A comparative study of coastal and clinical isolates of <italic>Pseudomonas aeruginosa</italic>

Abstract

Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium having a versatile metabolic potential and great ecological and clinical significance. The geographical distribution of P. aeruginosahas revealed the existence of an unbiased genetic arrangement in terrestrial isolates. In contrast, there are very few reports about P. aeruginosa strains from marine environments. The present work was aimed at studying the distribution of P. aeruginosa in coastal waters along the Indian Peninsula and understanding the environmental influence on genotypic, metabolic and phenotypic characteristics by comparing marine and clinical isolates. Of the 785 marine isolates obtained on selective media, only 32 (~4.1%) were identified as P. aeruginosa, based on their fatty acid methyl ester profiles. A low Euclidian distance value (< 2.5) obtained from chemotaxonomic analysis suggested that all the environmental (coastal and marine) isolates originated from a single species. While UPGMA analyses of AP-PCR and phenotypic profiles separated the environmental and clinical isolates, fatty acid biotyping showed overlapping between most clinical and environmental isolates. Our study revealed the genetic diversity among different environmental isolates of P. aeruginosa. While biogeographical separation was not evident based solely on phenotypic and metabolic typing, genomic and metatranscriptomic studies are more likely to show differences between these isolates. Thus, newer and more insightful methods are required to understand the ecological distribution of this complex group of bacteria.

Microbial Diversity and Phage–Host Interactions in the Georgian Coastal Area of the Black Sea Revealed by Whole Genome Metagenomic Sequencing

“…These species thrive in the habitats with oil spills or in decaying phytoplankton blooms [ 29 ], often being observed in the Black Sea as well [ 30 ]. Acinetobacter and Pseudomonas are ubiquitous bacteria in the environment, including the marine ecosystems [ 31 ], and are also known to cause drug-resistant-related community and hospital-acquired infections [ 32 , 33 ]. We assume that the high abundance of Acinetobacter and Pseudomonas in the Black Sea coastal waters can be considered as a risk factor for increased colonization and incidence of Acinetobacter and Pseudomonas infections in the community.…”

Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

“…Antimicrobial metabolites derived from natural resources can be considered a green and safe alternative against synthetic compounds to suppress these bacterial outbreaks [2,3,15]. On this account, P. aeruginosa could be considered an important species with high metabolic diversity having ecological and clinical significance [17,18,29]. Their structurally diverse secondary metabolites are already known to have inhibitory potential against aquaculture pathogens especially belonging to Vibrio and Aeromonas [3,30,31].…”

“…These antimicrobial compounds efficiently act on pathogens causing cell lysis, nitrous oxide or acetyl-CoA carboxylase inhibition [14]. Among various Pseudomonas sp., P. aeruginosa is the most studied strain due to their high abundance and metabolic diversity [4,[16][17][18]. Studies showed that P. aeruginosa produced a variety of compounds such as phenazine-1carboxylic acid (PCA), phenazine-1-carboxamide, (2E, 5E)phenyltetradeca-2,5-dienoate and pyorubrin with biological potential [13,15,19,20].…”

“…Moreover the ecological diversity offered by the marine environment may enrich the Pseudomonas spp. with the untapped resource of bioactive metabolites which need to be explored further [14,18]. Antimicrobial activity of P. aeruginosa derived from the marine habitats was as effective as synthetic antibiotics like tetracycline and bacitracin [24].…”

Bacteriological Assessment of Hospital Air and Selected Surfaces in Three Referral Hospitals in Makurdi Middle Belt Nigeria

“…Coronavirus disease (COVID-19) pandemic has provided a lot of information about air borne infection, there is need to also shed light on bacteria that are capable of causing infection from hospital premises also known as nosocomial infection. These infections often manifested within forty-eight hours after a patient is being admitted or thirty days after being discharge from the hospital (Anusree et al, 2015;WHO, 2002). Therefore, bacteria from air can cause infection on patients with compromised immunity, especially when the patients come close a very close range (that is, neglecting physical distancing) to another individual who is a carrier of bacteria that are potential hospital acquired pathogens.…”