Frequência de Escherichia coli e sua sensibilidade aos antimicrobianos em menores de cinco anos hospitalizados por diarreia aguda

Moura, Maria de Almeida Lélis de do Rosário S.; Mello, Maria Júlia Gonçalves de; Calábria, Waldylene Barbosa; Germano, Eliane Mendes; Maggi, Ruben Schindler; Correia, Jailson de Barros

doi:10.1590/s1519-38292012000200008

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Although DEC infections are usually self-limiting, persistent diarrhea, due to EAEC and aEPEC isolates, could require nutritional therapy and treatment with antimicrobial drugs. Among the 11 antimicrobial drugs tested in our study, resistant DEC isolates were observed only for ampicillin (43.6%), cotrimoxazole (29.5%), and gentamicin (2.6%), with most of the resistant isolates pertaining to the EAEC and EPEC pathotypes ( Table 6); this is in accordance with previous studies carried out in Brazil (61)(62)(63). High levels of resistance to ampicillin and cotrimoxazole have also been found among DEC isolates from other countries of South America, such as Peru and Mexico (64,65).…”

Section: Resultssupporting

confidence: 92%

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Dias

Santos

et al. 2016

APMIS

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The aim of the present study was to investigate the prevalence of Diarrheagenic Escherichia coli (DEC) pathotypes, a leading cause of diarrhea worldwide, among diarrheal and healthy children, up to 5 years of age, living in the city of Botucatu, São Paulo, Brazil. DEC, investigated by PCR detection of virulence factor-encoding genes associated with the distinct pathotypes, was isolated from 18.0% of the patients, and 19.0% of the controls, with enteroaggregative E. coli (EAEC), the most frequent pathotype, being detected in equal proportion between patients and controls (10.0%). Among the enteropathogenic E. coli (EPEC) isolates, only one isolate was able to produce the localized adherence pattern to HeLa cells, being thus the only typical EPEC identified. All the remaining EPEC were classified as atypical (aEPEC), and detected in 8.0% and 8.5% of the patients and controls, respectively. Regarding the serotypes, 26.5% of the analyzed EPEC isolates belonged to classical EPEC-serogroups, and the only two STEC found were serotyped as O26:H11 (patient) and O119:H7 (control). Antimicrobial susceptibility tests revealed that 43.6%, 29.5% and 2.6% of the DEC isolates were resistant to ampicillin, cotrimoxazole and gentamicin, respectively. Our data indicate that EAEC remains prevalent among children living in Botucatu, and revealed atypical EPEC as emerging putative diarrheal agents in this geographical region.

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Section: Resultssupporting

confidence: 92%

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Dias

Santos

et al. 2016

APMIS

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“…27 The presence of E. coli in H 2 O or food is indicative of contamination with human or animal feces. Contamination is very common and easily spreads in H 2 O, which can contaminate food when it comes in contact, thereby promoting congenital transmission of E. coli bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…26 E. coli is one of the few live microorganisms on Earth that are capable of producing all components found in basic organic compounds. 27 The presence of E. coli in H 2 O or food is indicative of contamination with human or animal feces. Contamination is very common and easily spreads in H 2 O, which can contaminate food when it comes in contact, thereby promoting congenital transmission of E. coli bacteria.…”

Section: Introductionmentioning

confidence: 99%

Facet-dependent photocatalytic and antibacterial properties of α-Ag₂WO₄crystals: combining experimental data and theoretical insights

Roca

Sczancoski

Nogueira

et al. 2015

Catal. Sci. Technol.

