Molecular typing of Mycobacterium bovis isolates: a review

Tuberculosis remains as the world’s biggest threat. In 2014, human tuberculosis ranked as a major infectious disease by the first time, overcoming HIV death rates. Bovine tuberculosis is a chronic disease of global distribution that affects animals and can be transmitted to humans by the consumption of raw milk, representing a serious public health concern. Despite the efforts of different countries to control and eradicate bovine tuberculosis, the high negative economic impact on meat and milk production chains remains, given the decreased production efficiency (approximately 25%), the high number of condemned carcasses, and increased animal culling rates. This scenario has motivated the establishment of official programs based on regulations and diagnostic procedures. Although Mycobacterium tuberculosis and Mycobacterium bovis are the major pathogenic species to humans and bovines, respectively, nontuberculous mycobacteria within the Mycobacterium genus have become increasingly important in recent decades due to human infections, including the ones that occur in immunocompetent people. Diagnosis of mycobacteria can be performed by microbiological culture from tissue samples (lymph nodes, lungs) and secretions (sputum, milk). In general, these pathogens demand special nutrient requirements for isolation/growth, and the use of selective and rich culture media. Indeed, within these genera, mycobacteria are classified as either fast- or slow-growth microorganisms. Regarding the latter ones, incubation times can vary from 45 to 90 days. Although microbiological culture is still considered the gold standard method for diagnosis, molecular approaches have been increasingly used. We describe here an overview of the diagnosis of Mycobacterium species in bovine milk.

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“…This technique enables the extensive amplification of a small amount of the DNA molecule in an exponential manner 81 .…”

Section: Molecular Diagnosismentioning

confidence: 99%

“…It runs with two sets of primer pairs used in two continuous PCRs. The second run targets the final product 81 .…”

Section: Molecular Diagnosismentioning

confidence: 99%

Diagnosis of mycobacteria in bovine milk: an overview

Bolaños

Paula

Guerra

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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“…The in-vitro anti-M. tuberculosis properties of the synthesized hydrazones and the metal complexes (1)(2)(3)(4)(5) were evaluated against M. tuberculosis H37Rv (ATCC 35822) 34 and the results for the Minimum Inhibitory Concentrations (MICs) are reported in Table 4. The metal complexes tested displayed higher activity with MIC value of 0.74, 0.68 and 0.69 µg/mL for1, 2 and 3 respectively.…”

Section: Antimycobacterial Resultsmentioning

confidence: 99%

“…It is the leading cause of death due to a single infectious agent among human adults in the world 1 . Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), is one of the world's most devastating human pathogens, infecting one-third of the global population and claiming two million lives every year 2 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, Theoretical Treatment and Antitubercular activity Evaluation of (E)-N’- (2,5-dimethoxylbenzylidene)nicotinohydrazide and some of its Transition Metal Complexes against Mycobacterium tuberculosis, H37Rv

Kehinde¹,

Joseph²,

Tolutope³

et al. 2016

Orient. J. Chem

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( E)-N'-(2,5-dimethoxylbenzylidene)nicotinohydrazide) (HL) was synthesized by condensing nicotinic acid hydrazide and 2,5-dimethoxylbenzaldehyde with ONO coordination pattern. The structure of the hydrazone was elucidated by using CHN analyzer, ESI mass spectrometry, IR, 1 H NMR, 13 C NMR and 2D NMR (COSY and HSQC). The hydrazone was used to synthesized five metal complexes [Mo(V), VO(II), Mn(II) Ni(II) and Cu(II)] which were characterized by several physicochemical methods, namely elemental analysis, electronic spectra, infrared, EPR, molar conductivity and magnetic susceptibility measurements. An octahedral geometry was suggested for Mo(V), Ni(II) and Cu(II) complexes while tetrahedral was proposed for Mn(II) complex. VO(II) complex conformed with tetrahedral pyramidal. Structural geometries of these compounds were also suggested in gas phase by using Hyper Chem-8 program for the molecular mechanics and semiempirical calculations. The energy (E) and eV for homo and lumo state for the prepared compounds were calculated by using PM3 method. In vitro antimycobacterial activity study of the compounds was evaluated against Mycobacterium tuberculosis, H37Rv, by using micro-diluted method. Some of the metal complexes displayed higher activity than the ligand (HL) and isoniazid (INH).Also some of the complexes showed moderate activity when compared to isoniazid. Generally, the results obtained revealed that the compounds exhibited promising antitubercular activity. However, the metal complexes were found to be more toxic than isoniazid drug.

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“…Alguns autores defendem o desenvolvimento de vacinas para animais selvagens como uma medida de controle para esse grupo específico de animais(BUDDLE et al, 2013). Cabe salientar que as medidas de controle aplicadas à infecção por M. bovis em animais selvagens, deve-se também ao fato de que tais animais podem ser importantes reservatórios da doença reintroduzindo-a em rebanhos já controlados(MUSOKE et al, 2015) RAMOS et al, 2014)…”

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Investigação de possíveis grupos de risco para tuberculose humana por Mycobacterium bovis no estado de São Paulo

Couto¹

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Em primeiro lugar agradeço a Deus, que me iluminou e me proporcionou mais esta oportunidade. Agradeço em especial a meus pais, Maria Lúcia e Nilo Sérgio que contribuíram para a formação do meu caráter, pela amizade, apoio e amor acima de tudo. À minha irmã, Natália, pela amizade fraterna e eterna que me proporciona. À minha avó Maria Aparecida, à minha tia Alice e a minha Prima Cristina pela alegria que transmitem nos momentos em que estão presentes. Ao meu amigo Daniel Santos, por todos os momentos passados juntos. À minha amiga Luciana Casartelli que sempre esteve ao meu lado quando precisei e pela amizade sincera. A todos os meus amigos pelo apoio e por todos os momentos que estiveram presentes. Aos meus irmãos científicos e amigos, também alunos do prof. Eliseu, Márcia Tauil, Mônica Teixeira, Priscila Scraff e Kleydson Andrade e em especial à Jaqueline Lima, Eliana Masuda e Giulia Osório por todos os desabafos e momentos descontraídos. Aos profs. da Faculdade de Saúde Pública que muito contribuíram para meu aprendizado em saúde pública.

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Molecular typing of Mycobacterium bovis isolates: a review

Cited by 32 publications

References 53 publications

Diagnosis of mycobacteria in bovine milk: an overview

Diagnosis of mycobacteria in bovine milk: an overview

Synthesis, Characterization, Theoretical Treatment and Antitubercular activity Evaluation of (E)-N’- (2,5-dimethoxylbenzylidene)nicotinohydrazide and some of its Transition Metal Complexes against Mycobacterium tuberculosis, H37Rv

Investigação de possíveis grupos de risco para tuberculose humana por Mycobacterium bovis no estado de São Paulo

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