Atividade tóxica de isolados de Bacillus Thuringiensis a larvas de Aedes Aegypti (l.) (diptera: culicidae)

Costa, Juliana Regina Vieira da; Rossi, Juliana Regina; Marucci, Suzana Cristina; Alves, Eliane Cristina da Cunha; Volpe, Haroldo Xavier Linhares; Ferraudo, Antônio Sérgio; Lemos, Manoel Victor Franco; Desidério, Janete Apparecida

doi:10.1590/s1519-566x2010000500015

Cited by 20 publications

(16 citation statements)

References 5 publications

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“…Each native Bt isolates and the reference Bti -H14 strains were PCR-screened using five universal primers designed from conservative regions of the cry2 , cry4 , cry11 , cyt1 and cyt2 genes as previously described [31–33] as well as 13 primers specific to highly variable regions of the cry4a , cry4ba , cry10 , cyt1Aa , cyt1Ab , cyt2Aa , cyt2Ba , cry17 + 27 , cry24 + 40 , cry25 , cry29 , cry30 (Additional file 2: Table S2). The chi gene, known for its chitinase activity that may increase insecticidal toxicity [7], was also examined using a set of specific primers (Additional file 2: Table S2) .…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

et al. 2016

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BackgroundWorldwide, mosquito vectors are transmitting several etiological agents of important human diseases, including malaria, causing millions of deaths every year. In Saudi Arabia, as elsewhere, vector-control is based mostly on chemical insecticides which may be toxic and cause environmental deprivation. Here, to support the development of bio-pesticide alternatives, a study was conducted to identify native Bacillus thuringiensis (Bt) isolates with improved toxicity against the malaria vector, Anopheles gambiae (s.l.).MethodsSixty-eight Bt isolates were obtained from 300 soil and other samples collected from 16 sites across Saudi Arabia. Bt identification was based on morphological characteristics of colonies, shape of parasporal crystals and biochemical profiles. After characterization of their mosquitocidal activity, larvicidal strains were described through 16S ribosomal DNA gene sequencing, cry, cyt and chi genes PCR-amplification profiles, and SDS-PAGE protein analyses.ResultsSpherical Bt crystals were predominant amongst the 68 isolates (34%), while irregular, bi-pyramidal and spore-attached crystals were found in 32, 13 and 21% of strains, respectively. LC50 and LC90 bioassays showed that 23/68 isolates were larvicidal, with distinct biochemical activity profiles compared to non-larvicidal Bt strains. Eight larvicidal strains showed larvicidal activity up to 3.4-fold higher (LC50 range: 3.90–7.40 μg/ml) than the reference Bti-H14 strain (LC50 = 13.33 μg/ml). Of these, 6 strains had cry and cyt gene profiles similar to Bti-H14 (cry4Aa, cry4Ba, cry10, cry11, cyt1Aa, cyt1Ab, cyt2Aa). The seventh strain (Bt63) displaying the highest larvicidal activity (LC50 = 3.90 μg/ml) missed the cry4Aa and cyt1Ab genes and had SDS-PAGE protein profiles and spore/crystal sizes distinct from Bti-H14. The eight strain (Bt55) with LC50 of 4.11μg/ml had cry and cyt gene profiles similar to Bti-H14 but gave a chi gene PCR product size of 2027bp. No strains harbouring cry2, cry17 + 27, cry24 + 40, cry25, cry29, cry30, or cyt2Ba were detected.ConclusionThis study represents the first report of several Saudi indigenous Bt strains with significantly higher larvicidal efficacy against An. gambiae than the reference Bti-H14 strain. The very high toxicity of the Bt63 strain, combined with distinct cry and cyt genes and SDS-PAGE-protein profiles makes it a promising candidate for future applications in mosquito bio-control.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1922-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

et al. 2016

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“…Comparing our results with these, with the exception of the values reported by Sattar & Maitti (2011), the LC50 values of the strains evaluated in this study were up to 50 times lower. Da Costa et al (2010) suggested that the best strains of B. thuringiensis to control Aedes aegypti L. (Diptera: Culicidae) were those with the lowest values of both LC50 and LC90. Accordingly, we suggest that the best strains to control M. persicae are those with the smallest values of both LC50 and LC90.…”

Section: Discussionmentioning

confidence: 99%

Characterization ofBacillus thuringiensis(Bacillaceae) Strains Pathogenic toMyzus persicae(Hemiptera: Aphididae)

Torres-Quintero

Arenas-Sosa

Hernández-Velázquez

et al. 2016

Florida Entomologist

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“…In this study, among the 500 Bt isolates, six (1.2%) harbored cyt genes. In a previous study performed by Costa et al (2010) 1073 isolates were evaluated as the presence of dipteran specific genes. The frequency of cyt1Aa, cyt1Ab an cyt2Aa genes was 1.58%, 0,65% and 1,11%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Bacillus thuringiensis cyt Genes and Their Effect Against Fall Armyworm, Spodoptera frugiperda

Costa¹,

Lana²,

Barros³

et al. 2014

JAS

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Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.

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Atividade tóxica de isolados de Bacillus Thuringiensis a larvas de Aedes Aegypti (l.) (diptera: culicidae)

Cited by 20 publications

References 5 publications

Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Characterization ofBacillus thuringiensis(Bacillaceae) Strains Pathogenic toMyzus persicae(Hemiptera: Aphididae)

Molecular Characterization of Bacillus thuringiensis cyt Genes and Their Effect Against Fall Armyworm, Spodoptera frugiperda

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