Apoptotic ratios and mitotic abnormalities in 17-β-estradiol-transformed human breast epithelial MCF-10F cells

Abstract

Treatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in molecular biology and image analysis assays, no cytological data on apoptotic ratios and mitotic abnormalities have been established to accompany the various steps leading to 17-β-estradiol-treated MCF-10F cells to tumorigenesis. Here we detected that the apoptotic ratio decreases with the transformation and tumorigenesis progress, except for the tumor cell line C5-A8-T8, probably on account of its more intense proliferation rate and a more rapid culture medium consumption. Increased frequency of mitotic abnormalities contributed by triple- and tetrapolar metaphases, and by lagging chromosomes and chromosome bridges observed at the anaphase found by transformation and tumorigenesis progress. However, no difference was found under these terms when the C5-A6-T6 and C5-A8-T8 tumor cell lines were compared to each other. Present findings are in agreement with the nuclear instability and enrichment of dysregulated genes in the apoptotic process promoted by transformation and tumorigenesis in 17-β-estradiol-treated MCF-10F cells.

Molecular characterization and pathology of field isolates of foot-and-mouth virus in Swiss albino mice

“…The lesions scoring was analyzed statistically by Nonparametric, Mann-Whitney test and the results were found significant with P-value<0.05 (Cruz et al, 2011). According to Arzt et al (2011), disease pathogenesis in adult mice depends on the mouse strain and FMDV strain just like the natural host.…”

Orthotopic tumorgrafts in nude mice as a model to evaluate calcitriol effects in breast cancer

“…This preclinical model enables direct analysis of patient derived tumor samples (Zhang et al, 2013), as well as concomitant evaluation of hormone adverse effects. Although immunocompromised mice have been used to evaluate hormone actions in breast cancer xenografts, established from cell lineages (Cruz et al, 2001), this is the first effort to evaluate calcitriol effects in tumorgrafts.…”