2010
DOI: 10.1590/s1519-69842010005000008
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Alternative method for quantification of alfa-amylase activity

Abstract: A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL -1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of enzyme activities… Show more

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Cited by 12 publications
(5 citation statements)
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“…Several authors have published pedagogical articles describing experiments on α-amylase activity. ,, Most of them are starch–-iodine methods, which underestimate the α-amylase inhibitory potential of some compounds and extracts. , Moreover, the published studies evaluate α-amylase from saliva, which physiologically accounts for 10–30% of starch cleavage to shorter oligomers . On the other hand, α-amylase from the pancreas plays an important role in postprandial hyperglycemia. , As mentioned above, we used porcine pancreatic α-amylase in the present study, which has a high level of similarity when compared to the human pancreatic enzyme.…”
Section: Introductionmentioning
confidence: 99%
“…Several authors have published pedagogical articles describing experiments on α-amylase activity. ,, Most of them are starch–-iodine methods, which underestimate the α-amylase inhibitory potential of some compounds and extracts. , Moreover, the published studies evaluate α-amylase from saliva, which physiologically accounts for 10–30% of starch cleavage to shorter oligomers . On the other hand, α-amylase from the pancreas plays an important role in postprandial hyperglycemia. , As mentioned above, we used porcine pancreatic α-amylase in the present study, which has a high level of similarity when compared to the human pancreatic enzyme.…”
Section: Introductionmentioning
confidence: 99%
“…Detection and Activity of Enzymatic Proteins. Cell wall degrading enzyme activities in mycelia and sclerotial exudates were visually screened by published protocols, including GLU, 32 MAN, 33 AMY, 34 and PG. 35 Briefly, a mycelial plug or 20 μL of sclerotial exudates was incubated on detection media (i.e., water agar medium with the corresponding substrates) and the plates were investigated at 24 h after inoculation (hai).…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…The enzyme glucose oxidase (GOD) is used to determine blood glucose level, total starch content in cereal products, and serum α ‐amylase activity because it is cheap, stable, and is highly specific for glucose. Since GOD is highly specific for glucose, the product of α ‐amylase (maltose and other dextrins; Eqn (5)) cannot be used directly with GOD to determine α ‐amylase activity and, therefore, is concomitantly hydrolysed by an amyloglucosidase to yield glucose (Eqn (6)): StarchαAmylaseMaltose+Maltotriose+Dextrins Maltose+DextrinsAmyloglucosidaseGlucose …”
Section: Methods Used To Assess α‐Amylase Activitymentioning
confidence: 99%