Genetic variation among South Brazilian accessions of Lippia alba Mill. (Verbenaceae) detected by ISSR and RAPD markers

Manica-Cattani, M. F.; Zacaria, Jucimar; Pauletti, Gabriel Fernandes; Atti-Serafini, L.; Echeverrigaray, Sérgio

doi:10.1590/s1519-69842009000200020

Cited by 36 publications

(31 citation statements)

References 23 publications

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“…Observed high level of genetic variability in this species, which were also detected by Manica-Cattani et al (2009) that studied 27 genotypes of L. alba through molecular markers : Harvest × progeny Interaction; σ P 2 : progeny variance; σ E 2 : Environmental variance = error b of ANOVA; CV G% : Genetic coeffient variation among half-sib progenies; CV E% : Environmental variation coefficient; h r 2 (%): narrow-sense heritability; b: Relationship CV G% / CV E% ; G S : absolute gain under 30% of selection intensity; G S% : selection gain related to the mean; LDM: dry mass of leaves per plant; TDM: total dry mass per plant; LY: leaves yield; EOY: oil yield; OP: oil production per plant. To make correlation estimates more robust, all progenies for all three harvests were taken into account in the ANOVAs (Table 4).…”

Section: Resultssupporting

confidence: 62%

Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Bottignon

Rufino

Marques

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Lippia alba (Mill.) N.E.Br. is an aromatic and medicinal shrub native to the American continent. Despite its potential as a source of essential oil for the pharmaceutical and cosmetics industries, few selection and genetic improvement studies have been carried out. The aim of this study was to provide genetic information on this species for breeding programs, showing its selection potential, by investigating clonal half-sib progenies. The following characteristics were evaluated per plant: leaf dry mass (LDM), total dry mass (TDM), leaf yield (LY), essential oil yield (EOY) and oil production (OP). Estimates were made for the several genetic parameters including absolute genetic gain at 30% selection intensity, correlations and relative contribution of additive and environmental effects to phenotypic correlation. Two experimental trials on 30 progenies were conducted: one in Campinas, state of São Paulo (SP), Brazil, with two harvests of the aerial part, and one in Monte Alegre do Sul, SP, Brazil, with only one harvest. The trials were conducted in a randomized block design consisting of subplots with three replications, each plot (progeny) consisting of 8 to 15 clonally-replicated plants with subplot harvesting. Variations were detected between progenies and harvests, as well as progeny/harvest interactions in the split plot experiment. High heritability and genetic gains were obtained at both sites for LDM, TDM and OP. The lowest variations among progenies were obtained for LY and EOY, highlighting selection problems. Negative additive genetic correlations were obtained for EOY × LDM, EOY × TDM, LY × TDM and LY × LDM. Selection for LDM resulted in increased oil production per plant (OP), even where there was a negative correlation between LDM × EOY.

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Section: Resultssupporting

confidence: 62%

Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Bottignon

Rufino

Marques

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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“…Many species of Verbenacea are used as important folk remedies for the treatment of several human health disorders, like some kinds of cancer and hypertension (Ghisalberti, 2000;Manica-Cattani et al, 2009). Due to the medicinal importance or toxic effects of several species of Verbenaceae, efforts have been made to develop markers that allow distinguishing among species.…”

