Podisus nigrispinus requer cópulas longas para o sucesso reprodutivo

Abstract

-The occurrence of multiple and long matings seem to play an important role in the reproduction of the predatory stinkbugs such as Podisus nigrispinus (Dallas). However, the mechanisms underlying these behaviors remain unclear. In this study, the reproduction of P. nigrispinus was investigated as function of females` choice for their mating pair based on male body size and previous mating status, risk of predation and duration of mating. The female mating behavior was studied with female having multiple, partial or no partner choice based on male body size. Time to initiate a mating and its duration was observed after pairing males and females under risk of predation imposed by the presence of the predatory wasp Polistes versicolor Oliver. In addition, aiming to determine the reasons for long lasting matings, female had their mating interrupted at different intervals. Our data indicated that P. nigrispinus females do not select male partners as function of their body size and mating status. Duration of mating, mating partners' choice, and female fecundity and fertility were not infl uenced by the male size and risk of predation imposed by the predatory wasps. Mating interrupted after 30, 60, 120 and 240 min resulted in only 0, 3.1, 7.7 and 34% of egg hatching compared to 74.2% under uninterrupted mating (338 to 671 min long). Therefore, the reproductive success of P. nigrispinus females does not depend on male size and male mating status, but requires long-lasting mating as a condition for adequate spermatozoa transference to females instead. KEY WORDS: Asopinae, multiple mating, male size, reproduction RESUMO -A realização de múltiplas e longas cópulas parece ser importante para a reprodução de percevejos predadores como Podisus nigrispinus (Dallas), mas o comportamento governando essas características permanece desconhecido. Neste estudo foram investigados o comportamento de cópula e a reprodução de P. nigrispinus em função do tamanho do macho, seu status prévio de acasalamento, risco de predação e interrupção de cópulas. A escolha da fêmea para o acasalamento foi estudada sob múltipla escolha, parcial ou sem chance de escolha por machos grandes ou pequenos. O comportamento para o início do acasalamento e tempo de cópula sob risco de predação quando na presença de Polistes versicolor Oliver foi comparado àquele de casais sem risco de predação. Machos e fêmeas de P. nigrispinus acasalaram independentemente do tamanho e status prévio de acasalamento dos machos, além de não apresentarem preferência por parceiros. A duração da cópula, escolha do parceiro e fertilidade das fêmeas não foram infl uenciadas pelo tamanho do macho, nem a presença do predador alterou o comportamento de acasalamento e duração de cópula. Fêmeas com cópulas interrompidas após 30, 60, 120 e 240 min apresentaram baixa viabilidade de ovos (0; 3,1; 7,7 e 34%, respectivamente), enquanto aquelas com tempo natural de cópula (338 a 671 min) obtiveram 74,2% de viabilidade. Portanto, o sucesso reprodutivo de P. nigrispinus não depende do tam...

Mating behavior of the predator Podisus nigrispinus (Heteroptera: Pentatomidae) under exposure to neem: Comportamiento de apareamiento del depredador Podisus nigrispinus (Heteroptera: Pentatomidae) expuesto al neem

“…In agreement with this work, which was observed that exposure of the predator P. nigrispinus azadirachtin interferes with the mating behavior. P. nigrispinus mating are multiple and long and the prolonged period of mating is associated with the appropriate transfer of seminal material to females (Rodrigues et al, 2009).…”

“…Treated male had a reduced time of mating when treated alone. Thus, they may suffer a reduced sperm production and short-term transfer (Rodrigues et al, 2009).…”

“…However, the exposure of natural enemies to insecticide may affect its reproductive behavior and, thereafter, the control of insect pests (Desneux et al, 2004). Parameters such as duration and frequency of mating behavior have direct influence on the reproductive success of insects (Rodrigues et al, 2009), and insecticides may cause deviation in the acts of sequential mating of natural enemies (Claver et al, 2003). The objective of this study was to investigate the impact and possible consequences of azadirachtin on the mating behavior of the predator P. nigrispinus.…”

Lightweight males of Podisus nigrispinus (Heteroptera: Pentatomidae) neglect lightweight females due low reproductive fitness

“…This is important for Podisus stink bugs, which can copulate for up to 11 hours (Rodrigues et al, 2009) and assume a mating position that might increase their chances of being predated.…”

Stink Bugs (Pentatomidae)

“…One of the most common species in the Neotropical Region, P. nigrispinus has been found attacking several pest insects (De Clercq 2000 ). Several studies has been carried out regarding the biology, predation potential in different agroecosystems, and rearing methods of this species, also known as the Brazilian soldier spined bug (De Clercq 2000 ;Vivan et al 2003 ;Lemos et al 2003, De Medeiros et al 2004Sousa-Souto et al 2006 ;Torres et al 2006 ;Neves et al 2009 ;Rodrigues et al 2009 ;De Bortoli et al 2011 ;Soares et al 2011 ;Laumann et al 2013 ;Vacari et al 2013 ). The genus Supputius include three species, and S. cincticeps can be recognized by rostral segment II as long as III and IV combined, apex of scutellum concolorous with the disk and abdominal tubercle strongly produced, reaching between metacoxae (Thomas 1992 ).…”