicos com os resultados do estudo imuno-histoquímico, formulando o diagnóstico anatomopatológico final (2,13,30) .…”

Section: Introductionunclassified

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Contribuição da imuno-histoquímica na avaliação de fatores prognósticos e preditivos do câncer de mama e no diagnóstico de lesões mamárias

Salles¹,

Cúrcio

Perez³

et al. 2009

J. Bras. Patol. Med. Lab.

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Objetivo: Fazer análise crítica da contribuição da imuno-histoquímica na avaliação de fatores preditivos/ prognósticos do câncer de mama, na pesquisa de micrometástases em linfonodos sentinela e no diagnóstico diferencial de lesões mamárias. Métodos: Foi realizado estudo observacional retrospectivo de todos os casos de lesões mamárias e linfonodos sentinelas submetidas a estudo imuno-histoquímico J Bras Patol Med Lab • v. 45 • n. 3 • p. 213-222 • junho 2009

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Section: Discussionunclassified

Section: Introductionunclassified

Contribuição da imuno-histoquímica na avaliação de fatores prognósticos e preditivos do câncer de mama e no diagnóstico de lesões mamárias

Salles¹,

Cúrcio

Perez³

et al. 2009

J. Bras. Patol. Med. Lab.

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“…It is an important tool for scientific research and also a complementary technique in the elucidation of differential diagnosis which are not determinable by conventional analysis with hematoxylin and eosin 2,22–24,26,28–37. The great improvement in the contribution and application of immunohistochemistry in pathological anatomy became known as the “brown revolution” of the histopathology laboratory 22…”

Section: The Immunohistochemistry Techniquementioning

confidence: 99%

“…2); 2) subtyping of neoplasias (such as lymphomas, for example); 3) characterization of primary site of malignant neoplasias; 4) research for prognostic factors and therapeutic indications of some diseases; 5) discrimination of benign versus the malign nature of certain cell proliferations (Fig. 3); identification of structures, organisms and materials secreted by cells 2,22,23,26,36,38…”

Section: The Immunohistochemistry Techniquementioning

confidence: 99%

“…The usefulness and contribution of immunohistochemistry in solving problems in pathological anatomy is directly proportionate to the experience of the hands that perform the reactions and also the eyes that interpret the results 2,22,24,36,38. Therefore, even though very simple in concept, immunostaining methods requires rigor of execution and may present significant bias.…”

Section: The Immunohistochemistry Techniquementioning

confidence: 99%

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Immunohistochemistry as an Important Tool in Biomarkers Detection and Clinical Practice

et al. 2010

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The immunohistochemistry technique is used in the search for cell or tissue antigens that range from amino acids and proteins to infectious agents and specific cellular populations. The technique comprises two phases: (1) slides preparation and stages involved for the reaction; (2) interpretation and quantification of the obtained expression. Immunohistochemistry is an important tool for scientific research and also a complementary technique for the elucidation of differential diagnoses which are not determinable by conventional analysis with hematoxylin and eosin. In the last couple of decades there has been an exponential increase in publications on immunohistochemistry and immunocytochemistry techniques. This review covers the immunohistochemistry technique; its history, applications, importance, limitations, difficulties, problems and some aspects related to results interpretation and quantification. Future developments on the immunohistochemistry technique and its expression quantification should not be disseminated in two languages—that of the pathologist and another of clinician or surgeon. The scientific, diagnostic and prognostic applications of this methodology must be explored in a bid to benefit of patient. In order to achieve this goal a collaboration and pooling of knowledge from both of these valuable medical areas is vital

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Immunohistochemical Expression of Cyclooxygenases in Hypertrophic Scars and Keloids

Pavelecini

Zettler

Fernandes

et al. 2019

Plastic and Reconstructive Surgery - Global Open

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Background:There are studies demonstrating an increased expression of cyclooxygenase (COX) in keloids and hypertrophic scars, suggesting that anti-inflammatory drugs could be used in their treatment. However, a precise relationship between COX and pathological scarring has not been established in the literature yet. This study aims to evaluate the immunohistochemical expression of COXs in these scars.Methods:Prospective study, including 54 patients (aged 18–60 years) undergoing scar excision: 18 normal scars (group 1), 18 hypertrophic scar (group 2), and 18 keloids (group 3). The group classification was performed by clinical criteria. Scars samples were collected and anatomopathological examination (through hematoxylin-eosin method) was performed to confirm the scar type. Immunohistochemistry was performed to assess the expression of COX1 and COX2 in epidermis and dermis. Results were compared among all groups and between group I versus II and III together (abnormal scars).Results:For COX1, in the epidermis, there was no significant difference in the immunohistochemical expression when comparing the 3 groups. In the dermis, groups 2 and 3 had greater expression than group 1, with a significant difference being found when comparing all groups (P = 0.014), and in the comparison between normal versus abnormal scars (P = 0.004). For COX2, there was no significant difference between the groups in both the epidermis and dermis.Conclusions:The immunohistochemical expression of COX1 was greater in the dermis of abnormal scars when compared with normal scars. Future studies can be performed involving COX blockade as a perspective of these scars treatment.

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Controle de qualidade interlaboratorial em imuno-histoquímica: citoceratinas e receptor de estrógeno como modelos

Cited by 5 publications

References 0 publications

Contribuição da imuno-histoquímica na avaliação de fatores prognósticos e preditivos do câncer de mama e no diagnóstico de lesões mamárias

Contribuição da imuno-histoquímica na avaliação de fatores prognósticos e preditivos do câncer de mama e no diagnóstico de lesões mamárias

Immunohistochemistry as an Important Tool in Biomarkers Detection and Clinical Practice

Immunohistochemical Expression of Cyclooxygenases in Hypertrophic Scars and Keloids

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