Morphology and anatomy of leaf mine in Richterago riparia Roque (Asteraceae) in the campos rupestres of Serra do Cipó, Brazil

Abstract

The leaf mine in Richterago riparia is caused by a lepidopteran larva (lepidopteronome). The leaves of R. riparia show campdodrome venation; the epidermis is unistratified, with stomata and glandular trichomes in adaxial and abaxial surfaces. The mesophyll is bilateral and the vascular system is collateral. During the formation of the mine, the larva consumes the chlorenchyma of the mesophyll and the smaller vascular bundles (veins of third and fourth orders). Structural alterations in the tissues of the host plant were not observed, except for the formation of a wound meristem and the presence of cells with phenolic substances next to the mine. Three cephalic exuviae of the miner were found in the mesophyll. This lepidopteronome is parenchymatic and the epidermis remains intact, but forms a protective layer for the mining insect.

Morphology and anatomy of a leaf mine in Vismia guianensis (Aubl.) Choisy (Clusiaceae) in a fragment of Brazilian Atlantic forest

“…some larvae are adept to avoiding latex and resinous cells which can have toxic chemicals that not only deter non-host insects but have little effect on adapted leaf miners. Melo-de-pinna et al (2002) discussed the absence of the new tissue formation in the mine of the Richterago riparia and they also pointed out that these facts corroborate with what was postulated by hering (1951), that mines are galls at an incipient stage of differentiation. according to Mani (1964) the plant tissue may be passive or they react with some cell proliferation.…”

“…the leaf mine of V. guianensis occur in both surface epidermis, likely to have been observed by Melo-de-pinna et al (2002) in Richterago riparia (asteracea). the authors observed that larvae consumed the parenchyma tissue and are characterized as a parenchymatic mine according to hering (1951).…”

“…the nature biotic is well illustrated by miners of Richterago riparia (Melo-de-pinna et al, 2002) and of Vismia guianensis.…”

“…the first paper on the morphology and anatomy of leaf mine in Brazilian plants native to serra do Cipó was Melo-de-pinna et al (2002) in Richterago riparia (asteraceae). in this paper, the v/v) (Johansen, 1940) for 48 h. after fixation they were dehydrated in 50% and 70% ethanol.…”

What is the influence of ordinary epidermal cells and stomata on the leaf plasticity of coffee plants grown under full-sun and shady conditions?

“…The leaves were fixed and stored as previously described in Melo de Pinna et al (2002). Five samples of each treatment at the mid-lamina region (areas in the vicinity of large veins were avoided) were treated with chloroform for 2 hours, by shaking, and then rinsed in ethanol 70% and tap water.…”