Caracterização dos hemócitos de operários de Nasutitermes coxipoensis(Holmgren) (Isoptera: Termitidae) e avaliação hemocitária após parasitismo por Metarhizium anisopliae

Cunha, Franklin Magliano da; Wanderley‐Teixeira, Valéria; Teixeira, Álvaro Aguiar Coelho; Albuquerque, Auristela C.; Alves, Luíz Carlos; Lima, E.A. Luna Alves

doi:10.1590/s1519-566x2009000200021

Cited by 7 publications

(5 citation statements)

References 9 publications

(9 reference statements)

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“…On day 14 post-infection, similar changes were observed for these haemocyte types, except that the proportion of GRs was significantly greater than the control. Similar results were obtained with different insect species when infected with different microorganisms (Faraldo et al, 2008;Cunha et al, 2009). …”

Section: B Thuringiensissupporting

confidence: 82%

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

El-Aziz

Awad

2010

Micron

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Section: B Thuringiensissupporting

confidence: 82%

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

El-Aziz

Awad

2010

Micron

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“…Adipohemocytes are cells found in insects and some tick species with varying sized and shape lipid droplets, throughout the entire cytoplasm, some mitochondria, and eccentric nucleus (Cunha et al, 2009). In Aedes aegypti , this cell type is considered the second most abundant and may have some granules (Hillyer et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Non-visualization of other cell types such as prohemocytes, spherulocytes and oenocytoids in the tick hemolymph after fungal infection is suggested to reflect the reduction in the concentration of these cell types considering that these cells do not have specific functions in the cellular immune response, and, for that reason, they are supposed to be more vulnerable to the pathogenic infection (Lavine and Strand, 2002; Cunha et al, 2009). Prohemocytes may also be in a lower concentration because these cells may have differentiated into granulocytes and plasmatocytes (Feitosa et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Hemocytes are extremely relevant for studies about interactions between the host and its pathogens (Jones, 1962; Crossley, 1975; Lackie, 1980; Gupta, 1986; Paskewitz and Christensen, 1996; Chen and Lu, 2018; Iacovone et al, 2018). Arthropods’ hemocytes classification and terminology were framed mostly based on insect studies, while this literature is still scarce for ticks (Nevermann et al, 1991; Borovicková and Hypsa, 2005; Araújo et al, 2008; Cunha et al, 2009; Laughton et al, 2011). Additionally, compared to insects, literature about the cell response of ticks challenged with pathogens is poorly explored.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes

et al. 2019

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Metarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present in the hemolymph of invertebrates are the cells involved in the immune response of arthropods. Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well as their role in response to the fungal infection is scarce. The present study aimed to analyze the hemocytes of R. microplus females after Metarhizium robertsii infection, using light and electron microscopy approaches associated with the cytotoxicity evaluation. Five types of hemocytes (prohemocytes, spherulocytes, plasmatocytes, granulocytes, and oenocytoids) were described in the hemolymph of uninfected ticks, while only prohemocytes, granulocytes, and plasmatocytes were observed in fungus-infected tick females. Twenty-four hours after the fungal infection, only granulocytes and plasmatocytes were detected in the transmission electron microscopy analysis. Hemocytes from fungus-infected tick females showed several cytoplasmic vacuoles with different electron densities, and lipid droplets in close contact to low electron density vacuoles, as well as the formation of autophagosomes and subcellular material in different stages of degradation could also be observed. M. robertsii propagules were more toxic to tick hemocytes in the highest concentration tested (1.0 × 10 8 conidia mL −1 ). Interestingly, the lowest fungus concentration did not affect significantly the cell viability. Microanalysis showed that cells granules from fungus-infected and uninfected ticks had similar composition. This study addressed the first report of fungal cytotoxicity analyzing ultrastructural effects on hemocytes of R. microplus infected with entomopathogenic fungi. These results open new perspectives for the comprehension of ticks physiology and pathology, allowing the identification of new targets for the biological control.

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“…Farm. LTDA, Caiçaras, Brazil) after drying the slides at room temperature for 1 h. The percentage of the different types of hemocytes (plasmatocytes, granulocytes, oenocytoides, spherulocytes, adipohemocytes and prohemocytes) was obtained by counting 300 cells for each sample, following the methods of Falleiros et al (2003) and Cunha et al (2009). The data was analyzed with a i-test using the SAS software version 9.0 (SAS Institute, 2001).…”

Section: Cellular Immunitymentioning

confidence: 99%

Impact of Bt cotton on the immune system and histology of the midgut of the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

et al. 2013

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Despite the efficiency of transgenic plants expressing Bacillus thuringiensis (Bt) toxins as insecticides against several lepidopterans, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is one species that presents low susceptibility to most Bt crops. This study investigated the effects of the Cry 1 Ac toxin expressed by Bt cotton in the midgul of S. frugiperda and its effects on the humoral and cellular immune responses. Three hypotheses were proposed and tested with contributing factors for the natural tolerance oíS. frugiperda: (i) midgut regenerative cells are activated by the CrylAc toxin, and thus renew the epithehal cells damaged by the protein, (ii) CrylAc increased production of nitric oxide or phenoloxidase in the hemolymph, and (iii) there are qualitative and quantitative variations in the hemocyte levels of S. frugiperda. Caterpillars were reared using Bt cotton (Acala 90B) and non-Bt isolines (Acala 90), from the first to the fourth instar. The Bt cotton promoted elongation of the epithehal cells in the midgut of S. frugiperda caterpillars. Hence, evidence only supported the hypothesised increase of phenoloxidase (ii) and qualitative and quantitative differences in hemocyte levels (iii) in insects that were fed with Bt and non-Bt cotton. These parameters seem to explain the low susceptibihty of S. frugiperda to CrylAc toxin and they are a viable set of responses for the evaluation of other xenobiotic factors.

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Caracterização dos hemócitos de operários de Nasutitermes coxipoensis(Holmgren) (Isoptera: Termitidae) e avaliação hemocitária após parasitismo por Metarhizium anisopliae

Cited by 7 publications

References 9 publications

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

Ultrastructural and Cytotoxic Effects of Metarhizium robertsii Infection on Rhipicephalus microplus Hemocytes

Impact of Bt cotton on the immune system and histology of the midgut of the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

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