Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity

Abstract

A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis, Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.

Isolation of actinomycetes with antibacterial activity against multi-drug resistant bacteria

“…The most important biochemical effects of Dactinomycin are binding to single-and double-stranded DNA and subsequent inhibition of RNA and protein synthesis (Dobson et al, 2008). The antibacterial activity from previous studies (Shetty et al, 2014) supported our findings that Dactinomycin-like contained in the extract of OP2A-C is potent against most Gram-positive bacteria and MDR clinical isolates such as B. cereus ATCC9592.…”

Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa

“…To our knowledge, Streptomyces and Micromonospora species were reported capable of producing actinomycins (Kurosawa et al, 2006;Praveen, Tripathi, 2009). Antimycines were isolated for example from Streptomyces parvulus RSPSN2 (Keller et al, 2010), Streptomyces sindenensis (Praveen et al, 2008), Streptomyces avermitilis MS449 (Watkins et al, 1998), Streptomyces griseoruber (Shetty et al, 2014), Streptomyces nasri (Chen et al, 2012), Streptomyces chrysomallus (Sakiyama et al, 2014) and Streptomyces triostinicus (Praveen et al, 2009). There was a previous reports stating that also Streptomyces antibioticus produced actinomycin (El-Naggar, 1988; Singh et al, 2009) -however, not from South Africa and different species reported by different research groups may be the same species due to a lack of standard strain and not enough information regarding the characteristics of each strain (Shirling, Gottlieb, 1966).…”

A putative antimicrobial peptide from Hymenoptera in the megaplasmid pSCL4 of Streptomyces clavuligerus ATCC 27064 reveals a singular case of horizontal gene transfer with potential applications

“…Although some other peptides (especially with cyclic conformation) with antimicrobial and/or antitumoral effects have been reported in the Streptomyces genus (Shetty, Buddana, Tatipamula, Naga, & Ahmad, 2014;Um et al, 2013;Zhou et al, 2014), this is the first study to detect an AMP that is laterally transferred in this group of organisms. More than 2,500 natural or encrypted AMPs have been described previously (Zhang & Gallo, 2016).…”