Accuracy of two hand-held electronic devices for determination of blood β-hydroxybutyrate in dairy cows

Fiorentin, Eliana Lucia; Tiecher, Anderson; Menegat, Cristina; Soares, Clarice; Aires, Adelina Rodrigues; Rocha, Ricardo Xavier da; González, Félix Hilario Díaz

doi:10.1590/s1519-99402017000300004

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…The BHB concentration in blood was measured immediately after blood sampling, using a handheld device (FreeStyle Optium Blood Glucose and Ketone Monitoring System, Abbott Diabetes Care Ltd., Maidenhead, Berkshire, UK), as per the manufacturer's instructions. This handheld device has been recently validated previously for use on dairy cows and is known to provide very accurate results (Fiorentin et al, 2017). All blood samples were collected at approximately the same time of the day (0700 h; before the AM milking and before a fresh allocation of pasture and supplementary feed were offered), between June and October 2016.…”

Section: Animals and Reference Analysismentioning

confidence: 99%

Prediction of blood β-hydroxybutyrate content and occurrence of hyperketonemia in early-lactation, pasture-grazed dairy cows using milk infrared spectra

Bonfatti

Turner

Kuhn-Sherlock

et al. 2019

Journal of Dairy Science

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The objective of this study was to evaluate the ability of milk infrared spectra to predict blood β-hydroxybutyrate (BHB) concentration for use as a management tool for cow metabolic health on pasturegrazed dairy farms and for large-scale phenotyping for genetic evaluation purposes. The study involved 542 cows (Holstein-Friesian and Holstein-Friesian × Jersey crossbreds), from 2 farms located in the Waikato and Taranaki regions of New Zealand that operated under a seasonal-calving, pasture-based dairy system. Milk infrared spectra were collected once a week during the first 5 wk of lactation. A blood "prick" sample was taken from the ventral labial vein of each cow 3 times a week for the first 5 wk of lactation. The content of BHB in blood was measured immediately using a handheld device. After outlier elimination, 1,910 spectra records and corresponding BHB measures were used for prediction model development. Partial least square regression and partial least squares discriminant analysis were used to develop prediction models for quantitative determination of blood BHB content and for identifying cows with hyperketonemia (HYK). Both quantitative and discriminant predictions were developed using the phenotypes and infrared spectra from two-thirds of the cows (randomly assigned to the calibration set) and tested using the remaining one-third (validation set). A moderate accuracy was obtained for prediction of blood BHB. The coefficient of determination (R 2 ) of the prediction model in calibration was 0.56, with a root mean squared error of prediction of 0.28 mmol/L and a ratio of performance to deviation, calculated as the ratio of the standard deviation of the partial least squares model calibration set to the standard error of predic-tion, of 1.50. In the validation set, the R 2 was 0.50, with root mean squared error of prediction values of 0.32 mmol/L, which resulted in a ratio of performance to deviation of 1.39. When the reference test for HYK was defined as blood concentration of BHB ≥1.2 mmol/L, discriminant models indicated that milk infrared spectra correctly classified 76% of the HYK-positive cows and 82% of the HYK-negative cows. The quantitative models were not able to provide accurate estimates, but they could differentiate between high and low BHB concentrations. Furthermore, the discriminant models allowed the classification of cows with reasonable accuracy. This study indicates that the prediction of blood BHB content or occurrence of HYK from milk spectra is possible with moderate accuracy in pasture-grazed cows and could be used during routine milk testing. Applicability of infrared spectroscopy is not likely suited for obtaining accurate BHB measurements at an individual cow level, but discriminant models might be used in the future as herd-level management tools for classification of cows that are at risk of HYK, whereas quantitative models might provide large-scale phenotypes to be used as an indicator trait for breeding cows with improved metabolic health.

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Section: Animals and Reference Analysismentioning

confidence: 99%

Prediction of blood β-hydroxybutyrate content and occurrence of hyperketonemia in early-lactation, pasture-grazed dairy cows using milk infrared spectra

Bonfatti

Turner

Kuhn-Sherlock

et al. 2019

Journal of Dairy Science

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“…Neste estudo, no período pré-parto nenhum animal foi verificado com níveis superiores a 1,2 mmol/L, já no período pós parto 8 animais do sistema à pasto e 2 do sistema confinado apresentaram índices superiores compatíveis com cetose subclínica, ou seja, 47% dos animais do sistema à pasto e apenas 11% do sistema confinado. Estudos realizados por Fiorentin et al (2017) demonstraram que o dosímetro portátil Optium XCeed ® foi capaz de fornecer resultados rápidos e de boa eficácia na detecção dos teores de Betahidroxibutirato no sangue e após, Souza & Souza (2015) comprovaram que o dosímetro ketovet ® desenvolvido especificamente para bovinos foi capaz de fornecer resultados rápidos e de boa eficácia na detecção dos teores de Betahidroxibutirato no sangue. Este estudo ao comparar ambos os dosímetros portáteis pré e pós-parto não houve diferença significativa, mostrando que ambos os dosímetros são eficientes na detecção da cetose bovina (Tabela 6), independente do período avaliado.…”

