Elevada diversidade genética interpopulacional em Oryza glumaepatula Steud. (Poaceae) avaliada com microssatélites

Silva, Cynthia Maria; Karasawa, Marines Marli Gniech; Vencovsky, Roland; Veasey, Elizabeth Ann

doi:10.1590/s1676-06032007000200019

Cited by 5 publications

(6 citation statements)

References 24 publications

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“…A lower F ST value (0.491) was obtained by Karasawa et al (2007) with microsatellite markers for O. glumaepatula populations, although similar values were obtained by Brondani et al (2005) (F ST = 0.847) and Silva et al (2007) (F ST = 0.715). The fact that most of the isozymic variability was distributed between rather than within species is in accordance with the predominantly autogamic mating system of these species, especially the diploid O. glumaepatula (Akimoto et al, 1998;Buso et al, 1998;Brondani et al, 2005;Karasawa et al, 2007a,b;Vaz et al, 2009).…”

Section: Resultssupporting

confidence: 54%

Genetic diversity of American wild rice species

Veasey¹,

Bressan²,

Zucchi³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Studies on genetic diversity and genetic structure of natural populations are important in order to define strategies for in situ and ex situ conservation actions and for plant pre-breeding programs. Aiming to assess the genetic diversity and genetic structure of three wild American Oryza species with isozyme markers, 14 populations of the diploid O. glumaepatula (A gl A gl ), 11 populations of the tetraploid O. grandiglumis (CCDD) and five populations of the also tetraploid O. latifolia (CCDD) were studied. They were all originated from Rio Paraguay hydrographic basin and the Amazon. Four enzymes were used and they gave 40 polymorphic bands. The most polymorphic species was O. glumaepatula, followed by O. latifolia and O. grandiglumis. A cluster analysis with the Jaccard similarity coefficient separated the diploid from the two tetraploid species, and also the two tetraploid species. This separation was also evident on a scatter plot from a principal component analysis, suggesting that they should be treated as two separate species, although further studies are necessary to provide support for this affirmative. The AMOVA analyses showed a high intrapopulational variability for O. latifolia (67.6%) and O. grandiglumis (52.2%), when compared to their interpopulational variability (32.4% and 47.8%, respectively), which suggests the hypothesis of a higher degree of outcrossing events within these species. When studying the correlation between the Jaccard dissimilarity coefficient and geographic distances, a spatial genetic structure was observed for O. glumaepatula only. These results are important for defining strategies of both in situ and ex situ conservation.

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Section: Resultssupporting

confidence: 54%

Genetic diversity of American wild rice species

Veasey¹,

Bressan²,

Zucchi³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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“…However, the authors observed greater intrapopulational than interpopulational variability, a pattern usually found in the crossfertilized populations, which was explained considering the life history characteristics of this species. Studies of the genetic structure of O. glumaepatula populations were also recently conducted with microsatellite markers (Karasawa, 2005;Karasawa et al, 2007;Brondani et al, 2005;Silva et al, 2007), showing high values for F ST and R ST , suggesting a higher interpopulational variability, as well as the predominance of a selffertilizing breeding system. The purpose of this study, using isozymic markers, was to provide further information on the genetic diversity and genetic structure of 14 O. glumaepatula populations, originated from the hydrographic basins of the Amazon and of Rio Paraguay in the Pantanal ecosystem.…”

Section: Introductionmentioning

confidence: 99%

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Veasey

Cardin

Silva

et al. 2008

Braz. arch. biol. technol.

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“…Microsatellites are considered genetically more informative than other markers, especially when dealing with populations with low genetic diversity content at the DNA level (Paetkau et al 1995). The low gene diversity (H e ) levels in this species has already been shown in previous studies with isozymes, revealing variations from 0.044 to 0.060 (Akimoto et al 1998, Buso et al 1998, Veasey et al 2008, as compared with microsatellites with higher levels, ranging from 0.113 to 0.491 (Brondani et al 2005, Karasawa et al 2007a, Silva et al 2007. Thus, considering the information obtained in this study with isozymes and microsatellites, we can conclude that microsatellite markers are in fact more informative as they were able to detect higher levels of intra-population diversity in this species.…”

