A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

Praxedes, P G; Zerlin, Juliana Kuroiva; Dias, Lourdes; Pessoni, Rosemeire Aparecida Bom

doi:10.1590/s1519-69842011000400013

Cited by 7 publications

(4 citation statements)

References 24 publications

(28 reference statements)

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“…No information is available on the antimicrobial activity of medium/high polarity secondary metabolites of S. virgata, P. molluginifolia, and C. mutabilis, which proved effective for inhibiting yeast growth in the disk diffusion test in the present study. S. virgata in-hibits filamentous fungi through the mediation of FP1-A protein [31].…”

Section: Discussionmentioning

confidence: 99%

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Brito

Cassemiro

Souza

et al. 2021

Planta Medica International Open

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The Pantanal wetland harbors a rich flora with uncharted pharmacological potential. This study evaluated 20 Brazilian Pantanal plants against Candida albicans, C. parapsilosis, C. tropicalis, and C. krusei. Fungal susceptibility was determined by agar diffusion and broth microdilution; active compounds were identified by bioautography and HPLC-DAD-MS/MS. Sesbania virgata, Polygala molluginifolia, and Cantinoa mutabilis extracts and their chloroform and ethyl acetate (EtOAc) fractions exhibited the best activity against all Candida species tested. The EtOAc fraction of P. molluginifolia proved to be more efficient in inhibiting C. parapsilosis and C. krusei growth (Minimum inhibitory concentration of 125 and 62.5 μg/mL, respectively). Bioautography of this fraction revealed two active bands, characterized by HPLC-DAD-MS/MS as a mixture of podophyllotoxin derivatives blumenol, besides some flavonoids. This work demonstrated antifungal potential of P. molluginifolia podophyllotoxin derivatives and the versatility of bioautography with HPLC-DAD-MS/MS to identify the bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Brito

Cassemiro

Souza

et al. 2021

Planta Medica International Open

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“…In turn, the MIC required to promote inhibition of A. flavus and P. citrinum is criteria. Another record of antifungal activity was found for protein from S. virgata seeds (PRAXEDES et al, 2011). This antifungal protein is toxic to Aspergillus niger, Cladosporium cladospoirides and Colletotrichum gloesporioides, reinforcing that S. virgata seeds are excellent sources of antifungal biomolecules.…”

Section: Antifungal Activitymentioning

confidence: 94%

Nutritional composition and biological activities (antioxidant and antifungal) of Sesbania virgata (Cav.) Pers. seeds

Sá

Silva

et al. 2021

R. bras. Tecnol. Agroindustr.

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Sesbania virgata (Cav.) Pers. is a Pantropical legume (Fabaceae) able to colonize riverbanks in Brazilian semiarid regions, commonly used to recover degraded soils. Although its seeds have high nutritional value, are little explored for biotechnological and biological applications. In order to begin to fill the void existing in this theme, the present research described the nutritional composition of the S. virgata seeds, in addition to its antioxidant and antimicrobial activities. The energy value of S. virgata seeds was 366.6 kcal 100g-1, and among the investigated macronutrients, the protein content stands out (60.8%). However, the carbohydrate and crude fat contents are also promising, highlighting the abundance in polyunsaturated fatty acids, especially linoleic acid and linolenic acid. S. virgata seeds are an excellent source of essential (leucine, lysine and valine) and non-essential amino acids (glutamic acid, aspartic acid and arginine). Under the assay conditions, lectins and trypsin inhibitors were not demarcated. Additionally, S. virgata seeds confer antifungal activity against Candida albicans, Candida tropicalis, Aspergillus flavus and Penicillium citrinum, and antioxidant activity, for ABTS•+ and DPPH• scavenging methods. In view of these findings, the nutritional composition of S. virgata seeds encourages its use as natural source of functional products, and its biological activities stimulate its biotechnological and pharmacological application as exogenous antifungal and antioxidant agent.

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“…Previous studies (Ghosh 2009), including other Sesbania species (Ajitha et al 2016, Maregesi et al 2008, report the antifungal activity of proteins with lectin activity obtained from plants, with molecular masses similar to PFLA, showing antifungal activity against the pathogens investigated in this research. Praxedes et al (2011) report the occurrence and concentration of antifungal proteins in a fraction with molecular mass similar to PFLA, obtained from S. virgata seeds. Other antifungal protein fractions are reported whose antifungal activity was attributed to its lectins (Silva et al 2019, Gautam et al 2018, Gupta et al 2018.…”

Section: An Acad Brasmentioning

confidence: 99%

Protein fraction from Sesbania virgata (Cav.) Pers. seeds exhibit antioxidant and antifungal activities

SÁ,

GADELHA,

FRAGOSO

et al. 2024

An. Acad. Bras. Ciênc.

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Sesbania virgata (Cav.) Pers. seeds are protein sources with health and environmental benefits. In this research, proteins with lectin activity were identified in a protein fraction from S. virgata seeds (PFLA), as well its antioxidant and antimicrobial potentials, in addition to cytotoxic effects. To obtain PFLA, seed flour was homogenized in Glycine-NaOH (100 mM; pH 9.0; NaCl 150 mM) and precipitated in ammonium sulfate.PFLA concentrates bioactive lectins (32 HU/mL, 480 HU/gFa, 18.862 HU/mgP) and essential amino acids (13.36 g/100g protein). PFLA exerts antioxidant activity, acting as a promising metal chelating agent (~77% of activity). Analyzes of cell culture assay results suggest that antioxidant activity of PFLA may be associated with the recruitment of essential molecules to prevent the metabolic impairment of cells exposed to oxidative stress. PFLA (256 -512 µg/mL) also exhibits antifungal activity, inhibiting the growth of Aspergillus flavus, Candida albicans, Candida tropicalis and Penicillium citrinum. Cytotoxic analysis indicates a tendency of low interference in the proliferation of 3T3 andHepG2 cells in the range of PFLA concentrations with biological activity. These findings support the notion that PFLA is a promising adjuvant to be applied in current policies on the management of metal ion chelation and fungal infections.

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A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

Cited by 7 publications

References 24 publications

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Nutritional composition and biological activities (antioxidant and antifungal) of Sesbania virgata (Cav.) Pers. seeds

Protein fraction from Sesbania virgata (Cav.) Pers. seeds exhibit antioxidant and antifungal activities

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