123

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In this paper, we have combined the various experimental results and first-principles calculations with a new and interesting discussion to explain the photocatalytic and antibacterial activities of α-Ag 2 WO 4 crystals, which were obtained using the microwave-hydrothermal (MH) method with anionic surfactants. The advantages of the insights gained through the present work are two-fold. First, the mechanism and origin of the photocatalytic and antibacterial activities can be rationalized. Second, this facile and controllable synthetic method is expected to encourage the synthesis of complex metal oxides with specific active facets, and these insights can contribute to the rational design of new materials for multifunctional applications. X-ray diffraction and Rietveld refinement analysis confirmed that all the crystals have an orthorhombic structure without deleterious phases. Ultraviolet-visible diffuse reflectance spectroscopy indicated the presence of intermediary energy levels and a variation in the optical band gap values (3.09-3.14 eV) with the crystal growth process.The geometry, electronic properties of the bulk, and surface energies of these crystals were evaluated using first-principles quantum mechanical calculations based on the density functional theory. The crystal shapes was experimentally and theoretically modeled based on Rietveld refinement data, emission scanning electron microscopy images, and Wulff construction. To obtain a wide variety of crystal shapes, the morphologies were gradually varied by tuning the surface chemistry, i.e., the relative stability of the faceted crystals. The growth mechanisms of different α-Ag 2 WO 4 crystals and their facet-dependent photocatalytic and antibacterial performances were explored in details. The combination of experimental and theoretical data revealed the presence of (110) and (011) planes with high surface energies together with the disappearance of faces related to the Ĳ010)/Ĳ010) planes in α-Ag 2 WO 4 crystals are key factors that can rationalize both the photocatalytic and antibacterial activities. The different activities may be attributed to the different number of unsaturated superficial Ag and W atoms capable of forming the main active adsorption sites. Finally, we discuss how knowledge of surface-specific properties can be utilized to design a number of crystal morphologies that may offer improved performance in various applications.

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“…The EAEC category, identified in this study is considered an emerging pathogen and is associated with acute diarrhea in both developed and developing countries (Moura et al, 2012). They do not secrete toxins but cause chronic or persistent diarrhea.…”

Section: Discussionmentioning

confidence: 96%

Microbiological water quality monitoring of four ponds from Lagoa do Sino Farm located in São Paulo State, Brazil

Evangelista

Souza

Ferreira

et al. 2021

Acta Sci. Biol. Sci.

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This study aimed to evaluate the microbiological quality of the water of four ponds used for irrigation on the Lagoa do Sino Farm, as well as to perform the genotypic characterization of virulence factors in Escherichia coli isolates. Sampling was conducted for 11 months, between 2015 and 2016. Samples were analyzed for the presence of thermotolerant coliforms, E. coli and heterotrophs. DNA was extracted from E. coli isolates, followed by genotypic characterization by polymerase chain reaction. Agricultural activities and pesticides used in the sampling period were documented in order to assess possible relationships between agricultural activities and microbiological water quality. The absence of suitable riparian vegetation around all the ponds was observed, benefiting the entry of organic matter and contaminants in the water body. A high index of thermotolerant coliforms in some months indicated the possibility of the transmission of pathogenic microorganisms in these ponds. The values found in some months were above the regulatory limits for water potability and water intended for irrigation. The agrochemicals used in the period seem to influence the results obtained. All 17 E. coli isolates showed at least one of the virulence genes estA, stx1, stx2, and aatA, indicating enterotoxigenic, enterohaemorrhagic or enteroaggregative nature. The presence of E. coli in the waters may be associated with the presence of animals. The water samples analyzed are not suitable for irrigation of vegetables that are consumed raw and/or low lying fruits ingested without skin removal. It is essential to broaden the control of the use of chemicals, as well as the preservation of riparian vegetation to improve the quality of water used in the farm's agricultural activities.

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Frequência de Escherichia coli e sua sensibilidade aos antimicrobianos em menores de cinco anos hospitalizados por diarreia aguda

Cited by 7 publications

References 16 publications

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Facet-dependent photocatalytic and antibacterial properties of α-Ag₂WO₄crystals: combining experimental data and theoretical insights

Microbiological water quality monitoring of four ponds from Lagoa do Sino Farm located in São Paulo State, Brazil

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Frequência de Escherichia coli e sua sensibilidade aos antimicrobianos em menores de cinco anos hospitalizados por diarreia aguda

Cited by 7 publications

References 16 publications

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E. coli as putative emerging diarrheal agents in children living in Botucatu, São Paulo State, Brazil

Facet-dependent photocatalytic and antibacterial properties of α-Ag2WO4crystals: combining experimental data and theoretical insights

Microbiological water quality monitoring of four ponds from Lagoa do Sino Farm located in São Paulo State, Brazil

Contact Info

Product

Resources

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Facet-dependent photocatalytic and antibacterial properties of α-Ag₂WO₄crystals: combining experimental data and theoretical insights