Técnica para criação e manutenção da broca-do-café, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae), em laboratório

Abstract

Technique for Rearing and Maintenance of the Coffee Berry Borer, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) ABSTRACT-A laboratory rearing technique of Hypothenemus hampei (Ferrari) on natural diet, with fast and easy maintenance and reduced mechanical damage to insects was developed. The technique is suitable for producing insects to be used on bioassays for entomopathogenic fungi selection, because no antibiotics are added to the diet. The rearing containers are made with PVC tubes (∅10 cm x 25 cm length) and PVC caps; a nylon mesh (3 mm) is adapted to the lower part of the container to facilitate the collection of insects. From 350 to 400 ripe and bored coffee fruits should be put in each container. Every 48h, 30-120 insects/container can be collected and used in bioassays or the rearing maintenance. This technique allows obtaining great number of borers in natural diet, with low manipulation and high productivity.

Economic Injury Level for the Coffee Berry Borer (Coleoptera: Curculionidae: Scolytinae) Using Attractive Traps in Brazilian Coffee Fields

“…The fruits were from the coffee variety Catuai Vermelho IAC-15 grown in Ponte Nova, MG, sampling 5,000 bored and 20,000 nonbored fruits at the berry stage. The bored fruits were taken to the laboratory where they were kept in mass rearing conditions following Hirose and Neves (2002). The nonbored fruits were sun-dried until the desired storage humidity (10 Ð11%).…”

Redução da sobrevivência da broca-do-café, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae), e do seu ataque aos frutos de café pela pulverização com nim em laboratório

“…Os frutos contendo as brocas foram mantidos em laboratório (Hirose & Neves 2002), em condições controladas (25 ± 3ºC, 65 ± 15% UR e 12h fotofase). Após três dias, os frutos foram peneirados em malha de 2 mm de diâmetro, sobre um tecido de poliéster branco e as fêmeas mais ativas de H. hampei foram coletadas com um aspirador bucal.…”