Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh)

Abstract

The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L−1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

Technologies for the Selection, Culture and Metabolic Profiling of Unique Rhizosphere Microorganisms for Natural Product Discovery

“…str. 5/14 (99.85% sequence similarity) and was selected based on the yellow-orange pigmentation of the colony and the known capacity of members of the genus to catabolize herbicides [50]. Despite a highly pigmented pellet being obtained after centrifugation of the culture broth, the ethanolic extract was only slightly coloured and upon subsequent analysis by UPLC-DAD-QToF-MS did not reveal any molecular features of interest and further analysis was abandoned.…”

The Genome Analysis of Methylobacterium populi YC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

“…For the other reported QPE hydrolase, the specific activities of FeH from Rhodococcus ruber JPL-2 were 1.16 (U mg −1 ) for QPE with k cat / K m value of 3.25 mM −1 ·s −1 [ 48 ]; 4.12 ± 0.25 (U mg −1 ) of ChbH from Pseudomonas azotoformans QDZ-1, with k cat / K m value of 6.57 µM −1 ·s −1 [ 49 ]; 7.95 ± 0.22 (U mg −1 ) of FpbH from Aquamicrobium sp. FPB-1, with k cat / K m value of 12.85 µM −1 ·s −1 [ 25 ].…”

Microbial degradation of pesticide: A review

“…Up to 700-fold improvement was obtained, and the best variant hydrolyzes chlorpyrifos at a rate similar to that of the hydrolysis of paraoxon by wild-type OPH (Cho (Horemans et al, 2016). Aryloxyphenoxy Propanoate (AOPP) degrading strain Rhodococcus ruber JPL-2 was isolated by Hongming et al (2015). A novel esterase gene feh, encoding phenoxaprop-p-ethyl hydrolyzing carboxyesterase (FeH) was cloned from R. ruber JPL2.…”