Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189

Abstract

Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.

Characterization and safety evaluation of partially purified bacteriocin produced by Escherichia coli E isolated from fermented pineapple Ananas comosus (L.) Merr

“…The highest bacteriocin activity was observed to be produced at early stationary phase, which is similar to those in bacteriocin production studies of both Gram-positive and Gram-negative bacteria [28,43]. The maximum yield of pyocin SA 189 produced by P. aeruginosa SA189 was obtained at late log phase and remained constant until the late stationary phase [41]. The amount of bacteriocin released in the culture medium is correlated with the quantity of biomass produced.…”

“…Pyocin SA 189, which is a bacteriocin produced by Gram-negative bacteria P. aeruginosa SA 189, showed antibacterial activity against a number of sensitive Gram-positive bacteria including S. aureus, S. pyogenes, and Listeria monocytogenes. However, no inhibitory activity against some species was observed such as Shigella dysenteriae and Salmonella typhi [41]. Microcin 25, excreted by E. coli strain isolated from human feces, showed that the antibiotic was active against E. coli, Salmonella, and Shigella strains of clinical origin by cross-streaking assay [42].…”

Production of Purified MRSAcin from Local Clinical Strain of Methicillin-Resistant Staphylococcus aureus and its Applicability as Antimicrobial

“…The result showed by Naz S et al, 25 of pH on the biological activity, pyocin SA189 remained stable within the pH range (2 to 10).…”

“…It was assayed by treating (MRSAcin) solution at (20,25,30,37 and 40) °C and activity was assayed after (10, 30 and 60) min at each of these temperatures. Activity also assayed after 15minutes at 121°C.…”

Genome Sequence of Pseudomonas chlororaphis Strain 189

“…Like PA23, P . chlororaphis 189 contained an array of biosynthetic pathways capable of producing metabolites involved in biocontrol, including hydrogen cyanide (6), phenazine (7), pyocin (8), pyrroloquinoline quinone (9), and cell wall degradative enzymes. Genes conferring the ability to produce surfactants and form biofilms (10) were also found.…”