Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)

Moretto, Geraldo; Arias, Maria Cristina

doi:10.1590/s1519-566x2005000300004

Cited by 16 publications

(17 citation statements)

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“…The number of restriction sites detected and their relative position on a restriction map are used to determine the mitochondrial haplotype (Avise, 1994). Moretto and Arias (2005) characterized the mtDNA from the subspecies Melipona quadrifasciata quadrifasciata and M. q. anthidioides. In this study specific mtDNA regions were amplified through PCR and further digested with restriction enzymes.…”

Section: Genetic Variability At Intra and Interspecific Levelsmentioning

confidence: 99%

Molecular markers as a tool for population and evolutionary studies of stingless bees

et al. 2006

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-Molecular markers are widely used in biology to address questions related to ecology, genetics and evolution. In bees, molecular studies addressing those issues have focused on Apis and Apis mellifera. Here we describe examples where molecular markers from mtDNA and microsatellite analyses were applied to stingless bees species. The data obtained, although in some cases preliminary, have already proven useful to infer hypotheses about phylogeny, population dynamics, species validity and the evolution of this group of bees. stingless bees / molecular marker / mitochondrial DNA / microsatellite / evolution / Apidae / Meliponini

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Section: Genetic Variability At Intra and Interspecific Levelsmentioning

confidence: 99%

Molecular markers as a tool for population and evolutionary studies of stingless bees

et al. 2006

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“…Waldschmidt et al (2002), also using RAPD markers in populations of M. quadrifasciata, observed two genetically distinct groups, one being composed of M. q. quadrifasciata and other by M. q. anthidioides. Different mtDNA restriction patterns were detected between both subspecies by Moreto and Arias (2005). Souza et al (2008) analyzed 155 colonies of both subspecies of M. quadrifasciata using RFLP in the cytochrome b gene and found an association between the RFLP pattern and the difference in tergal stripes of each subspecies.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and historical demography of the neotropical stingless beeMelipona quadrifasciata(Hymenoptera, Apidae): incongruence between morphology and mitochondrial DNA

et al. 2010

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2010Phylogeography and historical demography of the neotropical stingless bee Melipona quadrifasciata (Hymenoptera, Apidae):incongruence between morphology and mitochondrial DNA APIDOLOGIE, v.41, n.5, p.534-547, 2010 http://producao.usp.br/handle/BDPI/15811 Abstract -The stingless bees are among the most abundant and ecologically important social invertebrates in tropical communities. The Neotropical stingless bee Melipona quadrifasciata has two subspecies: M. quadrifasciata quadrifasciata and M. quadrifasciata anthidioides. The main difference between subspecies are the yellow metassomal stripes, which are continuous in M. q. quadrifasciata and discontinuous in M. q. anthidioides. Recently, two populations were described with continuous stripes and inhabiting clearly disjunct areas in relation to M. q. quadrifasciata. We sequenced 852 bp of the mtDNA COI gene from 145 colonies from 56 localities, and for the first time performed a detailed phylogeographic study of a neotropical stingless bee. Phylogenetic analyses revealed the existence of two clades exhibiting a south to north distribution: southern populations comprise the subspecies M. q. quadrifasciata, and northern populations are composed of M. q. anthidioides and two disjunct populations with continuous stripes. The divergence time of these two phylogroups was estimated between 0.233 and 0.840 million years ago in the Pleistocene, a period of climatic changes and geomorphological alterations in the Neotropical region. No evidence of genetic structure in relation to the tergal stripes was found, indicating that the morphological trait regarding the pattern of stripes on tergites is not an accurate diagnostic for the subspecies of M. quadrifasciata. biogeography / coalescence / tergal stripes / Melipona quadrifasciata / subspecies

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“…The fragment size of the seven amplifi ed mtDNA regions ranged from 950 bp to 2200 bp (Table 1) according to the expected (Francisco et al 2001, Moretto & Arias 2005. The restriction enzymes Bgl II, Bam HI, Cfo I and Eco RV did not cleave any fragment.…”

Section: Resultsmentioning

confidence: 99%

“…These results were consistent among all samples, thus not representing inter population variation. Recently, Moretto & Arias (2005) published a comparative study between the two subspecies of M. quadrifasciata by analyzing mtDNA restriction patterns. Polymorphisms between M. quadrifasciata quadrifasciata and M. quadrifasciata anthidioides were detected for COI region digested with Hinf I and Nde I, and CytB region digested with Dra I.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA polymorphism among populations of Meliponaquadrifasciata quadrifasciata Lepeletier (Apidae: Meliponini) from southern Brazil

Torres¹,

Arias²,

Moretto³

2009

Neotrop. entomol.

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-The geographical distribution of the Brazilian endemic stingless bee Melipona quadrifasciata quadrifasciata Lepeletier ranges from Rio Grande do Sul to Minas Gerais states. The objective of the present study was to verify mtDNA polymorphisms among samples of M. q. quadrifasciata collected in southern Brazil. Twenty nine colonies from three localities (Blumenau and Mafra/SC and Prudentópolis/ PR) were sampled. Seven mtDNA regions were amplifi ed and further digested with 15 restriction enzymes (PCR-RFLP). Five composite haplotypes were identifi ed, with two unique to samples from Prudentópolis and the remaining three to samples from Mafra and/or Blumenau.

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Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)

Cited by 16 publications

References 10 publications

Molecular markers as a tool for population and evolutionary studies of stingless bees

Molecular markers as a tool for population and evolutionary studies of stingless bees

Phylogeography and historical demography of the neotropical stingless beeMelipona quadrifasciata(Hymenoptera, Apidae): incongruence between morphology and mitochondrial DNA

Mitochondrial DNA polymorphism among populations of Meliponaquadrifasciata quadrifasciata Lepeletier (Apidae: Meliponini) from southern Brazil

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