Filamentous fungi associated with mosquito larvae (Diptera: Culicidae) in municipalities of the Brazilian Amazon

Pereira, Eleny da Silva; Sarquis, Maria Inez de Moura; Ferreira-Keppler, Ruth Leila; Hamada, Neusa; Alencar, Yamile B

doi:10.1590/s1519-566x2009000300009

Cited by 33 publications

(30 citation statements)

References 25 publications

(18 reference statements)

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“…Many authors have isolated fungi from insects (11,12,22,(26)(27)(28)(29)(30)(31)(32)(33). Other authors have studied the association of fungi with mosquitoes (4,10,24,34,35). Other fungal strains were isolated from insects.…”

Section: Discussionmentioning

confidence: 99%

“…Fungal isolation from the insect environment A sample of 0.1 mL was collected from the breeding water (immature breeding environment) and sucrose water, and from air (mature breeding environment), and spread separately on Czapek-Dox's agar plates using a sterilized scalpel, inside the laminar air flow hood. For fungal isolation from the air, the plates were exposed to air inside the cage for 30 minutes, incubated at 28°C for 5-7 days, and then, investigated for fungal identification (24).…”

Section: Internal Fungal Isolationmentioning

confidence: 99%

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Culex (Culex) Pipiens Mosquitoes Carry and Harbor Pathogenic Fungi during Their Developmental Stages

Galal¹,

AbuElnasr²,

Abdallah³

et al. 2017

Erciyes Med J

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Objective: Fungi are the main source of aflatoxin contamination in nature. The present study aimed to assess the role of the cosmopolitan mosquito species, Culex pipiens, in the circulation and dissemination of pathogenic fungi in nature, and to evaluate its capability to harbor these fungi. Materials and Methods: Fungi were isolated and identified from both, the external surface and the internal organs of the developmental stages and from the breeding environment of Cx. pipiens. Results: A total of 35 fungal isolates were isolated from both, the internal organs and the external surface of the developmental stages and from the breeding environment of Cx. pipiens. These isolates were identified as eleven Penicillium notatum isolates, eleven Aspergillus flavus isolates, six Rhizopus stolonifer isolates, four Candida albicans isolates, two Fusarium solani isolates, and one Aspergillus niger isolate. Antagonistic activity showed that the P. notatum growth inhibited the growth of the bacteria, Staphylococcus aureus. Conclusions: This study revealed that the different developmental stages of Cx. pipiens mosquito were capable of harboring many fungal species. Subsequently, this mosquito will be incriminated in the mechanical dissemination, circulation, and transmission of these fungi in nature, during its life cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Internal Fungal Isolationmentioning

confidence: 99%

Culex (Culex) Pipiens Mosquitoes Carry and Harbor Pathogenic Fungi during Their Developmental Stages

Galal¹,

AbuElnasr²,

Abdallah³

et al. 2017

Erciyes Med J

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“…The immature stages of anopheles are non-selective filter-feeders of organic particles suspended in the water suspension and of microorganisms such as bacteria, yeasts, protozoans and fungi (Pereira et al, 2009). Hence, the bacteria identified from the larva natural habitat may be constituent of the larva potential diet.…”

Section: Discussionmentioning

confidence: 99%

Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia

Chukalo¹,

Abate²

2017

Afr. J. Microbiol. Res.

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Mosquito larvae feed on particulate organic matter including microorganisms. This study was conducted to investigate the diversity and abundant bacteria of Anopheles mosquito larva breeding habitats and to evaluate the contribution of maize pollen as source of nutrients for bacterial growth. The nutrient composition (COD, NH 3 -N and TP) of the larva breeding habitats water samples were measured by HACH 2010. Bacteria were isolated and enumerated from 18 water samples of larva habitats. The abundance of bacteria in the larva breeding habitats was signi ficantly different. Bacteria were found to be grown abundant in habitats close to maize pollen sources (tasseled zone). This implies that maize pollen contributes to bacterial abundance. The Pearson Correlation showed that there was positive relationship between bacterial abundance and physicochemical characteristics of the water samples. The bacterial population in the habitat was dominated by species of Bacillus, Pseudomonas, Micrococcus and Serratia. The dominant bacteria were tested for their capability to grow on maize pollen medium. The growth kinetics of bacteria on maize pollen broth was performed to 18 h culture using JENWAY spectrophotometer at 600 nm wave length. The bacteria could show optimum growth on Maize Pollen broth at 15 g/l as of nutrient broth. The release of maize pollen during anthesis in rainy season in habitats close to larva breeding pool and its nutriment quality support proliferation of large array of bacteria which results in increased larval nourishment. Increased malaria transm ission in Asendabo could thus be caused as the bacteria serve as source of nutrients for mosquito larva.

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“…Fungi are very host specific in their mode of action. [13] The larvicidal activity/bioassay of each of the test fungi was elucidated. All of them were identified to be entomopathogenic and proved to be a potent larvicidal agent as they were capable of incapacitating the Culex spp larvae efficiently at the dose of 10 8 spores/ml.…”

Section: Discussionmentioning

confidence: 99%

Eradication of Mosquito Borne Diseases by Entomopathogenic Fungi

Majumder¹

2017

WJPR

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Entomopathogenic fungi are those fungi that specifically target insects and act as a parasite thus exterminating or incapacitating them. The pathogenicity of the fungal isolates, lower fungi-Fusarium spp, Aspergillus spp, Penicillium spp &Trichoderma spp, higher fungi-Agaricus spp & Leucoagaricus spp were effectively tested against the 3 rd and 4 th instar larvae of mosquito Culex spp. The concentration of fungi used was 10 8 spores / ml. Larval mortalities were evaluated on daily basis to check the effect of the respective fungi, they showed mortality within 24 to 72 hours of spore inoculation. The cause of the larval mortality was then ascertained by histopathological evaluation of the digestive system of the dead larvae as well as the microscopic evaluation of their body. The histopathology results showed disintegration of the epithelial cells and gut lining and microscopic evaluation revealed blockage of the siphon tube with fungal spores. The results connote that the Fusarium spp, Aspergillus spp, Penicillium spp, Trichoderma spp, Agaricus spp & Leucoagaricus spp are potent Culex spp larvicide and can prove to be a robust biocontrol agent.

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Filamentous fungi associated with mosquito larvae (Diptera: Culicidae) in municipalities of the Brazilian Amazon

Cited by 33 publications

References 25 publications

Culex (Culex) Pipiens Mosquitoes Carry and Harbor Pathogenic Fungi during Their Developmental Stages

Culex (Culex) Pipiens Mosquitoes Carry and Harbor Pathogenic Fungi during Their Developmental Stages

Bacterial populations of mosquito breeding habitats in relation to maize pollen in Asendabo, south western Ethiopia

Eradication of Mosquito Borne Diseases by Entomopathogenic Fungi

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