Section: Introductionmentioning

confidence: 99%

Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

et al. 2017

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The family Verbenaceae hosts important species used in traditional medicine of many countries. The taxonomic controversies concerning the specific delimitation of several of its species make it difficult to guarantee the botanical origin of herbal preparations based on species of this family. To contribute to the development of both specific chemomarkers and a quality control tool to authenticate the botanical origin of herbal preparations of Verbenacea species, we determined the foliar HPLC-DAD phenolic profiles and the antioxidant properties of 10 wild species of this family occurring in Mexico. The contents of phenols and flavonoids varied significantly among species. Priva mexicana showed the highest levels of total phenolics (53.4 mg g -1 dry tissue) and Verbena carolina had the highest levels of flavonoids (17.89 mg g -1 dry tissue). Relevant antioxidant properties revealed by antiradical and reducing power were found for the analyzed species. These properties varied significantly in a species-dependent manner. The phenolic compounds accumulated were flavones and phenolic acids. Flavones were the only type of flavonoids found. The results of a cluster analysis showed that the compounds were accumulated in species-specific profiles. The phenolic profiles are proposed as valuable chemomarkers that can become a useful tool for the quality control concerning the botanical origin of herbal medicinal preparations based on the species analyzed. In addition, phenolic profiles could contribute importantly to solve the taxonomic controversies concerning species delimitation in the family Verbenaceae.Keywords: Verbenacea, antioxidant activity, chemomarkers, flavones, phenolic profiles. Compostos fenólicos das folhas de dez especies selvagem de Verbenaceae como antioxidantes e quimiomarcadores específicos ResumoA família Verbenaceae compreende importantes espécies utilizadas na medicina popular de muitos países. As dificuldades taxonômicas relativas à delimitação específica de muitas das suas espécies face difícil a verificar a origem botânico das preparações herbales baseadas nas espécies desta família. Para fazer uma contribuição ao desenvolvimento de indicadores taxonômicos e dum método de controle de qualidade para verificar a origem botânico de preparações herbales das espécies de Verbenaceae, os perfis fenólicos, obtidos pares HPLC-DAD, e as atividades antioxidantes das folhas de 10 espécies selvagens Mexicanas desta família foram determinados. Os conteúdos dos compostos fenólicos totais e dos flavonoides foram significativamente diferentes entre as espécies. Priva mexicana apresentou a maior quantidade de compostos fenólicos totais (53.4 mg g -1 amostra seca) e Verbena carolina apresentou a maior quantidade de flavonoides (17.89 mg g -1 amostra seca). Verifica-se importantes propriedades antioxidantes, como os resultados dos ensaios da capacidade antiradical e do poder redutor indicaram. As propriedades antioxidantes foram significativamente diferentes entre as espécies. Verificou-se que os compostos fe...

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“…Our results indicated that the 2 molecular markers investigated in this study, namely ISSR and RAPD, efficiently identified and discriminated the Achillea species, allowing the characterization of all the accessions under study. The efficiency of these molecular markers for genotype identification in other aromatic and medicinal plants has been reported (Mattioni et al, 2002;Torres et al, 2003;Fracaro et al, 2005;Manica-Cattani et al, 2009).…”

Section: Inter-and Intra-specific Genetic Diversity Of Yarrow Speciesmentioning

confidence: 99%

“…It has been shown that different markers might reveal different kinds of variation (Powell et al, 1996). RAPD markers (Williams et al, 1990) and ISSR markers (Zietkiewicz et al, 1994) have been used with success to identify and determine relationships at the species, population, and cultivar levels in many plant species, including several aromatic and medicinal plants (Mattioni et al, 2002;Nan et al, 2003;Fracaro et al, 2005;Manica-Cattani et al, 2009;de Lima et al, 2011). However, there are limited reports on the genetic relationships of Achillea species.…”

Section: Introductionmentioning

confidence: 99%

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

Ebrahimi¹,

Farajpour²,

Rahimmalek³

2012

Genet. Mol. Res.

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ABSTRACT. Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, of which 159 were polymorphic. The similarity coefficient among Achillea tenuifolia accessions ranged from 61 to 86%, and from 40 to 84% among A. santolina accessions. A low similarity was observed between these two species (mean similarity = 0.36%). This low similarity is consistent with their geographical distribution. According to the results of cluster and PCA analyses, the two species completely separated from each other. These markers will aid in the identification of elite genotypes for domestication and breeding programs.

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Genetic variation among South Brazilian accessions of Lippia alba Mill. (Verbenaceae) detected by ISSR and RAPD markers

Cited by 36 publications

References 23 publications

Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

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