Section: Genetic Diversity Levelsmentioning

confidence: 70%

“…Previous studies on genetic structure conducted in the genus Oryza have recorded several F ST (G ST ) values, but largely agree that the mode of reproduction of the species has generated prominent effect on the differentiation observed. In O. glumaepatula, studies using allozymes showed F ST values of 0.346 (Akimoto et al 1998), 0.310 (Buso et al 1998) and 0.763 (Veasey et al 2008), while those using microsatellites showed values such as 0.847 (Brondani et al 2005), 0.491 (Karasawa et al 2007a and 0.715 (Silva et al 2007). In progeny studies, the value recorded with microsatellites was 0.451 for the parental populations, and 0.284 for the families of these populations (Karasawa et al 2007b).…”

Section: Genetic Diversity Levelsmentioning

confidence: 96%

“…The advantages of this marker are the codominant nature, high frequency and random distribution in genomes and the high polymorphism it usually shows (Schlötterer 2004). Microsatellites have been used in several conservation and genetic diversity studies in wild rice species (Gao et al 2002b, Zhou et al 2003, Gao 2005, Gao et al 2006, Xu et al 2006, Wang et al 2008, and specifically in studies of Oryza glumaepatula natural populations, including the genetic structure and diversity (Brondani et al 2005, Karasawa et al 2007a, Silva et al 2007, Vaz et al 2009), the mating system determination (Karasawa et al 2007b, Vaz et al 2009, genetic mapping (Brondani et al 2001) and phylogeny (Bautista et al 2001).…”

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confidence: 99%

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Comparison of microsatellites and isozymes in genetic diversity studies of Oryza glumaepatula (Poaceae) populations

Karasawa

Vencovsky

Silva

et al. 2012

RBT

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The study of the genetic structure of wild plant populations is essential for their management and conservation. Several DNA markers have been used in such studies, as well as isozyme markers. In order to provide a better comprehension of the results obtained and a comparison between markers which will help choose tools for future studies in natural populations of Oryza glumaepatula, a predominantly autogamous species, this study used both isozymes and microsatellites to assess the genetic diversity and genetic structure of 13 populations, pointing to similarities and divergences of each marker, and evaluating the relative importance of the results for studies of population genetics and conservation. A bulk sample for each population was obtained, by sampling two to three seeds of each plant, up to a set of 50 seeds. Amplified products of eight SSR loci were electrophoresed on non-denaturing polyacrylamide gels, and the fragments were visualized using silver staining procedure. Isozyme analyses were conducted in polyacrylamide gels, under a discontinuous system, using six enzymatic loci. SSR loci showed higher mean levels of genetic diversity (A=2.83, p=0.71, A P =3.17, H o =0.081, H e =0.351) than isozyme loci (A=1.20, p=0.20, A P =1.38, H o =0.006, H e =0.056). Interpopulation genetic differentiation detected by SSR loci (R ST =0.631, equivalent to F ST =0.533) was lower than that obtained with isozymes (F ST =0.772). However, both markers showed high deviation from Hardy-Weinberg expectations (F IS =0.744 and 0.899, respectively for SSR and isozymes). The mean apparent outcrossing rate for SSR (=0.14) was higher than that obtained using isozymes (=0.043), although both markers detected lower levels of outcrossing in Amazonia compared to the Pantanal. The migrant number estimation was also higher for SSR (Nm=0.219) than isozymes (Nm=0.074), although a small number for both markers was expected due to the mode of reproduction of this species, defined as mixed with predominance of self fertilization. No correlation was obtained between genetic and geographic distances with SSR, but a positive correlation was found between genetic and geographic distances with isozymes. We conclude that these markers are divergent in detecting genetic diversity parameters in O. glumaepatula and that microsatellites are powerful for detecting information at the intra-population level, while isozymes are more powerful for inter-population diversity, since clustering of populations agreed with the expectations based on the geographic distribution of the populations using this marker.

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Elevada diversidade genética interpopulacional em Oryza glumaepatula Steud. (Poaceae) avaliada com microssatélites

Cited by 5 publications

References 24 publications

Genetic diversity of American wild rice species

Genetic diversity of American wild rice species

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Comparison of microsatellites and isozymes in genetic diversity studies of Oryza glumaepatula (Poaceae) populations

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