Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Zara, Fernando José; Caetano, Flávio Henrique

doi:10.1590/s1519-69842004000400022

Cited by 16 publications

(18 citation statements)

References 11 publications

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“…Histological analyses show that urocytes of M. quadrifasciata pupae have a well-developed centralized and spherical nucleus with decondensed chromatin and cytoplasm lattice similar to urocytes described for the ant Pachycondyla villosa (Ponerinae) (Zara & Caetano, 2004) and for the cricket T. cavicola (Lipovsek et al, 2011). However, ultrastructural analyses showed that the nucleus has small lobes, as reported for the adult stingless bee Melipona bicolor (Paes-de-Oliveira & Cruz-Landim, 2003), Manduca sexta (Lepidoptera) pupae (Ehresmann et al, 1990), and Prorhinotermes simplex (Isoptera) (Sobotnik et al, 2006).…”

Section: Discussionsupporting

confidence: 60%

Histochemistry and Ultrastructure of Urocytes in the Pupae of the Stingless BeeMelipona quadrifasciata(Hymenoptera: Meliponini)

Furtado¹,

Azevedo²,

Martins³

et al. 2013

Microsc Microanal

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The main cell types of the adult bee fat body are trophocytes and oenocytes; however, in pupae of some newly emerged bees, trophocytes are modified into cells called urocytes, which possibly function as a substitute for Malpighian tubules during metamorphosis when larval tubules are not functional and/or storage of urate salts is required. This study evaluated the morphology of urocytes in the stingless bee Melipona quadrifasciata and the possibility of maintaining these cells in primary culture. The urocytes M. quadrifasciata are white spherical cells with an irregular surface as observed by stereomicroscopy. They may be found individually or in groups associated with tracheae. Urocytes have a single, small, and spherical nucleus and cytoplasm rich in neutral polysaccharides, lipid droplets, protein, and granules containing calcium and urate salts. Our findings suggest that urocytes play a role in storage of neutral polysaccharides and calcium in M. quadrifasciata pupae and that these cells can be cultured for 72 h.

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Section: Discussionsupporting

confidence: 60%

Histochemistry and Ultrastructure of Urocytes in the Pupae of the Stingless BeeMelipona quadrifasciata(Hymenoptera: Meliponini)

Furtado¹,

Azevedo²,

Martins³

et al. 2013

Microsc Microanal

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“…In the present work, the trophocytes displayed variable phenotypes, sharing histological features with the fat body cells reported in other insect species (Dean et al 1985, Zara and Caetano 2004, Roma et al 2010, Martins et al 2011). As expected, the bloodmeal lead to remarkable changes in the tissue which showed larger trophocytes with abundant lipid droplets, in agreement with the physiological role of the tissue and with its higher triacylglycerol content (Canavoso et al 2004, Pontes et al 2008).…”

Section: Discussionsupporting

confidence: 85%

The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

Fruttero

Leyria

Moyetta

et al. 2019

Journal of Insect Science

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In insects, lipid transfer to the tissues is mediated by lipophorin, the major circulating lipoprotein, mainly through a nonendocytic pathway involving docking receptors. Currently, the role of such receptors in lipid metabolism remains poorly understood. In this work, we performed a histological characterization of the fat body of the Chagas’ disease vector, Panstrongylus megistus (Burmeister), subjected to different nutritional conditions. In addition, we addressed the role of the β-chain of ATP synthase (β-ATPase) in the process of lipid transfer from lipophorin to the fat body. Fifth-instar nymphs in either fasting or fed condition were employed in the assays. Histological examination revealed that the fat body was composed by diverse trophocyte phenotypes. In the fasting condition, the cells were smaller and presented a homogeneous cytoplasmic content. The fat body of fed insects increased in size mainly due to the enlargement of lipid stores. In this condition, trophocytes contained abundant lipid droplets, and the rough endoplasmic reticulum was highly developed and mitochondria appeared elongated. Immunofluorescence assays showed that the β-ATPase, a putative lipophorin receptor, was located on the surface of fat body cells colocalizing partially with lipophorin, which suggests their interaction. No changes in β-ATPase expression were found in fasting and fed insects. Blocking the lipophorin–β-ATPase interaction impaired the lipophorin-mediated lipid transfer to the fat body. The results showed that the nutritional status of the insect influenced the morphohistological features of the tissue. Besides, these findings suggest that β-ATPase functions as a lipophorin docking receptor in the fat body.

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“…However, some also disperse their oenocytes amongst the tissue of the fat body, while others such as Coleoptera (beetles) organize them in a continuous streak running the length of the abdomen (Chapman, 1998; Wheeler, 1892). There are even descriptions of grasshoppers and ants with oenocytes spreading to thoracic segments late in their respective larval/nymph stages (Wheeler, 1892; Zara and Caetano, 2004). Thus, the variation in final location and number of larval oenocytes between insect species provides a potentially useful model for performing comparative evolutionary-developmental biology studies.…”

Section: Introductionmentioning

confidence: 99%

Oenocyte development in the red flour beetle Tribolium castaneum

et al. 2012

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Oenocytes are a specialized cell type required for lipid processing, pheromone secretion, and developmental signaling. Their development has been well characterized in Drosophila melanogaster, but it remains unknown whether the developmental program is conserved in other insect species. In this study, we compare and contrast the specification and development of larval oenocytes between Drosophila and the red flour beetle, Tribolium castaneum. First, we identify several useful reagents to label larval oenocytes, including both a Tribolium GFP enhancer trap line and a simple flurophore-conjugated streptavidin staining method that recognizes oenocytes across insect species. Second, we use these tools to describe oenocyte development in Tribolium embryos, and our findings provide evidence for conserved roles of MAP-kinase signaling as well as the Spalt, Engrailed, HNF4, and Ventral veins lacking factors in producing abdominal-specific oenocyte cells. However, Tribolium embryos produce four-times as many oenocytes per abdominal segment as Drosophila, and unlike in Drosophila, these cells rapidly down-regulate the expression of the Spalt transcription factor. Thus, these results provide new insight into the molecular pathways regulating oenocyte specification across insect species.

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Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Cited by 16 publications

References 11 publications

Histochemistry and Ultrastructure of Urocytes in the Pupae of the Stingless BeeMelipona quadrifasciata(Hymenoptera: Meliponini)

Histochemistry and Ultrastructure of Urocytes in the Pupae of the Stingless BeeMelipona quadrifasciata(Hymenoptera: Meliponini)

The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

Oenocyte development in the red flour beetle Tribolium castaneum

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