Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

Höfling, José Francisco; Rosa, Edvaldo Antônio Ribeiro; Pereira, Cassiano Costa Silva; Boriollo, Marcelo Fabiano Gomes

doi:10.1590/s1519-69842001000300021

Cited by 11 publications

(10 citation statements)

References 39 publications

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“…Although the colonisation by Candida albicans is common and causes severe injuries in immunocompromised patients, other Candida species have been isolated from such patients, healthy patients and children with Down's syndrome, such as C. glabrata, C. krusei, C. tropicalis, C. lusitaniae, C. parapsilosis, C. guilliermondii (Erköse and Erturan, 2007;Höfling et al, 2001;Ribeiro et al, 2006;Rodrigues et al, 2004) and C. dubliniensis. These yeasts were recognised as the source of mucosal infection in HIV-positive patients and regarded as a significant cause of infections in humans, such as abdominal infections and fungemia (Erköse and Erturan, 2007).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial potential of some plant extracts against Candida species

et al. 2010

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The increase in the resistance to antimicrobial drugs in use has attracted the attention of the scientific community, and medicinal plants have been extensively studied as alternative agents for the prevention of infections. The Candida genus yeast can become an opportunistic pathogen causing disease in immunosuppressive hosts. The purpose of this study was to evaluate dichloromethane and methanol extracts from Mentha piperita, Rosmarinus officinalis, Arrabidaea chica, Tabebuia avellanedae, Punica granatum and Syzygium cumini against Candida species through the analysis of Minimum Inhibitory Concentration (MIC). Results presented activity of these extracts against Candida species, especially the methanol extract.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial potential of some plant extracts against Candida species

et al. 2010

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“…Data obtained by Höfling et al (2001) suggest that different nutritional compositions in the media, expressed by the electrophoretic profiles determine the expression of different proteins derived from alternative metabolic mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

et al. 2014

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The cellulase proteins have a great importance in the enzymatic hydrolysis of woody biomass. Despite of costs being a major concern, it has been a stimulus to study basidiomycetes biochemical properties which degrade lignocellulosic material and have prompted the processes' study for obtaining cellulolytic enzymes in fungi. The objective of this research was to evaluate the effects of the initial nitrogen content on (ammonium sulfate) and on sugar cane bagasse, which hereby, acts as an inducer of hydrolytic enzymes to produce cellulases and xylanases, using three Lentinula edodes (Berk.) Pegler strains as a transformation agent. A factorial design with 2 2 replications in the central point was conducted, varying concentrations of ammonium sulfate and sugar cane bagasse. The submerged cultures carried out in synthetic culture medium and incubated at 25°C for 7 days on an orbital shaker at 150 rpm. The total protein and cellulase activity as endoglucanase, exoglucanase and β-glucosidase and the xylanase was also determined. The results showed that the production of hydrolytic enzymes was stimulated by the presence of high concentrations of sugar cane bagasse (30g/L), characterizing it as an inducer due to the demonstrated proportional relationship. Thus, ammonium sulfate acted as a reducing agent in the synthesis of enzymes, being the low concentrations (0.1g/L) indicated for the enzyme production system under study. Among the studied strains, the EF52 showed higher activity for xylanase, endoglucanases, β-glucosidase and also protein.Keywords: basidiomycetes, sugar cane bagasse, ammonium sulfate, hydrolytic enzymes, proteins. Avaliação da produção de enzimas holocelulolíticas por Lentinula edodes (Berk.) Pegler durante o crescimento em fermentação submersa usando MSR ResumoAs celulases são proteínas de grande importância na hidrólise enzimática de biomassa florestal. No entanto, seu custo elevado tem estimulado o estudo de processos de obtenção de enzimas celulolíticas por fungos filamentosos, tais como os basidiomicetos que apresentam propriedades bioquímicas para degradação de material lignocelulósico. O objetivo deste trabalho foi avaliar os efeitos do teor inicial de nitrogênio (sulfato de amônia) e de um indutor de enzimas hidrolíticas (bagaço de cana de açúcar) na produção de xilanases e celulases utilizando três isolados de Lentinula edodes (Berk.) Pegler como agente de transformação. Foi realizado um planejamento fatorial 2 2 com repetição no ponto central, variando as concentrações de sulfato de amônia e bagaço de cana de açúcar. O cultivo submerso realizado em meio de cultivo sintético e incubado a 25°C por 7 dias em agitador orbital a 150 rpm. Foram determinados o teor de proteínas totais e a atividade de celulase como: endoglucanase, exoglucanase e β-glucosidase e ainda xilanase. Os resultados demonstraram que a produção das enzimas hidrolíticas foi estimulada pela presença de alta concentração de bagaço de cana (30g/L), caracterizando-o como agente indutor devido à relação de proporcionalidad...

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“…In regard to whole-cell proteins, their separation has been employed satisfactorily in the characterization of bacteria and yeast (13,22,26,55,56,57,58). Many investigators employed electrophoretic analysis of whole-cell proteins in the fungi taxonomy (19,21,49,58). The polyacrylamide gel electrophoresis also has been used in the identification of oral yeasts.…”

Section: Introductionmentioning

confidence: 99%

SDS-Page and numerical analysis of Candida albicans from human oral cavity and other anatomical sites

Rodrigues

Höfling

Boriollo

et al. 2004

Braz. J. Microbiol.

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The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity coefficient of simple matching and UPGMA algorithm, respectively. The results obtained showed several C. albicans subtypes and their similarity degrees (80% to 100%). Such data showed that same patients may be infected by two or more C. albicans subtypes in certain anatomical sites (i.e. only in oral cavity of immunocompromised patients, blood, or tracheal secretion), or yet, two or more patients can be infected in identical anatomical sites (i.e. bronchial washing, urine, oral cavity, tracheal secretion, vaginal secretion, and healthy saliva) with a same C. albicans subtype. However, two or more patients also can show infections in corresponding sites (i.e. oral cavity of immunocompromised patients, blood, oropharyngeal secretion, oral cavity, tracheal secretion, vaginal secretion, and healthy saliva) by different C. albicans subtypes. Besides, two or more patients also can be infected with identical or different C. albicans subtypes in different anatomical sites (i.e.1. identical subtypes in vaginal secretion, tracheal secretion, and urine; abdominal secretion and spittle; drainage and oral cavity; catheter and healthy saliva -i.e.2. subtypes different in bronchial washing, oropharyngeal secretion, pulmonary secretion, oral cavity of immunocompromised patients, and blood). Complementary studies involving C. albicans sample isolated from several anatomical sites of immunocompetent or immunocompromised patients (before, during and after specifics therapies) and their families or hospital workers must be done in order to establish the sources of C. albicans colonization. The whole-cell proteins profile performed by SDS-PAGE associated with computerassisted numerical analysis may provide preliminary criteria for taxonomic and epidemiological studies of such microorganisms.

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Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

Cited by 11 publications

References 39 publications

Antimicrobial potential of some plant extracts against Candida species

Antimicrobial potential of some plant extracts against Candida species

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

SDS-Page and numerical analysis of Candida albicans from human oral cavity and other anatomical sites

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