Evaluation of a new formulation of Bacillus thuringiensis israelensis

Lopes, José; Arantes, O. M. N.; Cenci, M. A.

doi:10.1590/s1519-69842010000500029

Cited by 9 publications

(5 citation statements)

References 12 publications

(10 reference statements)

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“…Comparable to results cited by (Zaki, et al, 2020& Lopez et al, 2010, and Scott et al, 2010, who stated, in similar results of this study, that, Bacillus thuringiensis is an effective biological insecticide for killing mosquito larvae. However, choosing the suitable application method for larviciding is critical in increasing its effectiveness.…”

Section: Discussionsupporting

confidence: 84%

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Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Abeer

2022

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Flies and mosquitoes are public health threats by transmitting various human diseases. Culex and Aedes species are insect vectors of several diseases such as malaria and dengue fever (DF). DF is a human arbovirus disease transmitted by the female mosquito of the genus Aedes, mainly Aedes aegypti, which is the most serious mosquito-borne viral disease worldwide and endemic in certain cities of Saudi Arabia, such as Jazan area, (Alhaeli et al., 2016). And because of the climate of this area enhances the breeding of mosquitoes, especially during rainfall and high humidity. Another factor is the water storage in containers that served as natural habitats for mosquitoes, (Elisa et al., 2014). Chemical-insecticides used in pest control programs, are toxic for both humans and the environment, also, many insect species gained resistance to the insecticides. Therefore, there is a need to use effective and safe alternative methods to eliminate pests. According to (Boisvert, 2007), Bacillus thuringiensis subsp israelensis was isolated for the first time in 1976 and found to be toxic to mosquito larvae. B.t.t. has entomopathogenic effects because of the crystal proteins produced during the sporulation stage. The crystal proteins paralyze the microvilli of the insect's digestive tract which leads to death by septicemia (Angelo et al., 2010). The application of bio-control agents against insects that carry human pathogens is considered

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Section: Discussionsupporting

confidence: 84%

“…The end product of this formulation was a fine-sized gray powder that dispersed easily when mixed with water, containing 3 x 10 7 CFU/gm. (Lopez et al, 2010).…”

Section: Preparation Of ƃ-Toxin Formulationmentioning

confidence: 99%

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Abeer

2022

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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“…Biopesticides based on Bacillus thuringiensis have become a reliable tool in agricultural pest control, forestry and health-related conditions (Lopes et al, 2010). This gram-positive bacterium has been isolated worldwide and characterised by different techniques, including PCR screening to detect the cry genes.…”

Section: Introductionmentioning

confidence: 99%

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Fiúza¹,

Schünemann

Pinto

et al. 2012

Braz. J. Biol.

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Bacillus thuringiensis is a bacterium used for biopesticides production and pest-resistant plants due to the synthesis of protein crystals by cry genes, which are effective in controlling several insect orders such as Lepidoptera. This work aimed at the evaluation and characterisation of two new B. thuringiensis isolates active against A. gemmatalis (Hübner 1818) larvae, which is the soybean major pest. The results showed that Bt117-4 isolate amplified fragments corresponding to cry2 and cry9 genes, and synthesised protein fragments equivalent to 130, 90 and 45 kDa. The Bt3146-4 isolate amplified DNA fragments corresponding to cry9 gene and synthesised protein fragments of 70, 58 and 38 kDa. Transmission electron microscopy revealed the presence of protein crystals in both isolates. CL50 with Cry purified proteins from Bt117-4 and Bt3146-4, corresponded to 0.195 and 0.191 µg larvae-1, respectively. The two B. thuringiensis isolates selected in this study were effective to control velvetbean caterpillar at laboratory conditions. Field tests should be carried on to develop new biopesticides formulation as well for cry genes resource for Anticarsia gemmatalis resistant transgenic plants.

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“…quinquefasciatus larvae and also presented high efficiency compared to most of the tested commercial products. The bio-insecticide stability is 90 days, approximately, therefore, its production is viable (Lopes et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Strain of Bacillus thuringiensis from Restinga, toxic to Aedes (Stegomyia) aegypti (Linnaeus) (Diptera, Culicidae)

et al. 2021

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Bacillus thuringiensis is the most commonly used entomopathogen in the control of Aedes aegypti, which is a vector for different etiological agents that cause serious infections in humans. Several studies aim to isolate strains of this bacterium from different environments, with the perspective of selecting isolates with larvicidal activity for mosquitoes. Aiming at the insecticidal action of B. thuringiensis, the present study aimed to prospect B. thuringiensis of restinga and mangrove soils from the state of Maranhão, Brazil, with toxic potential for use in the biological control of Ae. aegypti. Bioassays were performed to determine the entomopathogenic activity of the bacilli against Ae. aegypti and lethal concentrations (LC50 and CL90) were estimated after the tests. Polymerase Chain Reaction and SDS-PAGE techniques were performed to verify the gene and protein content of the isolates, respectively. The soil of the mangrove and restinga ecosystems showed potential for obtaining B. thuringiensis. This isolate, in addition to having proteins with molecular mass similar to the toxins Cry and Cyt, also presented several diptera-specific genes cry and cyt, demonstrating that it has high potential to be used in the biological control of Ae. aegypti.

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Evaluation of a new formulation of Bacillus thuringiensis israelensis

Cited by 9 publications

References 12 publications

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Strain of Bacillus thuringiensis from Restinga, toxic to Aedes (Stegomyia) aegypti (Linnaeus) (Diptera, Culicidae)

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