Effect of α-difluormethylornithine on Anastrepha fraterculus (Diptera, Tephritidae) ovary size

Abstract

Ovarian sizes (length and width) were measured in young females of Anastrepha fraterculus (Wiedemann) (Diptera, Tephritidae) subjected or not to the inhibitor α-difluormethylornithine (α-DFMO). The most effective concentration of α-DMFO used was 50 mM and the ovarian measurements (length and width) of the treated females were smaller than those of females not treated with α-DMFO. These data may suggest some relationship between ornithine decarboxylase (ODC) and sexual maturation in A. fraterculus.Keywords: Anastrepha fraterculus, α-difluormethylornithine, ovarian size . Efeito da α-difluorometilornitina nas dimensões dos ovários emAnastrepha fraterculus (Diptera, Tephritidae) ResumoAs dimensões dos ovários (comprimento e largura) foram mensuradas em fêmeas jovens da Anastrepha fraterculus (Wiedemann) (Diptera, Tephritidae) submetidas ou não ao inibidor α-difluormetilornitina (α-DFMO). A concentração mais efetiva de α-DMFO utilizada foi 50 mM e as medidas (comprimento e largura) das fêmeas tratadas com o inibidor foram menores que as fêmeas não tratadas com inibidor α-DMFO. Estes dados podem sugerir uma relação entre ornitina descarboxilase (ODC) e maturação sexual em A. fraterculus.Palavras-chave: Anastrepha fraterulus, α-difluoro metil ornitina; tamanho do ovário.

Ornithine decarboxylase deficiency critically impairs nitrogen metabolism and survival in Aedes aegypti mosquitoes

“…The RNAi‐driven depletion of ODC also strongly impaired nitrogen metabolism and reproduction in blood‐fed A. aegypti females. It was previously reported that the administration of DFMO negatively impacts insect reproduction 16,17,22,25 . Oocyte development was inhibited in A. aegypti females fed an artificial BM supplemented with 75 mM DFMO 25 .…”

“…It was previously reported that the administration of DFMO negatively impacts insect reproduction. 16,17,22,25 Oocyte development was inhibited in A. aegypti females fed an artificial BM supplemented with 75 mM DFMO. 25 A reduction in midgut trypsin activity and a decrease in DNA, RNA, polyamine, and Vg synthesis were also observed in blood-fed A. aegypti females treated 25 Digestive serine proteases such as LT and 5G1 trypsins play a major role during BM protein digestion in A. aegypti.…”

“…14 A family of polyamine-induced proteins called antizymes and their inhibitors are also important regulators of ODC activity. 14,15 ODC and polyamines have been implicated in different physiological processes including vitellogenesis, lipid metabolism, locomotor function, and aging in fruit flies [16][17][18][19][20][21] ; oviposition behavior in crickets 22 ; olfactory function in diamondback moths 23 ; and cell cycle progression in cultured silkworm cells. 24 It was also reported that chemical inhibition of ODC disrupted polyamine synthesis, as evidenced by the decreased conversion of radiolabeled ornithine into putrescine, spermidine, and spermine in Aedes aegypti mosquitoes following DL-αdifluoromethylornithine (DFMO) treatment.…”