Section: Resultsunclassified

Cetosis en vacas de leche, en diferentes sistemas de producción, por Optium Xceed® y Ketovet®

Schneider¹,

Fraga²,

Martins³

et al. 2020

Pubvet

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O estudo teve como objetivo comparar dois aparelhos portáteis (Optium Xceed® e Ketovet®) para o diagnóstico de cetose subclínica no campo; conhecer a prevalência desta patologia na região noroeste do estado do Rio Grande do Sul, em sistemas à pasto e confinado; avaliar a composição do leite de vacas em diferentes níveis de corpos cetônicos e glicose no sangue. Os dados foram coletados de três propriedades leiteiras do município de Augusto Pestana, Rio Grande do Sul, Brasil, sendo duas propriedades em sistema de manejo alimentar à pasto e uma em sistema de Compost Barn, sendo todos os bovinos da raça holandesa. Com a realização deste estudo conclui-se que a cetose apresenta prevalência maior, em sistema à pasto no período pós-parto devido à alta exigência de energia para a produção de leite dos animais; ambos os aparelhos Ketovet® e Optium® são eficazes no diagnóstico de cetose subclínica independente do período (pré ou pós-parto) e dos sistemas avaliados (à pasto ou confinado) e a ocorrência de cetose subclínica não causa alterações significativas na composição do leite de vacas. A cetose é um tema de extrema importância na bovinocultura de leite, sendo um assunto encontrado diariamente na rotina veterinária, revelando que é possível realizar o diagnóstico de forma rápida e com baixo custo à campo, possibilitando evitar perdas sanitárias e financeiras aos rebanhos.

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“…Glucose (catalog n. 113; wave-length 505 nm), triglycerides (catalog n. 87; wave-length 505 nm), and total cholesterol (catalog n. 76; wave-length 500 nm) were assayed colorimetrically (Kasuaki IL-120 UV-Vis Spectrophotometer) using Labtest kits ( Labtest, 2022 ). β-hydroxybutyrate was quantified using a FreeStyle Optium (FSO, Abbott) portable device, following validation for non-human vertebrates ( Fiorentin et al, 2017 ; Ribeiro et al, 2021 ). All assays were carried out strictly following the manufacturer’s instructions and with previously sterilized material to avoid contamination.…”

Section: Methodsmentioning

confidence: 99%

Unraveling Metabolite Provisioning to Offspring Through Parental Fluids: A Case Study of the Brazilian Guitarfish, Pseudobatos horkelii

Wosnick

Leite²,

Giareta³

et al. 2022

Front. Physiol.

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Elasmobranchs have a very distinct metabolism, and many aspects related to the energetic dynamics of these animals remain poorly investigated. The reproductive period is particularly vulnerable for viviparous species, as part of the energy reserves of the parental biomass is reallocated for gamete production and embryo development. In this context, this study aimed to characterize parental metabolite provisioning to the offspring (both sperm and developing embryos) of the Brazilian Guitarfish, Pseudobatos horkelii, through glucose, β-hydroxybutyrate, triglycerides, and total cholesterol determinations in the uterine liquid (UL) and serum of pregnant females and in the seminal fluid (SF) and serum of males during the copulation period. No significant difference was observed for the analyzed markers between the UL and SF. Except for triglycerides, higher in female serum samples, all other energy markers were present at similar concentrations in the serum of both females and males. When comparing female UL and serum, significant differences were observed for triglycerides and total cholesterol. No differences were observed between SF and serum in males. The results indicate that all markers are being made available to offspring, possibly complementary to the yolk in the case of maternal liquid, and as an additional source for sperm mobilization required during egg fertilization in the case of the paternal fluid. Correlations between the markers in the parental matrices were also noted, compatible with the metabolic pathways activated during energy mobilization in vertebrates. Moreover, distinct marker predominance patterns were also noted for both UL and SF. Energy mobilization characterization directed to offspring through parental fluids aids in unraveling metabolic dynamics during the reproduction stage while also providing support for stress physiology studies to evaluate the indirect effects of parental allostatic overload in both sperm and developing embryos. Finally, energy mobilization assessments of parental fluids may also help elucidate how internal fertilization and viviparity evolved in this very distinct taxonomic group.

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Accuracy of two hand-held electronic devices for determination of blood β-hydroxybutyrate in dairy cows

Cited by 9 publications

References 10 publications

Prediction of blood β-hydroxybutyrate content and occurrence of hyperketonemia in early-lactation, pasture-grazed dairy cows using milk infrared spectra

Prediction of blood β-hydroxybutyrate content and occurrence of hyperketonemia in early-lactation, pasture-grazed dairy cows using milk infrared spectra

Cetosis en vacas de leche, en diferentes sistemas de producción, por Optium Xceed® y Ketovet®

Unraveling Metabolite Provisioning to Offspring Through Parental Fluids: A Case Study of the Brazilian Guitarfish, Pseudobatos horkelii

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